Dual role of tropomyosin on chemically modified actin filaments from skeletal muscle.


:Actin filaments copolymerized with both intact and chemically modified actin monomers restored their sliding activity when they were supplemented with tropomyosin extracted from skeletal muscle. In contrast, the ATPase activation of the copolymers was decreased when supplemented with tropomyosin. The results indicate that tropomyosin along with actin monomers may facilitate sliding activity of the entire actin filament but suppress ATPase activation of intact actin monomers themselves. Accordingly, tropomyosin molecules could be viewed as playing a dual role of both mechanical and chemical regulation of actin monomers.




FEBS letters


Honda H,Kitano Y,Hatori K,Matsuno K




Has Abstract


1996-03-25 00:00:00












  • The role of protein glycosylation in the control of cellular N-sialyltransferase activity.

    abstract::Protein glycosylation, which is a key post-translational event, is catalysed by the glycosyltransferase family of enzymes. There is an increasing body of evidence to suggest that these enzymes may themselves be glycosylated, possibly as an autocatalytic event. Using a novel in vitro system, we have investigated the ro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Breen KC

    更新日期:2002-04-24 00:00:00

  • Purification of a pituitary polypeptide that stimulates the replication of adipocyte precursors in culture.

    abstract::A bovine anterior pituitary polypeptide that stimulates the replication of rat and human adipocyte precursors has been purified. Its Mr is 44 000-53 000 and its isoelectric point is 9.8-10.3. While pituitary basic fibroblast growth factor is equally mitogenic on adipocyte precursors and skin fibroblasts, the polypepti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lau DC,Roncari DA,Yip DK,Kindler S,Nilsen SG

    更新日期:1983-03-21 00:00:00

  • p53-dependent transcriptional regulation of EDA2R and its involvement in chemotherapy-induced hair loss.

    abstract::The p53 tumor suppressor coordinates a multitude of cellular and organismal processes and exerts its activities mainly by activation of gene transcription. Here we describe the transcriptional activation of ectodysplasin A2 receptor (EDA2R) by p53 in a variety of cell types and tissues. We demonstrate that treatment o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brosh R,Sarig R,Natan EB,Molchadsky A,Madar S,Bornstein C,Buganim Y,Shapira T,Goldfinger N,Paus R,Rotter V

    更新日期:2010-06-03 00:00:00

  • Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line.

    abstract::We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

    更新日期:2001-11-23 00:00:00

  • A comparison and analysis of the toxicity and receptor binding properties of Bacillus thuringiensis CryIC delta-endotoxin on Spodoptera littoralis and Bombyx mori.

    abstract::The binding of L-[35S]methionine in vivo labelled CryIC toxin to its receptor in brush border membrane vesicle (BBMV's) prepared from Spodoptera littoralis and Bombyx mori was studied. Both insect species were highly susceptible to the CryIC toxin in bioassays, B. mori being 7-fold more sensitive to CryIC than S. litt...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sanchis V,Chaufaux J,Pauron D

    更新日期:1994-10-24 00:00:00

  • NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.

    abstract::Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rentsch D,Laloi M,Rouhara I,Schmelzer E,Delrot S,Frommer WB

    更新日期:1995-08-21 00:00:00

  • Phosphoglycerate mutase in mammalian striated muscles: subcellular localization and binding partners.

    abstract::Contrary to previously published data, we have found that in mammalian skeletal muscles, phosphoglycerate mutase (PGM) is organized in a regular, striated fashion within the sarcomere. In the absence of the enzyme effectors, PGM localizes mainly at the M-line, but under conditions typical for contracting muscle, the e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kowalski W,Gizak A,Rakus D

    更新日期:2009-06-18 00:00:00

  • Mechanism and site of action of a ribosome-inactivating protein type 1 from Dianthus barbatus which inactivates Escherichia coli ribosomes.

    abstract::A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Prestle J,Hornung E,Schönfelder M,Mundry KW

    更新日期:1992-02-10 00:00:00

  • Structural features in aminoacyl-tRNAs required for recognition by elongation factor Tu.

    abstract::In bacterial polypeptide synthesis aminoacyl-tRNA (aa-tRNA) bound to elongation factor Tu (EF-Tu) and GTP is part of a crucial intermediate ribonucleoprotein complex involved in the decoding of messenger RNA. The conformation and topology as well as the affinity of the macromolecules in this ternary aa-tRNA X EF-Tu X ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Faulhammer HG,Joshi RL

    更新日期:1987-06-15 00:00:00

  • Cloning of a human liver UDP-glucose pyrophosphorylase cDNA by complementation of the bacterial galU mutation.

    abstract::A human liver cDNA clone which encodes the UDP-glucose pyrophosphorylase was isolated by complementation of a bacterial galU mutant. The deduced amino acid sequence of the human enzyme comprised 508 amino acids with a calculated molecular mass of 56,950. The human enzyme significantly resembles those of potato tuber a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peng HL,Chang HY

