Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and codes for a sperm motility inhibitor protein.

abstract：:Muscarinic acetylcholine receptors purified from porcine atrium were phosphorylated, depending on the presence of agonists, by a protein kinase partially purified from porcine brain, which had similar properties to the beta-adrenergic receptor kinase. GTP-binding regulatory proteins (Go) had dual effects on the phosph...

journal_title：FEBS letters

authors： Haga K,Haga T

更新日期：1990-07-30 00:00:00

abstract：:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement...

journal_title：FEBS letters

authors： Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Y

更新日期：1996-04-22 00:00:00

abstract：:The BChla-containing B880-complex (core-complex) of Rhodopseudomonas marina (Rhodospirillaceae) was isolated with a new purification method. The isolation of the B880-complex was performed by solubilisation of the photosynthetic membranes with the detergent LDAO and subsequent fractionated ammonium-sulfate precipitati...

journal_title：FEBS letters

authors： Meckenstock RU,Brunisholz RA,Zuber H

更新日期：1992-10-19 00:00:00

abstract：:Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but ...

journal_title：FEBS letters

authors： Bermano G,Arthur JR,Hesketh JE

更新日期：1996-06-03 00:00:00

abstract：:Complementary (c) DNAs encoding a glycine receptor (GlyR) isomer were cloned from libraries constructed in lambda ZAPII with poly (A)+ RNA of neonatal rat spinal cord. Northern blot analysis revealed that RNA hybridized to the cloned cDNA is detectable only for a period of late embryonic/early postnatal stage of the s...

journal_title：FEBS letters

authors： Akagi H,Hirai K,Hishinuma F

更新日期：1991-04-09 00:00:00

abstract：:Avian red blood cells contain a millimolar amount of inositol polyphosphate which plays a role as an allosteric effector of hemoglobin. We confirmed the structure of this substance by NMR techniques as purely myo-inositol 1,3,4,5,6-pentakisphosphate. Based on present knowledge this effector is synthesized from inosito...

journal_title：FEBS letters

authors： Mayr GW,Dietrich W

更新日期：1987-03-23 00:00:00

abstract：:Using top-of-the-line fold recognition methods, we assigned an oligonucleotide/oligosaccharide-binding (OB)-fold structure to a family of previously uncharacterized hypothetical proteins from several bacterial genomes. This novel family of bacterial OB-fold (BOF) proteins present in a number of pathogenic strains enco...

journal_title：FEBS letters

authors： Ginalski K,Kinch L,Rychlewski L,Grishin NV

更新日期：2004-06-04 00:00:00

abstract：:When isolated reaction centres of photosystem II are subjected to photoinhibitory illumination, a 41 kDa SDS-PAGE band is observed under all experimental conditions. The same band is also found, together with lower molecular weight fragments of the D1 protein, in whole thylakoids and in all PSII sub-particles investig...

journal_title：FEBS letters

authors： Barbato R,Friso G,Rigoni F,Frizzo A,Giacometti GM

更新日期：1992-09-07 00:00:00

abstract：:Friedreich's ataxia is caused by a deficit in the mitochondrial protein frataxin. The present work demonstrates that in vivo yeast frataxin Yfh1p and Isu1p, the mitochondrial scaffold protein for the Fe-S cluster assembly, have tightly linked biological functions, acting in concert to promote the Fe-S cluster assembly...

journal_title：FEBS letters

authors： Ramazzotti A,Vanmansart V,Foury F

更新日期：2004-01-16 00:00:00

abstract：:Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pl...

journal_title：FEBS letters

authors： Inagaki NF,Inagaki FF,Kokudo N,Miyajima A

更新日期：2019-02-01 00:00:00

abstract：:A metallothionein cDNA was generated from pea (Pisum sativum L.) roots, amplified by PCR and inserted into a plasmid for expression in E. coli. Purification of the resultant product generated 3 pools of cadmium-containing material after DEAE-cellulose chromatography. The amino acid composition of each was in excellent...

journal_title：FEBS letters

authors： Kille P,Winge DR,Harwood JL,Kay J

更新日期：1991-12-16 00:00:00

abstract：:Extended X-ray absorption fine structure (EXAFS) spectra at the Cu K-edge have been recorded of the oxidized and reduced form at pH 3.5 of rusticyanin, the type-1 or 'blue'-copper protein from Thiobacillus ferrooxidans. The EXAFS of oxidized rusticyanin is well simulated with models assuming a ligand set of 2 N(His) a...

journal_title：FEBS letters

authors： Holt SD,Piggott B,Ingledew WJ,Feiters MC,Diakun GP

更新日期：1990-08-20 00:00:00

abstract：:The cAMP-dependent induction of 2,5-oligoadenylate (2-5A) synthetase and cAMP-dependent inhibition of 2-5A phosphodiesterase are shown. Variations in activities of cAMP-dependent protein kinase and the enzymes of 2-5A metabolism in the cells deepening into the resting state were found to be compatible with the above f...

journal_title：FEBS letters

authors： Itkes AV,Turpaev KT,Kartasheva ON,Tunitskaya VL,Kafiani CA

更新日期：1984-06-04 00:00:00

abstract：:In order to get a better insight into the function of amino acid residues located in the second transmembrane domain of the cystic fibrosis transmembrane conductance regulator (CFTR) protein, all exon 18 mutations found in cystic fibrosis (CF) patients were characterized at the protein and at the electrophysiological ...

journal_title：FEBS letters

authors： Vankeerberghen A,Wei L,Teng H,Jaspers M,Cassiman JJ,Nilius B,Cuppens H

更新日期：1998-10-16 00:00:00

abstract：:Cells of Saccharomyces cerevisiae were loaded with indo-1, by incubation in a medium of pH 4.5, which contained penta-potassium indo-1. Cells were then washed and resuspended in a buffer of pH 4.0. The emission fluorescence spectra were recorded between 390 and 500 nm (excitation at 355 nm) and the autofluorescent spe...

