Abstract:
:The long form of human uncoupling protein-3 (hUCP3L) is highly homologous to thermogenin (UCPI), the uncoupling protein of brown fat mitochondria, but is expressed predominantly in skeletal muscle. Its putative role is to regulate the coupling efficiency of oxidative phosphorylation and thus thermogenesis in skeletal muscle, a major thermogenic tissue in higher mammals. To study the functional relevance of hUCP3L, the protein was expressed in yeast cells under the control of the galactose promoter. Expression of hUCP3L induced a series of phenotype changes in the yeast cells. The cellular growth and the mitochondrial membrane potential were both diminished. The portion of cellular respiration coupled to oxidative phosphorylation decreased from 57% to 11% (P<0.001) and the cellular heat production, as measured by direct microcalorimetry, was increased by 33.3 +/- 3.2% (P<0.001) after induction of UCP3L. These observations demonstrate for the first time the intrinsic thermogenic properties of hUCP3L in intact cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hinz W,Faller B,Grüninger S,Gazzotti P,Chiesi Mdoi
10.1016/s0014-5793(99)00331-2keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
57-61issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00331-2journal_volume
448pub_type
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