    更新日期:1993-08-23 00:00:00

  • Cyclin B1 transcription is enhanced by the p300 coactivator and regulated during the cell cycle by a CHR-dependent repression mechanism.

    abstract::Cyclin B is a central regulator of transition from the G(2) phase of the cell cycle to mitosis. In mammalian cells two B-type cyclins have been characterised, cyclin B1 and B2. Both are expressed with a maximum in G(2) and their synthesis is mainly regulated on the transcriptional level. We show that a single cell cyc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wasner M,Tschöp K,Spiesbach K,Haugwitz U,Johne C,Mössner J,Mantovani R,Engeland K

    更新日期:2003-02-11 00:00:00

  • Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.

    abstract::Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

    更新日期:2018-04-01 00:00:00

  • Polyunsaturated fatty acids potentiate interleukin-1-stimulated arachidonic acid release by cells overexpressing type IIA secretory phospholipase A2.

    abstract::By analyzing human embryonic kidney 293 cell transfectants stably overexpressing various types of phospholipase A2 (PLA2), we have shown that polyunsaturated fatty acids (PUFAs) preferentially activate type IIA secretory PLA2 (sPLA2-IIA)-mediated arachidonic acid (AA) release from interleukin-1 (IL-1)-stimulated cells...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kambe T,Murakami M,Kudo I

    更新日期:1999-06-18 00:00:00

  • Hsp70 inhibits lipopolysaccharide-induced NF-kappaB activation by interacting with TRAF6 and inhibiting its ubiquitination.

    abstract::Inducible heat shock protein 70 (Hsp70) is one of the most important HSPs for maintenance of cell integrity during normal cellular growth as well as pathophysiological conditions. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial signaling transducer that regulates a diverse array of physiologica...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chen H,Wu Y,Zhang Y,Jin L,Luo L,Xue B,Lu C,Zhang X,Yin Z

    更新日期:2006-05-29 00:00:00

  • Guanylate cyclase in rod outer segments of the toad retina. Effect of light and Ca2+.

    abstract::Guanylate cyclase activity was measured in disrupted rod outer segments of the toad retina. The experiments showed that cGMP is synthesized from GTP at a rate of 3 +/- 1 nmol/min per mg protein. In darkness this value is largely independent of the Ca2+ concentration, while it is enhanced by flashes of light of increas...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pepe IM,Panfoli I,Cugnoli C

    更新日期:1986-07-14 00:00:00

  • Mutation of an evolutionarily conserved tyrosine residue in the active site of a human class Alpha glutathione transferase.

    abstract::Human class Alpha glutathione transferase (GST) A1-1 has been subjected to site-directed mutagenesis of a Tyr residue conserved in all classes of cytosolic GSTs. The change of Tyr8----Phe lowers the specific activities with three substrates to 2-8% of the values for the wild-type enzyme. The changes in the kinetic par...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stenberg G,Board PG,Mannervik B

    更新日期:1991-11-18 00:00:00

  • Kinetic mechanism of pyruvate decarboxylase. Evidence for a specific protonation of the enzymic intermediate.

    abstract::Decarboxylation of pyruvate by pyruvate decarboxylase (EC was performed in a reaction mixture containing 50% deuterium. The isolated product, acetaldehyde, was investigated directly by 1H NMR and by mass spectrometry after conversion to the 2,4-dinitrophenyl hydrazone. The protium content of 56% at acetaldehy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ermer J,Schellenberger A,Hübner G

    更新日期:1992-03-09 00:00:00

  • The influence of oligonucleotide-effector on the selectivity of sequence specific modification of 16 S rRNA.

    abstract::The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constan...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zenkova MA,Fedorova OS,Levina AS,Mamaev SV,Karpova GG

    更新日期:1990-08-20 00:00:00

  • Using lipoate enantiomers and thioredoxin to study the mechanism of the 2-oxoacid-dependent dihydrolipoate production by the 2-oxoacid dehydrogenase complexes.

    abstract::The thioredoxin-catalyzed insulin reduction by dihydrolipoate was applied to study the 2-oxoacid: lipoate oxidoreductase activity of 2-oxoacid dehydrogenase complexes. The enzymatic and non-enzymatic mechanisms of the transfer of reducing equivalents from the complexes to free lipoic acid (alpha-lipoic acid, 6,8-thioo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bunik V,Shoubnikova A,Loeffelhardt S,Bisswanger H,Borbe HO,Follmann H

    更新日期:1995-09-04 00:00:00

  • Pharmacological targeting of α-synuclein and TPPP/p25 in Parkinson's disease: challenges and opportunities in a Nutshell.

    abstract::With the aging of population, neurological disorders, and especially disorders involving defects in protein conformation (also known as proteopathies) pose a serious socio-economic problem. So far there is no effective treatment for most proteopathies, including Parkinson's disease (PD). The mechanism underlying PD pa...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Oláh J,Ovádi J

    更新日期:2019-07-01 00:00:00

  • Evidence for a hydroxy-aluminium polymer (Al13) in synaptosomes.

