Dual role of tropomyosin on chemically modified actin filaments from skeletal muscle.


:Actin filaments copolymerized with both intact and chemically modified actin monomers restored their sliding activity when they were supplemented with tropomyosin extracted from skeletal muscle. In contrast, the ATPase activation of the copolymers was decreased when supplemented with tropomyosin. The results indicate that tropomyosin along with actin monomers may facilitate sliding activity of the entire actin filament but suppress ATPase activation of intact actin monomers themselves. Accordingly, tropomyosin molecules could be viewed as playing a dual role of both mechanical and chemical regulation of actin monomers.




FEBS letters


Honda H,Kitano Y,Hatori K,Matsuno K




Has Abstract


1996-03-25 00:00:00












  • 2-Cyclopenten-1-one and prostaglandin J2 reduce restenosis after balloon angioplasty in rats: role of NF-kappaB.

    abstract::The aim of this study was to evaluate, using a rat model of balloon angioplasty, whether prostaglandin (PG) J(2) and 2-cyclopenten-1-one are able to reduce restenosis. We found that both PGJ(2) and 2-cyclopenten-1-one, administered by local application on carotid arteries, caused a dose-dependent inhibition of neointi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ianaro A,Maffia P,Cuzzocrea S,Mazzon E,Santoro MG,Di Rosa M,Ialenti A

    更新日期:2003-10-09 00:00:00

  • Functional identification of AtFao3, a membrane bound long chain alcohol oxidase in Arabidopsis thaliana.

    abstract::The Arabidopsis thalina genome database was searched for homologues of the Candida cloacae fao1 gene which encodes a membrane bound, flavin-containing, hydrogen peroxide generating, long chain alcohol oxidase. This gene has not been isolated from plants or animals. Four putative candidates were found in the database b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cheng Q,Liu HT,Bombelli P,Smith A,Slabas AR

    更新日期:2004-09-10 00:00:00

  • Cdx2 homeodomain protein regulates the expression of MOK, a member of the mitogen-activated protein kinase superfamily, in the intestinal epithelial cells.

    abstract::Regulatory protein kinases are involved in various cellular processes such as proliferation, differentiation, and apoptosis. Using cDNA differential display, we identified MOK, a member of the mitogen-activated protein kinase superfamily, as one of the genes induced by a caudal-related homeobox transcription factor, C...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Uesaka T,Kageyama N

    更新日期:2004-08-27 00:00:00

  • Hepatotropin mRNA expression in human foetal liver development and in liver regeneration.

    abstract::A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Selden C,Jones M,Wade D,Hodgson H

    更新日期:1990-09-17 00:00:00

  • Crystallization of the cpn60/cpn10 complex ('holo-chaperonin') from Thermus thermophilus.

    abstract::A stable complex of the chaperonins, cpn60 and cpn10 (Escherichia coli GroEL and GroES homologues), from the extremely thermophilic bacterium Thermus thermophilus has been isolated and crystallized. The crystals have dimensions up to 30 x 200 x 200 microns. Ultra-thin sections of the crystals estimated by electron mic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lissin NM,Sedelnikova SE,Ryazantsev SN

    更新日期:1992-10-12 00:00:00

  • A single d(GpG) cisplatin adduct on the estrogen response element decreases the binding of the estrogen receptor.

    abstract::Both cisplatin and the estrogen receptor (ER) are known to bend DNA. The influence of the bending of sequences by the d(GpG)cisPt adduct binding of ER to estrogen response element (ERE)-like sequences was examined. Three ERE-like oligonucleotides with different affinities for ER and which include a GG in the linker se...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Massaad-Massade L,Massaad C,Legendre F,Bas V,Chottard J,Beaune P,Barouki R

    更新日期:2000-01-21 00:00:00

  • Regulatory T-cells in systemic lupus erythematosus and rheumatoid arthritis.

    abstract::Regulatory T-cells (Tregs) are the guardians of peripheral tolerance acting to prevent autoimmune diseases such as systemic lupus erythomatosus (SLE) and rheumatoid arthritis (RA). Defects in Tregs have been reported in these two diseases despite significant differences in their clinical phenotype and pathogenesis. In...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Chavele KM,Ehrenstein MR

    更新日期:2011-12-01 00:00:00

  • Differential expression of the protein kinase A regulatory subunit (RIalpha) in pancreatic endocrine cells.

    abstract::A PCR-based subtractive cloning procedure was used to identify genes expressed at higher levels in the pancreatic beta cell line betaTC1, as compared to the pancreatic alpha cell line alphaTC1. One of the clones isolated by this procedure corresponded to the regulatory subunit (RIalpha) of protein kinase A (PKA). Usin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Arava Y,Adamsky K,Belleli A,Shaltiel S,Walker MD

    更新日期:1998-03-20 00:00:00

  • Characterization of Arabidopsis decapping proteins AtDCP1 and AtDCP2, which are essential for post-embryonic development.

