Abstract:
:ASP is a serine protease secreted by Aeromonas sobria, a sepsis-causing bacterium, and induces sepsis-mimicking disorders through plasma protein cleavage. The pathogen also secretes nASP that has a nick in the carboxy-terminal region. Compared with single-chain ASP (sASP), nASP had near-equivalent activity for small peptide substrates but was less proteolytic. Surprisingly, nASP cleaved proteins more in plasma and was inhibited by human α(2)-macroglobulin more slowly than sASP. Retarded inhibition by α(2)-macroglobulin allows nASP to keep proteolytic activity for longer in the host and exacerbate disorders at Aeromonas sobria infection sites. nASP may be an evolutional form to augment ASP virulence.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Murakami Y,Wada Y,Kobayashi H,Hasegawa M,Okamoto K,Eto M,Imamura Tdoi
10.1016/j.febslet.2012.08.004subject
Has Abstractpub_date
2012-10-19 00:00:00pages
3613-7issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00661-8journal_volume
586pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8-week high-fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respiromet...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.013
更新日期:2008-02-20 00:00:00
abstract::Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.113
更新日期:2005-02-14 00:00:00
abstract::In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81232-8
更新日期:1988-08-01 00:00:00
abstract::The effects of temperature on the gamma-aminobutyric acid (GABA) uptake and on the presteady-state and transport-associated currents of the GABA cotransporter, rat gamma-aminobutyric acid transporter 1 (rGAT1), have been studied using heterologous oocyte expression and voltage-clamp. Increasing temperature from 15 to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02271-8
更新日期:2002-02-13 00:00:00
abstract::As yet, the physiological significance of hydroxylation of anandamide and linoleoyl amides is unknown. Therefore, we investigated whether hydroxylation of ODNHEtOH and ODNH2 influences their binding abilities to the CB-1 receptor and whether it alters their reactivity towards a fatty acid amide hydrolase (FAAH) from r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01148-4
更新日期:1997-10-06 00:00:00
abstract::Iron dependent regulator, IdeR, regulates the expression of genes in response to intracellular iron levels in M. tuberculosis. Orthologs of IdeR are present in all the sequenced genomes of mycobacteria. We have used a computational approach to identify conserved IdeR regulated genes across the mycobacteria and the gen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.090
更新日期:2006-05-15 00:00:00
abstract::The main immunogenic region (MIR) of the acetylcholine receptor (AChR) is the target for the majority of high-affinity autoantibodies produced in myasthenia gravis patients. Some monoclonal antibodies (mAbs) to the MIR bind specifically, but with low affinity, to synthetic AChR alpha subunit peptides with the sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80286-r
更新日期:1990-07-02 00:00:00
abstract::The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seve...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.04.022
更新日期:2015-05-22 00:00:00
abstract::A human insulinoma cDNA (PC2) that encodes a protein homologous to the Kex2/subtilisin-like proteinases has recently been described [1990, J. Biol. Chem. 265, 2997-3000]. In order to characterise the associated proteinase activity, mRNA encoding PC2 was synthesised in vitro and microinjected into Xenopus oocytes. The ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80703-6
更新日期:1991-06-24 00:00:00
abstract::To study Bax-induced release of cytochrome c in vivo, we have expressed a cytochrome c-GFP (green fluorescent protein) fusion in Saccharomyces cerevisiae cells null for the expression of the endogenous cytochrome. We show here that cytochrome c-GFP is efficiently localised to mitochondria and able to function as an el...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01404-6
更新日期:2000-04-14 00:00:00
abstract::In the lysosomal storage disorder Gaucher disease (GD), glucosylceramide (GlcCer) accumulates due to the defective activity of glucocerebrosidase. A subset of GD patients develops neuropathology. We now show mislocalization of Limp2-positive puncta and a large reduction in the number of Lamp1-positive puncta, which ar...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12591
更新日期:2017-03-01 00:00:00
abstract::Tumour necrosis factor-alpha (TNFalpha) is a multifunctional cytokine that exerts a myriad of biological actions in numerous different tissues including adipocytes through its two distinct cell surface receptors. To address the role of each TNF receptor in the biological actions of TNFalpha in adipocytes, we have deve...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01250-3
更新日期:2000-03-03 00:00:00
abstract::Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00021-0
更新日期:1998-01-30 00:00:00
