The first plant type III polyketide synthase that catalyzes formation of aromatic heptaketide.


:A cDNA encoding a novel plant type III polyketide synthase (PKS) was cloned from rhubarb (Rheum palmatum). A recombinant enzyme expressed in Escherichia coli accepted acetyl-CoA as a starter, carried out six successive condensations with malonyl-CoA and subsequent cyclization to yield an aromatic heptaketide, aloesone. The enzyme shares 60% amino acid sequence identity with chalcone synthases (CHSs), and maintains almost identical CoA binding site and catalytic residues conserved in the CHS superfamily enzymes. Further, homology modeling predicted that the 43-kDa protein has the same overall fold as CHS. This provides new insights into the catalytic functions of type III PKSs, and suggests further involvement in the biosynthesis of plant polyketides.




FEBS letters


Abe I,Utsumi Y,Oguro S,Noguchi H





Has Abstract


2004-03-26 00:00:00














  • Congenital jaundice in rats due to the absence of hepatic bilirubin UDP-glucuronyltransferase enzyme protein.

    abstract::Three major UDP-glucuronyltransferase isoenzymes (50-54 kDa) have been identified by immunoblot analysis. Bilirubin UDP-glucuronyltransferase (54 kDa) was specially induced by treatment of the rats with clofibrate. This isoenzyme was not detectable in liver microsomal extracts from congenitally jaundiced Gunn rats and...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Scragg I,Celier C,Burchell B

    更新日期:1985-04-08 00:00:00

  • Heme incorporation into the cytochrome bo3 occurs at a late stage of assembly.

    abstract::Respiratory complexes in both prokaryotes and eukaryotes contain multiple co-factors, which are coordinated in defined positions so that they can function as electron wires. Intriguingly, co-factors are usually buried deep within hetero-oligomeric protein complexes and it is not clear when or how they are incorporated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Palombo I,Daley DO

    更新日期:2012-11-30 00:00:00

  • CMP-N-acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron-sulphur protein to be described in Eukarya.

    abstract::Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schlenzka W,Shaw L,Kelm S,Schmidt CL,Bill E,Trautwein AX,Lottspeich F,Schauer R

    更新日期:1996-05-06 00:00:00

  • 8-Chloro-dGTP, a hypochlorous acid-modified nucleotide, is hydrolyzed by hMTH1, the human MutT homolog.

    abstract::The human mutT homolog, hMTH1, suppresses spontaneous mutations by degrading the endogeneous mutagen, 8-hydroxy-dGTP. We previously reported the broad substrate specificity of hMTH1, which also degrades the oxidatively damaged purine nucleotides, 2-hydroxy-dATP, 8-hydroxy-dATP, 2-hydroxy-ATP, and 8-hydroxy-GTP, in add...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fujikawa K,Yakushiji H,Nakabeppu Y,Suzuki T,Masuda M,Ohshima H,Kasai H

    更新日期:2002-02-13 00:00:00

  • Molecular cloning and expression of subunit 9 of the 26S proteasome.

    abstract::Seven peptides from subunit 9 (S9) of the human 26S proteasome were sequenced and this information was used to clone a HeLa cDNA that encodes the 46 kDa subunit. Rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from S9 and against a full-length S9 expressed in E. coli. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hoffman L,Rechsteiner M

    更新日期:1997-03-10 00:00:00

  • Uber die quantitative Bestimmung von Sterinderivaten in pflanzen und die intrazelluläre Verteilung der Steringlycoside in Blättern.

    abstract::The contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (Daucus carota L.) potato (Solanum tuberosum L.), onion (Allium cepa L.) two varieties of radish (Raphanus sativus L.) and Gentiana ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eichenberger W,Grob EC

    更新日期:1970-12-01 00:00:00

  • Limited proteolysis of cytochrome c in trifluoroethanol.

