The effect of the natural protein inhibitor on H+-ATPase hepatoma 22a mitochondria.

abstract：:The cytochrome bc(1) complex catalyzes electron transfer from ubiquinol to cytochrome c by a protonmotive Q cycle mechanism in which electron transfer is linked to proton translocation across the inner mitochondrial membrane. In the Q cycle mechanism proton translocation is the net result of topographically segregated...

journal_title：FEBS letters

authors： Hunte C,Palsdottir H,Trumpower BL

更新日期：2003-06-12 00:00:00

abstract：:A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...

journal_title：FEBS letters

authors： Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJ

更新日期：1994-08-08 00:00:00

abstract：:DNA-dependent RNA polymerase from blood forms and culture forms of the parasitic protozoan Trypanosoma brucei was resolved into multiple peaks of activity by DEAE-Sephadex chromatography. The enzyme from the two forms was found to be different by several criteria, suggesting that it is subject to developmental control...

journal_title：FEBS letters

authors： Tittawella I

更新日期：1988-01-25 00:00:00

abstract：:Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene cod...

journal_title：FEBS letters

authors： Bittel C,Tabares LC,Armesto M,Carrillo N,Cortez N

更新日期：2003-10-23 00:00:00

abstract：:PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII d...

journal_title：FEBS letters

authors： Kawakami K,Iwai M,Ikeuchi M,Kamiya N,Shen JR

更新日期：2007-10-16 00:00:00

abstract：:A novel calcium-dependent serine proteinase (CASP) secreted from malignant hamster embryo fibroblast Ni 12C2 degrades extracellular matrix proteins. A complementary DNA encoding CASP has been isolated with the use of oligonucleotide probes synthesized based on partial amino acid sequences of CASP. The complete amino a...

journal_title：FEBS letters

authors： Kinoshita H,Sakiyama H,Tokunaga K,Imajoh-Ohmi S,Hamada Y,Isono K,Sakiyama S

更新日期：1989-07-03 00:00:00

abstract：:More than 40 years have passed since the original publication by Hayflick and Moorhead led to the concept of the 'Hayflick limit' of the maximum number of divisions which somatic cells undergo in vitro. This concept is still regarded as a fundamental characteristic of species longevity. Here we want to emphasize anoth...

journal_title：FEBS letters

authors： Yegorov YE,Zelenin AV

更新日期：2003-04-24 00:00:00

abstract：:The function of the domains of Trichoderma reesei endoglucanase I (EGI) has been studied. Truncated EGI proteins were expressed from the 3'-end deleted cDNAs in the yeast Saccharomyces cerevisiae under the control of the ADC1 expression cassette. EGI protein was detected by monoclonal antibody EI-2 and EGI activity as...

journal_title：FEBS letters

authors： Aho S

更新日期：1991-10-07 00:00:00

abstract：:The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using c...

journal_title：FEBS letters

authors： Ninomiya M,Suganuma M,Paik NS,Muto Y,Fujiki H

更新日期：1988-06-20 00:00:00

abstract：:In brain tissue a spectrin-like calmodulin-binding protein calspectin, or fodrin, is concentrated in a synaptosome fraction, where most of the calspectin is associated with the synaptic membranes. This endogenous calspectin was phosphorylated by protein kinase system(s) associated with the membranes. Here, we report t...

journal_title：FEBS letters

authors： Sobue K,Kanda K,Kakiuchi S

更新日期：1982-12-13 00:00:00

abstract：:In response to Ca(2+) signaling, cytosolic phospholipase A(2)alpha (cPLA(2)alpha) translocates from the cytosol to the perinuclear membrane, where downstream eicosanoid-synthetic enzymes, such as cyclooxygenase (COX), are localized. Although the spatiotemporal perinuclear colocalization of cPLA(2)alpha and COXs has be...

journal_title：FEBS letters

authors： Murakami M,Das S,Kim YJ,Cho W,Kudo I

更新日期：2003-07-10 00:00:00

abstract：:Chronic pain and epilepsy together affect hundreds of millions of people worldwide. While traditional pharmacotherapy provides essential relief to the majority of patients, a large proportion remains resistant, and surgical intervention is only possible for a select few. As both disorders are characterised by neuronal...