journal_title：FEBS letters

authors： Halachmi D,Eilam Y

更新日期：1989-10-09 00:00:00

abstract：:A complete cDNA clone encoding human cholesterol 7 alpha-hydroxylase has been isolated using a rat P-450ch7 alpha cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr 57,630), 39-base pair 5'-untranslated...

journal_title：FEBS letters

authors： Noshiro M,Okuda K

更新日期：1990-07-30 00:00:00

abstract：:The O2-dependent formation of side products during the oxidation of veratryl alcohol (VA) by lignin peroxidase has previously been proposed to start with the attack of H2O on the VA radical cation (VA*+). This initial reaction is unlikely since it would also lead to side product formation in the absence of O2, which i...

journal_title：FEBS letters

authors： ten Have R,Franssen MC

更新日期：2001-01-05 00:00:00

abstract：:In the new view, hydrophobic free energy is measured by the work of solute transfer of hydrocarbon gases from vapor to aqueous solution. Reasons are given for believing that older values, measured by solute transfer from a reference solvent to water, are not quantitatively correct. The hydrophobic free energy from gas...

journal_title：FEBS letters

authors： Baldwin RL

更新日期：2013-04-17 00:00:00

abstract：:The yeast Saccharomyces cerevisiae contains two acetyl-CoA synthetase genes, ACS1 and ACS2. While ACS1 transcription is glucose repressible, ACS2 shows coregulation with structural genes of fatty acid biosynthesis. The ACS2 upstream region contains an ICRE (inositol/choline-responsive element) as an activating sequenc...

journal_title：FEBS letters

authors： Hiesinger M,Wagner C,Schüller HJ

更新日期：1997-09-22 00:00:00

abstract：:Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...

journal_title：FEBS letters

authors： Rathmann C,Schlösser AS,Schiller J,Bogdanov M,Brüser T

更新日期：2017-09-01 00:00:00

abstract：:The hepatocyte growth factor/scatter factor (HGF/SF) receptor consists of an alpha- and a beta-subunit, which are derived from a single-chain precursor by endoproteolytic processing. The precursor is not proteolytically processed in LoVo colon carcinoma cells. The uncleaved receptor immunopurified from the cells was c...

journal_title：FEBS letters

authors： Komada M,Hatsuzawa K,Shibamoto S,Ito F,Nakayama K,Kitamura N

更新日期：1993-08-09 00:00:00

abstract：:Extracellular regulated kinases (ERKs) mediate the inhibitory effect of tumor necrosis factor alpha (TNF-alpha) on uncoupling protein-1 (UCP-1), but not on lipid accumulation. TNF-alpha-induced ERK-dependent peroxisome proliferator activator receptor gamma (PPAR gamma) phosphorylation could be responsible for UCP-1 do...

journal_title：FEBS letters

authors： Porras A,Valladares A,Alvarez AM,Roncero C,Benito M

更新日期：2002-06-05 00:00:00

abstract：:cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phospho...

journal_title：FEBS letters

authors： Temmei Y,Uchida S,Hoshino D,Kanzawa N,Kuwahara M,Sasaki S,Tsuchiya T

更新日期：2005-08-15 00:00:00

abstract：:Glycinebetaine (betaine), an osmoregulant in halophilic plants, stabilized the evolution of oxygen and the synthesis of ATP by thylakoid membranes from the cyanobacterium Synechocystis PCC6803 when it was present during the preparation and incubation of the thylakoid membranes. Moreover, betaine enhanced the evolution...

journal_title：FEBS letters

authors： Mamedov MD,Hayashi H,Wada H,Mohanty PS,Papageorgiou GC,Murata N

更新日期：1991-12-09 00:00:00

abstract：:The photoreduction of the quinone (Q) pool in the photosynthetic membrane of the purple bacterium Rhodobacter sphaeroides was investigated by steady-state and time-resolved Fourier transform infrared difference spectroscopy. The results are consistent with the existence of a homogeneous Q pool inside the chromatophore...

journal_title：FEBS letters

authors： Mezzetti A,Leibl W,Breton J,Nabedryk E

更新日期：2003-02-27 00:00:00

abstract：:RB, p53 and p21(Sdi1/WAF1/Cip1) interact in the induction of G1 arrest. We established osteosarcoma cell lines in which a tetracycline-regulatable promoter controls the induction of RB, p53 and p21. By using these cell lines, we investigated whether RB, p53 or p21 regulates, in the same manner or differently, expressi...

journal_title：FEBS letters

authors： Ookawa K,Tsuchida S,Kohno T,Yokota J

更新日期：2001-06-29 00:00:00

abstract：:The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with poly...

journal_title：FEBS letters

authors： Creazzo TL,Wrenn RW

更新日期：1988-12-19 00:00:00

abstract：:alpha-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (Mr 49 000), extracellularly as a complex-type glycoprotein (Mr 54 000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. Mr (41 000). The sequence of 26 N-terminal amino acids of rat alpha 1-antitryps...

journal_title：FEBS letters

authors： Gross V,Kaiser C,Tran-Thi TA,Schmelzer E,Witt I,Plummer TH Jr,Heinrich PC

更新日期：1983-01-24 00:00:00

abstract：:Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...

journal_title：FEBS letters

authors： Houston P,Goodman J,Lewis A,Campbell CJ,Braddock M

更新日期：2001-03-09 00:00:00

abstract：:We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length p...

journal_title：FEBS letters

authors： Agrotis A,Condron M,Bobik A

更新日期：2000-02-04 00:00:00