    abstract::Horse heart cytochrome c is cleaved by thermolysin in 50% aqueous TFE (v/v) at neutral pH (25 degrees C, 24 h) at the Gly56-Ile57 peptide bond of the 104-residue chain of the protein. Additional, but anyway minor, fragmentation at the Gly45-Phe46 and Met80-Ile81 peptide bonds is also observed. On the other hand, in bu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fontana A,Zambonin M,De Filippis V,Bosco M,Polverino de Laureto P

    更新日期:1995-04-10 00:00:00

  • N-linked glycosylation of Drosophila rhodopsin occurs exclusively in the amino-terminal domain and functions in rhodopsin maturation.

    abstract::Immature Drosophila rhodopsin is N-glycosylated, but undergoes complete deglycosylation during the process of protein maturation. In order to elucidate the site of glycosylation and its role in rhodopsin synthesis, we investigated the in vitro and in vivo synthesis of rhodopsin whose putative N-glycosylation sites (As...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Katanosaka K,Tokunaga F,Kawamura S,Ozaki K

    更新日期:1998-03-13 00:00:00

  • IL-1 beta, a strong mediator for glucose uptake by rheumatoid and non-rheumatoid cultured human synoviocytes.

    abstract::Higher basal 2-deoxy-D-glucose uptake in rheumatoid synovial cells than in non-rheumatoid synovial cells, was found to be associated with an increased interleukin-1 beta (IL-1 beta) secretion (respectively 850 +/- 238 vs. 8.3 +/- 2.4 pg/24 h/10(5) cells, mean +/- S.E.M.). When exogenous human recombinant IL-1 beta was...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hernvann A,Aussel C,Cynober L,Moatti N,Ekindjian OG

    更新日期:1992-05-25 00:00:00

  • Suppression of a phosphatidylinositol 3-kinase signal by a specific spliced variant of Drosophila PTEN.

    abstract::Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Maehama T,Kosaka N,Okahara F,Takeuchi K,Umeda M,Dixon JE,Kanaho Y

    更新日期:2004-05-07 00:00:00

  • Characterization of a 41 kDa photoinhibition adduct in isolated photosystem II reaction centres.

    abstract::When isolated reaction centres of photosystem II are subjected to photoinhibitory illumination, a 41 kDa SDS-PAGE band is observed under all experimental conditions. The same band is also found, together with lower molecular weight fragments of the D1 protein, in whole thylakoids and in all PSII sub-particles investig...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Barbato R,Friso G,Rigoni F,Frizzo A,Giacometti GM

    更新日期:1992-09-07 00:00:00

  • N-terminal amino acid sequences of precursor and mature forms of alpha-1-antitrypsin.

    abstract::alpha-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (Mr 49 000), extracellularly as a complex-type glycoprotein (Mr 54 000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. Mr (41 000). The sequence of 26 N-terminal amino acids of rat alpha 1-antitryps...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gross V,Kaiser C,Tran-Thi TA,Schmelzer E,Witt I,Plummer TH Jr,Heinrich PC

    更新日期:1983-01-24 00:00:00

  • Comparative EPR studies on the nitrite reductases from Escherichia coli and Wolinella succinogenes.

    abstract::Hexaheme nitrite reductases purified to homogeneity from Escherichia coli K-12 and Wolinella succinogenes were studied by low-temperature EPR spectroscopy. In their isolated states, the two enzymes revealed nearly identical EPR spectra when measured at 12 K. Both high-spin and low-spin ferric heme EPR resonances with ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Liu MC,Liu MY,Payne WJ,Peck HD Jr,Le Gall J,DerVartanian DV

    更新日期:1987-06-29 00:00:00

  • Histone H5 and H1 cross-reacting material is restricted to erythroid cells in chicken.

    abstract::Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shannon MF,Wigley PL,Wells JR

    更新日期:1985-07-08 00:00:00

  • Copolymers of glutamic acid and tyrosine are potent inhibitors of oocyte casein kinase II.