    abstract::Although decapping is an important process in eukaryotic mRNA turnover, little is known about this process in plants. Here, we identified Arabidopsis thaliana decapping proteins AtDCP1 and AtDCP2 and showed that (I) AtDCP2 is an active decapping enzyme, (II) AtDCP1 interacts with itself, (III) AtDCP1 and AtDCP2 are lo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iwasaki S,Takeda A,Motose H,Watanabe Y

    更新日期:2007-05-29 00:00:00

  • Differential cytokine modulation of the genes LAMA3, LAMB3, and LAMC2, encoding the constitutive polypeptides, alpha 3, beta 3, and gamma 2, of human laminin 5 in epidermal keratinocytes.

    abstract::Laminin 5, an anchoring filament protein previously known as nicein/kalinin/epiligrin, consists of three polypeptide chains, alpha 3, beta 3, and gamma 2, encoded by the genes LAMA3, LAMB3, and LAMC2, respectively. The expression of laminin 5 was detected by Northern hybridization with specific cDNA probes in various ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Korang K,Christiano AM,Uitto J,Mauviel A

    更新日期:1995-07-24 00:00:00

  • Laser Raman studies of the 5 S rRNA-protein L5 complex of rat liver ribosomes.

    abstract::The effects of ribosomal protein L5 on the conformation of 5 S rRNA in the 5 S rRNA-protein L5 complex extracted from rat liver ribosomes have been studied by laser Raman spectroscopy. A comparison of the spectra shows small protein-induced conformational changes in the 5 S rRNA, but most of the base-paired regions ap...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fabian H,Böhm S,Carius W,Misselwitz R,Welfle H

    更新日期:1983-05-08 00:00:00

  • Purification and functional characterization of beta-adrenergic receptor kinase expressed in insect cells.

    abstract::The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation of beta-adrenergic receptors, which is thought to represent the first step of homologous desensitization. We have expressed bovine and human beta ARK1 in Sf9 cells and purified them to apparent homogeneity in milligram quantities. The Km-va...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Söhlemann P,Hekman M,Buchen C,Elce JS,Lohse MJ

    更新日期:1993-06-07 00:00:00

  • Regulation of Rab5 isoforms by transcriptional and post-transcriptional mechanisms in yeast.

    abstract::Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins: Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels incre...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Schmidt O,Weyer Y,Fink MJ,Müller M,Weys S,Bindreither M,Teis D

    更新日期:2017-09-01 00:00:00

  • 31P-NMR studies of Mycoplasma gallisepticum cells using a continuous perfusion technique.

    abstract::31P-NMR studies of Mycoplasma gallisepticum cells have been carried out using a continuous perfusion technique; these are the first such studies with this organism. Using this technique, glucose metabolism was monitored in the intact organisms, and cell extracts were prepared to identify the intermediates. Under glyco...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Egan W,Barile M,Rottem S

    更新日期:1986-08-18 00:00:00

  • Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal.

    abstract::PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang Z,Takemori H,Halder SK,Nonaka Y,Okamoto M

    更新日期:1999-06-18 00:00:00

  • Tissue-specific regulation of the expression of rat intestinal bile acid-binding protein.

    abstract::A lipid-binding protein identical to the rat intestinal bile acid-binding protein, termed I-15P, was expressed in steroid hormone-producing tissues such as ovary and adrenal gland, but not testis. In immature rats, I-15P was expressed in intestine but not in ovaries. The expression of I-15P in the ovaries of immature ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato E,Fujii H,Fujita M,Kanda T,Iseki S,Hatakeyama K,Tanaka T,Ono T

    更新日期:1995-10-30 00:00:00

  • Insulin-dependent translocation of the small GTP-binding protein rab3C in cardiac muscle: studies on insulin-resistant Zucker rats.

    abstract::The failure of insulin-regulated recruitment of the GLUT4 glucose transporter in cardiac muscle of obese Zucker rats is associated with alterations of the subcellular distribution of the small-molecular-mass GTP-binding protein rab4A. Here, we show by subcellular fractionation and Western blotting a translocation of t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Uphues I,Chern Y,Eckel J

    更新日期:1995-12-18 00:00:00

  • Effects of analogues of adenosine and methyl xanthines on insulin sensitivity in soleus muscle of the rat.

    abstract::The concentration of insulin that produces half-maximal stimulation of glycolysis by stripped soleus muscle preparations is markedly increased by the adenosine analogues, 2-chloroadenosine and N6-phenylisopropyladenosine, but is markedly decreased by the methyl xanthine analogue, 8-phenyltheophylline. 2-Chloroadenosin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Budohoski L,Challiss RA,McManus B,Newsholme EA

    更新日期:1984-02-13 00:00:00

  • Crystallization and preliminary crystallographic studies of recombinant dimerization cofactor of transcription factor HNF1/pterin-4 alpha-carbinolamine dehydratase from liver.

