Abstract:
:We previously have shown that the overexpression of phospholipase C-gamma1 (PLC-gamma1) in rat 3Y1 fibroblasts results in malignant transformation (Chang, J.-S., Noh, D.Y., Park, I.A., Kim, M;.J., Song, H., Ryu, S.H. and Suh, P.-G. (1997) Cancer Res. 57, 5465-5468). The transformed cells, which initially are in an elongated and flat form after seeding in plastic dishes, become rounded during continued culture. We found that tyrosine dephosphorylation of paxillin accompanies this morphological change of the transformed cells and that PLC-gamma1 co-immunoprecipitates together with paxillin and vice versa, but not after the cells have become round. Transformed cells growing on fibronectin-pre-coated dishes regain their flat morphology and this is accompanied by paxillin tyrosine phosphorylation. Furthermore, immunoprecipitation analysis showed that paxillin forms a heteromeric complex with PLC-gamma1 in cells grown on fibronectin. These results suggest that a complex formation between paxillin and PLC-gamma1 may play a role in cell-substrate adhesion.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Chang JS,Iwashita S,Lee YH,Kim MJ,Ryu SH,Suh PGdoi
10.1016/s0014-5793(99)01338-1keywords:
subject
Has Abstractpub_date
1999-10-22 00:00:00pages
161-5issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01338-1journal_volume
460pub_type
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