    abstract::The presence of the hydroxy-aluminium polymer (Al13-(OH)24O4(H2O)12(7+)) was noticed inside synaptosomes when synaptosomes were incubated with Al(NO3)3 at neutral pH values. Dysprosium nitrate (Dy(NO3)3)--a shift reagent--facilities the identification of the Al13 species distinctly inside the synaptosomes. 27Al NMR wa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rao GV,Rao KS

    更新日期:1992-10-12 00:00:00

  • Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins.

    abstract::Human kallikrein 8 (KLK8) is a member of the human kallikrein gene family of serine proteases, and its protein, hK8, has recently been suggested to serve as a new ovarian cancer marker. To gain insights into the physiological role of hK8, the active recombinant enzyme was obtained in a pure state for biochemical and e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rajapakse S,Ogiwara K,Takano N,Moriyama A,Takahashi T

    更新日期:2005-12-19 00:00:00

  • Abnormally phosphorylated tau in SY5Y human neuroblastoma cells.

    abstract::In Alzheimer disease (AD) the microtubule associated protein (MAP) tau is hyperphosphorylated at several sites. In the present study, like AD tau, tau in the human neuroblastoma SH-SY5Y was found to be hyperphosphorylated, at Ser-199/202, Thr-231, Ser-396 and Ser-404. However, in contrast to AD, the tau in SY5Y cells ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tanaka T,Iqbal K,Trenkner E,Liu DJ,Grundke-Iqbal I

    更新日期:1995-02-20 00:00:00

  • Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and codes for a sperm motility inhibitor protein.

    abstract::Boar semen contains a seminal plasma motility inhibitor (SPMI) that blocks the motility of demembranated-reactivated spermatozoa as well as of intact spermatozoa. In this paper, we describe the primary structure of SPMI, the coding of boar SPMI cDNA gene and its expression in various porcine tissues. Nucleotide sequen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iwamoto T,Hiroaki H,Furuichi Y,Wada K,Satoh M,Satoh M,Osada T,Gagnon C

    更新日期:1995-07-24 00:00:00

  • Induction of tcI 7, a gene encoding a beta-subunit of proteasome, in tobacco plants treated with elicitins, salicylic acid or hydrogen peroxide.

    abstract::We previously isolated, by differential display and 5' RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for tobacco cryptogein Induced), a gene encoding a beta-subunit of proteasome. Here, we report that tc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Etienne P,Petitot AS,Houot V,Blein JP,Suty L

    更新日期:2000-01-28 00:00:00

  • The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter.

    abstract::The cDNA coding for the inducible H+/hexose cotransporter of Chlorella kessleri has been cloned and sequenced. It was isolated by differential screening of a cDNA library prepared from glucose-induced cells. The increase in expression of the gene correlates quantitatively with the increase in uptake activity due to in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sauer N,Tanner W

    更新日期:1989-12-18 00:00:00

  • Transient expression of botulinum neurotoxin C1 light chain differentially inhibits calcium and glucose induced insulin secretion in clonal beta-cells.

    abstract::We have investigated the effect of botulinum neurotoxin (BoNT) C1 light chain (LC) on insulin exocytosis from the clonal beta-cell line HIT-T15. In streptolysin-O permeabilized cells, the beta-cell impermeant BoNT C1 cleaved mainly syntaxin 1 and inhibited Ca2+ as well as GTPgammaS induced exocytosis. To study the eff...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Land J,Zhang H,Vaidyanathan VV,Sadoul K,Niemann H,Wollheim CB

    更新日期:1997-12-08 00:00:00

  • Trypanosomatid hydrogen peroxide [corrected] metabolism.

    abstract::The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Penketh PG,Kennedy WP,Patton CL,Sartorelli AC

    更新日期:1987-09-14 00:00:00

  • The effect of hydroxylation of linoleoyl amides on their cannabinomimetic properties.

    abstract::As yet, the physiological significance of hydroxylation of anandamide and linoleoyl amides is unknown. Therefore, we investigated whether hydroxylation of ODNHEtOH and ODNH2 influences their binding abilities to the CB-1 receptor and whether it alters their reactivity towards a fatty acid amide hydrolase (FAAH) from r...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van der Stelt M,Paoletti AM,Maccarrone M,Nieuwenhuizen WF,Bagetta G,Veldink GA,Finazzi Agrò A,Vliegenthart JF

    更新日期:1997-10-06 00:00:00

  • Mapping of beta-adrenoceptor coupling domains to Gs-protein by site-specific synthetic peptides.

    abstract::Peptides corresponding to the known sequence of turkey erythrocyte beta 1-adrenergic receptor were synthesized and the effects on receptor-mediated cyclase activation were measured. Peptides corresponding to the first and second intracellular loops (T61-71 and T138-159) inhibited at micromolar concentrations the hormo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Palm D,Münch G,Dees C,Hekman M

    更新日期:1989-08-28 00:00:00