Abstract:
:We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined with the function of such cells, this suggests that PA receptors interact with the extracellular matrix and that differentiation increases the number of PA-specific receptors, which supports previously observed differentiation-induced LT susceptibility. Our results quantifiably confirm that the generality of PA binding will complicate its use as a tumor targeting agent.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Deshpande A,Hammon RJ,Sanders CK,Graves SWdoi
10.1016/j.febslet.2006.06.070subject
Has Abstractpub_date
2006-07-24 00:00:00pages
4172-5issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00787-3journal_volume
580pub_type
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