    abstract::The bi-functional protein dimerization cofactor of HNF1 (DCoH)/pterin-4 alpha-carbinolamine dehydratase (PCD) is found in liver cell nuclei bound to the transcription factor hepatocyte nuclear factor 1 (HNF1) as well as in the cytoplasm acting as an enzyme involved in the phenylalanine hydroxylation system. Deficiency...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ficner R,Sauer UH,Ceska TA,Stier G,Suck D

    更新日期:1995-01-02 00:00:00

  • Identification of a novel Skp2-like mammalian protein containing F-box and leucine-rich repeats.

    abstract::The F-box protein Skp2 is important for S phase entry and binds to Skp1 and the cyclin A-Cdk2 complex. Here we report the cloning, analysis of genomic organization and characterization of a novel gene product related to Skp2 named FBL2. The human FBL2 gene was found to be a highly interrupted gene of at least 126.6 kb...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ilyin GP,Rialland M,Glaise D,Guguen-Guillouzo C

    更新日期:1999-10-01 00:00:00

  • House dust mite allergen Der f 1 can induce the activation of latent TGF-beta via its protease activity.

    abstract::A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakamura Y,Miyata M,Shimokawa N,Ohnuma Y,Katoh R,Ogawa H,Okumura K,Nakao A

    更新日期:2009-06-18 00:00:00

  • Mitochondrial Hsp78, a member of the Clp/Hsp100 family in Saccharomyces cerevisiae, cooperates with Hsp70 in protein refolding.

    abstract::The molecular chaperone protein Hsp78, a member of the Clp/Hsp100 family localized in the mitochondria of Saccharomyces cerevisiae, is required for maintenance of mitochondrial functions under heat stress. To characterize the biochemical mechanisms of Hsp78 function, Hsp78 was purified to homogeneity and its role in t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Krzewska J,Langer T,Liberek K

    更新日期:2001-01-26 00:00:00

  • Domain interactions stabilize the alternatively folded state of an antibody Fab fragment.

    abstract::The structure of the Fab fragment of the monoclonal antibody MAK 33 (kappa/IgG1) at pH 2 was characterized. Spectroscopic and kinetic analysis revealed a molten globule-like state, characterized by elements of secondary structure but less defined tertiary contacts than in the native state. However, some aromatic side ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lilie H,Buchner J

    更新日期:1995-03-27 00:00:00

  • The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix.

    abstract::The cleavable prepiece of the precursor to yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) was attached to the amino-terminus of mouse dihydrofolate reductase (a cytosolic protein) by gene fusion. The resulting fusion protein was imported into the matrix of isolated, energized yeast mitochond...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hurt EC,Pesold-Hurt B,Schatz G

    更新日期:1984-12-10 00:00:00

  • Importance of local structures of second and third repeat fragments of microtubule-binding domain for tau filament formation.

    abstract::To investigate the importance of the seventh residue of the second and third repeat fragments (R2 and R3 peptides) of the microtubule-binding domain (MBD) for tau filamentous assembly, the residues Lys and Pro were substituted (R2-K7P and R3-P7K). The filament formations of the R2 and R3 peptides were almost lost due ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tokimasa M,Minoura K,Hiraoka S,Tomoo K,Sumida M,Taniguchi T,Ishida T

    更新日期:2005-07-04 00:00:00

  • The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa.

    abstract::The nucleotide sequence of the amiE gene, encoding the aliphatic amidase of Pseudomonas aeruginosa, has been determined. The sequence of 1038 nucleotides shows a strong bias in favour of codons with G or C in the third position, and only 44 different codons are utilised. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brammar WJ,Charles IG,Matfield M,Liu CP,Drew RE,Clarke PH

    更新日期:1987-05-11 00:00:00

  • Interaction between the Arabidopsis thaliana heat shock transcription factor HSF1 and the TATA binding protein TBP.

    abstract::The heat shock factor (HSF1) is the central regulator of the heat stress (hs) response and is required for stimulating the transcription of the hs genes and consequently the expression of heat shock proteins. To promote the polymerase II-dependent transcription of the hs genes, HSF has to communicate with the basal tr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Reindl A,Schöffl F

    更新日期:1998-10-09 00:00:00

  • Demonstration of cell-specific phosphorylation of LTC4 synthase.

    abstract::PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gupta N,Nicholson DW,Ford-Hutchinson AW

    更新日期:1999-04-16 00:00:00

  • Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif.

    abstract::The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the fir...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nguyen TN,You DJ,Kanaya E,Koga Y,Kanaya S

    更新日期:2013-05-02 00:00:00

  • pH-dependent changes in structure and RNA-binding activity of casein kinase 2 from Rana temporaria oocytes.

    abstract::It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kandror KV,Kapkov DV,Turapov OA,Stepanov AS

    更新日期:1991-06-03 00:00:00