Abstract:
:The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence of the apparent kinetic constant for the pre-steady state process was analyzed and an existence of multi-intermediates was proposed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kunugi S,Yokoyama M,Sakamoto Adoi
10.1016/0014-5793(95)00242-2subject
Has Abstractpub_date
1995-04-03 00:00:00pages
189-91issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(95)00242-2journal_volume
362pub_type
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