    abstract::Polypeptides rich in glutamic acid are strong inhibitors purified from isolated nuclei of Xenopus laevis oocytes of casein kinase II. The presence of tyrosine in these peptides greatly enhances their inhibitory capacity. Using casein as a substrate, copolyglu:tyr (4:1) has an I50 value of 20 nM, 250 fold lower than th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tellez R,Gatica M,Allende CC,Allende JE

    更新日期:1990-06-04 00:00:00

  • Monoclonal antibody evidence for structural similarities between the central rod regions of actinin and dystrophin.

    abstract::A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) fro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nguyen TM,Ellis JM,Ginjaar IB,van Paassen MM,van Ommen GJ,Moorman AF,Cartwright AJ,Morris GE

    更新日期:1990-10-15 00:00:00

  • Systematic screening of LNA/2'-O-methyl chimeric derivatives of a TAR RNA aptamer.

    abstract::We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Di Primo C,Rudloff I,Reigadas S,Arzumanov AA,Gait MJ,Toulmé JJ

    更新日期:2007-02-20 00:00:00

  • Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases.

    abstract::Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1+ and hxk2+ encoding these enzymes were cloned and sequenced. Disruption of hxk1+ had no effect on growth but d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Petit T,Blázquez MA,Gancedo C

    更新日期:1996-01-08 00:00:00

  • Regulation of ubiquitous 6-phosphofructo-2-kinase by the ubiquitin-proteasome proteolytic pathway during myogenic C2C12 cell differentiation.

    abstract::6-Phosphofructo-2-kinase catalyzes the synthesis and degradation of fructose 2,6-bisphosphate, activator of phosphofructokinase-1 and inhibitor of fructose 1,6-bisphosphatase. These properties confer to this bifunctional enzyme a key role in the control of glycolysis and gluconeogenesis. Several mammalian isozymes gen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Riera L,Obach M,Navarro-Sabaté A,Duran J,Perales JC,Viñals F,Rosa JL,Ventura F,Bartrons R

    更新日期:2003-08-28 00:00:00

  • In vitro PKA phosphorylation-mediated human PDE4A4 activation.

    abstract::The PDE4 catalytic machinery comprises, in part, two divalent cations in a binuclear motif. Here we report that PDE4A4 expressed in Sf9 cells exhibits a biphasic Mg(2+) dose-response (EC(50) of 0.15 and >10 mM) in catalyzing cAMP hydrolysis. In vitro phosphorylation of PDE4A4 by the PKA-catalytic subunit increases the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Laliberté F,Liu S,Gorseth E,Bobechko B,Bartlett A,Lario P,Gresser MJ,Huang Z

    更新日期:2002-02-13 00:00:00

  • DHS-21, a dicarbonyl/L-xylulose reductase (DCXR) ortholog, regulates longevity and reproduction in Caenorhabditis elegans.

    abstract::Dicarbonyl/L-xylulose reductase (DCXR) converts l-xylulose into xylitol, and reduces various α-dicarbonyl compounds, thus performing a dual role in carbohydrate metabolism and detoxification. In this study, we identified DHS-21 as the only DCXR ortholog in Caenorhabditis elegans. The dhs-21 gene is expressed in variou...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Son le T,Ko KM,Cho JH,Singaravelu G,Chatterjee I,Choi TW,Song HO,Yu JR,Park BJ,Lee SK,Ahnn J

    更新日期:2011-05-06 00:00:00

  • Both bulk and cancer stem cell subpopulations in triple-negative breast cancer are susceptible to Wnt, HDAC, and ERα coinhibition.

    abstract::Development of targeted therapies for triple-negative breast cancer (TNBC, a more aggressive subtype) is an unmet medical need. We analyzed data from 887 patients with invasive breast cancer and observed that increased Wnt and histone deacetylase (HDAC) activities are associated with estrogen receptor 1 (ESR1) and pro...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Sulaiman A,Sulaiman B,Khouri L,McGarry S,Nessim C,Arnaout A,Li X,Addison C,Dimitroulakos J,Wang L