journal_title：FEBS letters

authors： Snowball A,Schorge S

更新日期：2015-06-22 00:00:00

abstract：:A mutant of Escherichia coli has been described that produces an altered form of penicillin-binding protein 5 which still binds penicillin but is unable to catalyse the release of the bound penicilloyl moiety. We show that the mutation is caused by a single nucleotide transition that results in a change from glycine a...

journal_title：FEBS letters

authors： Broome-Smith J,Spratt BG

更新日期：1984-01-09 00:00:00

abstract：:4-Picoloine-2,2':6',2"-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 x 10(7) M(-1) for the most stable binding mode of 4-picoline-2,2':6',2"-terpyridine-platinum(II) to poly[d(A-T)2...

journal_title：FEBS letters

authors： McCoubrey A,Latham HC,Cook PR,Rodger A,Lowe G

更新日期：1996-02-12 00:00:00

abstract：:Despite their differential cell tropisms, HIV-1 and HCV dramatically influence disease progression in coinfected patients. Macrophages are important target cells of HIV-1. We hypothesized that secreted HCV core protein might modulate HIV-1 replication. We demonstrate that HCV core significantly enhances HIV-1 replicat...

journal_title：FEBS letters

authors： Swaminathan G,Pascual D,Rival G,Perales-Linares R,Martin-Garcia J,Navas-Martin S

更新日期：2014-09-17 00:00:00

abstract：:We investigated transgenic tobacco lines which express different amounts of the barley JIP 23. In these plants the amount of several proteins decreased proportionally to increasing amounts of JIP 23 whereas the transcript levels were constant as determined for the small and the large subunit of RuBPCase. However, the ...

journal_title：FEBS letters

authors： Görschen E,Dunaeva M,Reeh I,Wasternack C

更新日期：1997-12-08 00:00:00

abstract：:RKIP was first identified as an inhibitor of the Raf-MEK-ERK signaling pathway. RKIP was also found to play an important role in the NF-kappaB pathway. Genetic and biochemical studies demonstrated that RKIP functioned as a scaffold protein facilitating the phosphorylation of IkappaB by upstream kinases. However, contr...

journal_title：FEBS letters

authors： Tang H,Park S,Sun SC,Trumbly R,Ren G,Tsung E,Yeung KC

更新日期：2010-02-19 00:00:00

abstract：:Avian red blood cells contain a millimolar amount of inositol polyphosphate which plays a role as an allosteric effector of hemoglobin. We confirmed the structure of this substance by NMR techniques as purely myo-inositol 1,3,4,5,6-pentakisphosphate. Based on present knowledge this effector is synthesized from inosito...

journal_title：FEBS letters

authors： Mayr GW,Dietrich W

更新日期：1987-03-23 00:00:00

abstract：:In this work, we have spectroscopically characterised CYP157C1 from Streptomyces coelicolor A3(2) which has the motif E(297)QSLW(301) rather than the invariant EXXR motif in the P450 K-helix. Site-directed mutagenesis of native E(297)QSLW(301) in CYP157C1 to E(297)ESLR(301) or E(297)QSRW(301) both containing standard ...

journal_title：FEBS letters

authors： Rupasinghe S,Schuler MA,Kagawa N,Yuan H,Lei L,Zhao B,Kelly SL,Waterman MR,Lamb DC

更新日期：2006-11-27 00:00:00

abstract：:Using a cationic lipid bilayer, we show that DNA can be reliably adsorbed to the bilayer surface for atomic force microscopy (AFM) in aqueous buffers at high resolution. The measured width of the dsDNA is close to 2 nm, and a periodic modulation on dsDNA is reproducibly detected by the AFM. The measured period is 3.4 ...

journal_title：FEBS letters

authors： Mou J,Czajkowsky DM,Zhang Y,Shao Z

更新日期：1995-09-11 00:00:00

abstract：:A collagenase cleaving native type I [14C]collagen but inactive against the synthetic substrate Pz-Pro-Leu-Gly-Pro-D-Arg was extracted from mineralized human dental tissue. The enzyme specifically degrades native collagen into characteristic products (3/4) and (1/4). Its apparent molecular mass of 68 kDa is relatively...