abstract::The amino acid sequence and part of the DNA sequence of a glutamic acid-specific serine protease from Streptomyces griseus is reported. This protease is shown to be homologous with other serine proteases. An improved purification protocol for this enzyme is described. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80859-2
更新日期:1991-11-04 00:00:00
abstract::Comparative allelotyping of the short arm of human chromosome 3 (3p) in four types of epithelial carcinomas was performed using an identical set of polymorphic markers. In total, 117 samples of non-papillary renal cell carcinoma (RCC), non-small cell lung carcinoma (NSCLC), carcinoma of uterine cervix (CC), and breast...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00807-8
更新日期:1999-07-09 00:00:00
abstract::Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02274-3
更新日期:2002-02-13 00:00:00
abstract::The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80643-u
更新日期:1992-06-22 00:00:00
abstract::In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.06.024
更新日期:2012-07-30 00:00:00
abstract::Unlike the human blood group Sd(a+) Tamm-Horsfall glycoprotein (THGP), the Sd(a-) one lacks terminal GalNAcbeta1--> residues at the nonreducing ends. The binding properties of this glycoprotein and its asialo product with lectins were characterized by quantitative precipitin (QPA) and precipitin inhibition assays. Amo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00320-1
更新日期:1996-04-22 00:00:00
abstract::Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Ju...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00190-8
更新日期:1999-03-05 00:00:00
abstract::We previously have shown that the overexpression of phospholipase C-gamma1 (PLC-gamma1) in rat 3Y1 fibroblasts results in malignant transformation (Chang, J.-S., Noh, D.Y., Park, I.A., Kim, M;.J., Song, H., Ryu, S.H. and Suh, P.-G. (1997) Cancer Res. 57, 5465-5468). The transformed cells, which initially are in an elo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01338-1
更新日期:1999-10-22 00:00:00
abstract::A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00280-x
更新日期:1999-03-26 00:00:00
abstract::The fluorescence intensity of the Ca2+,Mg2(+)-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 microM calcium. The observed enhancement indicates that the fluorophore, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80021-a
更新日期:1990-01-01 00:00:00
abstract::MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF form...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80078-4
更新日期:1988-08-29 00:00:00
abstract::Metal-binding peptides in proteolytic digest maps have been identified by matrix-assisted UV laser desorption time-of-flight mass spectrometry (LDTOF-MS). The plasma and milk metal transport protein chosen to demonstrate this process, histidine-rich glycoprotein (HRG), was purified and then digested with trypsin; the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80412-a
更新日期:1992-01-13 00:00:00
abstract::Actin filaments copolymerized with both intact and chemically modified actin monomers restored their sliding activity when they were supplemented with tropomyosin extracted from skeletal muscle. In contrast, the ATPase activation of the copolymers was decreased when supplemented with tropomyosin. The results indicate ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00218-9
更新日期:1996-03-25 00:00:00
abstract::The PDE4 catalytic machinery comprises, in part, two divalent cations in a binuclear motif. Here we report that PDE4A4 expressed in Sf9 cells exhibits a biphasic Mg(2+) dose-response (EC(50) of 0.15 and >10 mM) in catalyzing cAMP hydrolysis. In vitro phosphorylation of PDE4A4 by the PKA-catalytic subunit increases the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02259-7
更新日期:2002-02-13 00:00:00
abstract::Free and membrane-bound polyribosomes were isolated from the livers of untreated and clofibrate-treated male C57B1/6 mice. The in vitro translation products were investigated in a rabbit reticulocyte cell-free system by immunoprecipitation of cytosolic epoxide hydrolase, catalase and albumin. The soluble forms of epox...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80021-3
更新日期:1989-05-22 00:00:00
abstract::The plasma membrane (PM) sphingolipid composition is the result of a series of well-known metabolic pathways comprising neobiosynthesis in the endoplasmic reticulum and in the Golgi apparatus followed by vesicular delivery to the plasma membrane, membrane turnover with final catabolism in lysosomes, and shedding of me...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.11.020
更新日期:2010-05-03 00:00:00
abstract::Using radiolabeled specific cDNA glutamine synthetase mRNA could be detected by in situ hybridization exclusively within those few perivenous hepatocytes which stained immunocytochemically for glutamine synthetase. This localization of glutamine synthetase mRNA was recently reported by Moorman et al. [(1988) J. Histoc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81037-8
更新日期:1988-12-05 00:00:00