    更新日期:2016-12-01 00:00:00

  • Thimet oligopeptidase: site-directed mutagenesis disproves previous assumptions about the nature of the catalytic site.

    abstract::Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chen JM,Stevens RA,Wray PW,Rawlings ND,Barrett AJ

    更新日期:1998-09-11 00:00:00

  • A plant outer mitochondrial membrane protein with high amino acid sequence identity to a chloroplast protein import receptor.

    abstract::We have identified a novel protein on the outer membrane of Arabidopsis thaliana mitochondria. This protein displays 67% sequence identity with the 64 kDa translocase of the outer envelope membrane of chloroplasts (Toc). A mitochondrial localisation for this protein was determined by (i). its presence in the proteome ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chew O,Lister R,Qbadou S,Heazlewood JL,Soll J,Schleiff E,Millar AH,Whelan J

    更新日期:2004-01-16 00:00:00

  • Chemical grafting of functional NAD in the active site of a dehydrogenase: regeneration in situ.

    abstract::A functional NAD molecule was immobilized at the active site of Alcohol dehydrogenase within a proteic membrane. The presence and the functionality of the cofactor was checked by fluorescence analysis. The dehydrogenase NAD membrane does not require addition of soluble cofactor for its activity. The system represents ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Legoy MD,le Moullec JM,Thomas D

    更新日期:1978-10-15 00:00:00

  • Tomosyn guides SNARE complex formation in coordination with Munc18 and Munc13.

    abstract::As a SNARE binding protein, tomosyn has been reported to negatively regulate synaptic exocytosis via arresting syntaxin-1 and SNAP-25 into a nonfusogenic product that precludes synaptobrevin-2 entry, raising the question how the assembly of the SNARE complex is achieved. Here, we have investigated new functions of tom...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Li Y,Wang S,Li T,Zhu L,Ma C

    更新日期:2018-04-01 00:00:00

  • Transient high-affinity binding of agonists to alpha 1-adrenergic receptors of intact liver cells.

    abstract::At alpha 1-adrenergic receptors in isolated rat liver parenchymal cells, (-)-epinephrine is potent in eliciting a maximal increase in glycogenolysis (Kact = 24 nM). This contrasts with a 100-fold lower affinity for the agonist at alpha 1-adrenergic receptors of intact hepatocytes determined from equilibrium competitio...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schwarz KR,Lanier SM,Carter EA,Graham RM,Homcy CJ

    更新日期:1985-08-05 00:00:00

  • Effect of glucagon on insulin receptor substrate-1 (IRS-1) phosphorylation and association with phosphatidylinositol 3-kinase (PI 3-kinase).

    abstract::In the present study we have examined the levels and phosphorylation state of the insulin receptor and insulin receptor substrate 1 (IRS-1) as well as the association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the liver and muscle of rats treated with glucagon. There was a decrease in the insulin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saad MJ,Hartmann LG,de Carvalho DS,Galoro CA,Brenelli SL,Carvalho CR

    更新日期:1995-08-14 00:00:00

  • Arsenate-mediated G2 cell cycle arrest in U-2OS cells involves phosphorylation of human polycomb protein 2 by p38 MAPK.

    abstract::G2/M checkpoints ensure the proper timing of cell mitosis. We previously reported that p38 mitogen-activated protein kinase (MAPK) activation is essential for stress-induced G2 arrest in the U-2OS osteosarcoma cell line, but the molecular mechanism was obscure. Here, using the T7 phage display system, we find p38 dire...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wu W,Zhou H,He F,Xiao Z,Jiang Y,Zhao M

    更新日期:2018-12-01 00:00:00

  • Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region.

    abstract::We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olsen J,Sjöström H,Norén O

    更新日期:1989-07-17 00:00:00