journal_title：FEBS letters

authors： Dumas J,Hurion N,Weill R,Keil B

更新日期：1985-07-22 00:00:00

abstract：:A distinctive tract of all the forms of Alzheimer's disease is the extracellular deposition of a 40-42/43 amino acid-long peptide derived from the so-called beta-amyloid precursor protein (APP). This is a membrane protein of unknown function, whose short cytosolic domain has been recently demonstrated to interact with...

journal_title：FEBS letters

authors： Russo T,Faraonio R,Minopoli G,De Candia P,De Renzis S,Zambrano N

更新日期：1998-08-28 00:00:00

abstract：:The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Stre...

journal_title：FEBS letters

authors： Wang YY,Ran XX,Chen WB,Liu SP,Zhang XS,Guo YY,Jiang XH,Jiang H,Li YQ

更新日期：2014-08-25 00:00:00

abstract：:The possibility that the phospholipid-N-methyltransferases from yeast are capable of acting upon a phospholipid substrate, localized in a different membrane than in which the enzymes reside ('trans-catalysis' hypothesis), was investigated using cho2 and opi3 gene disruptant strains, which are defective in phosphatidyl...

journal_title：FEBS letters

authors： Janssen MJ,de Jong HM,de Kruijff B,de Kroon AI

更新日期：2002-02-27 00:00:00

abstract：:The effects of metal cations on the activation of recombinant human SK4 (also known as hIK1 or hKCa4) channels, expressed in HEK 293 cells, were tested using patch clamp recording. Of the nine metals tested, cobalt, iron, magnesium, and zinc did not activate the SK4 channels when applied, at concentrations up to 100 m...

journal_title：FEBS letters

authors： Cao YJ,Houamed KM

更新日期：1999-03-05 00:00:00

abstract：:Under conditions of molar excess of enzyme, isolated F1-ATPase from beef heart mitochondria catalyses ATP hydrolysis biphasically. The rate constants for product release are approximately 10(-1) and 10(-4)-10(-3) s-1, respectively. The slow phase of ATP hydrolysis is insensitive to EDTA. [gamma-32P]ATP splitting in th...

journal_title：FEBS letters

authors： Milgrom YaM,Murataliev MB

更新日期：1987-09-28 00:00:00

abstract：:Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...

journal_title：FEBS letters

authors： Suzuki T,Kovacs MS,Board PG

更新日期：1990-11-26 00:00:00

abstract：:The products of the isiAB operon are a chlorophyll antenna protein (IsiA) and flavodoxin (IsiB), which accumulate in cyanobacteria grown under iron starvation conditions. Here we show that strong light triggers de-repression of isiAB transcription and leads to IsiA and flavodoxin accumulation under iron replete condit...

journal_title：FEBS letters

authors： Havaux M,Guedeney G,Hagemann M,Yeremenko N,Matthijs HC,Jeanjean R

更新日期：2005-04-25 00:00:00

abstract：:Ferric reductase B (FerB) is a flavin mononucleotide (FMN)-containing NAD(P)H:acceptor oxidoreductase structurally close to the Gluconacetobacter hansenii chromate reductase (ChrR). The crystal structure of ChrR was previously determined with a chloride bound proximal to FMN in the vicinity of Arg101, and the authors ...

journal_title：FEBS letters

authors： Sedláček V,Kučera I

更新日期：2019-04-01 00:00:00

abstract：:We have previously shown that Rap1 as well as its guanine nucleotide exchange factor Epac1 increases cell-cell junction formation. Here, we show that activation of Epac1 with the exchange protein directly activated by cAMP (Epac)-specific cAMP analog 8CPT-2'O-Me-cAMP (007) resulted in a tightening of the junctions and...

journal_title：FEBS letters

authors： Kooistra MR,Corada M,Dejana E,Bos JL

更新日期：2005-09-12 00:00:00