Abstract:
:We studied the effect of acrolein, an alpha,beta-unsaturated aldehyde that causes adduct-modification of lysine, cysteine, and histidine residues, on antithrombin, a key anticoagulant serpin. Intrinsic fluorescence, functionality (anti-FXa and anti-IIa activity), heparin affinity and conformational features of plasma and purified antithrombin were evaluated. In vivo experiments were carried out in mice. Intrinsic fluorescence showed a two-step conformational change. Acrolein, even at low dose, impaired the anticoagulant function of purified antithrombin by affecting its heparin affinity. However, higher concentrations of acrolein and long incubations are required to cause mild functional effects on plasma antithrombin and mice.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Martínez-Martínez I,Ordóñez A,Guerrero JA,Pedersen S,Miñano A,Teruel R,Velázquez L,Kristensen SR,Vicente V,Corral Jdoi
10.1016/j.febslet.2009.07.062subject
Has Abstractpub_date
2009-10-06 00:00:00pages
3165-70issue
19eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00654-1journal_volume
583pub_type
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