The first plant type III polyketide synthase that catalyzes formation of aromatic heptaketide.

Abstract:

:A cDNA encoding a novel plant type III polyketide synthase (PKS) was cloned from rhubarb (Rheum palmatum). A recombinant enzyme expressed in Escherichia coli accepted acetyl-CoA as a starter, carried out six successive condensations with malonyl-CoA and subsequent cyclization to yield an aromatic heptaketide, aloesone. The enzyme shares 60% amino acid sequence identity with chalcone synthases (CHSs), and maintains almost identical CoA binding site and catalytic residues conserved in the CHS superfamily enzymes. Further, homology modeling predicted that the 43-kDa protein has the same overall fold as CHS. This provides new insights into the catalytic functions of type III PKSs, and suggests further involvement in the biosynthesis of plant polyketides.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Abe I,Utsumi Y,Oguro S,Noguchi H

doi

10.1016/S0014-5793(04)00230-3

keywords:

subject

Has Abstract

pub_date

2004-03-26 00:00:00

pages

171-6

issue

1-3

eissn

0014-5793

issn

1873-3468

pii

S0014579304002303

journal_volume

562

pub_type

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