Abstract:
:Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase (AldO) is linked to OleTJE . AldO oxidizes polyols (including glycerol), generating H2 O2 as a coproduct and facilitating its use for efficient OleTJE -dependent fatty acid decarboxylation. AldO activity is regulatable by polyol substrate titration, enabling control over H2 O2 supply to minimize oxidative inactivation of OleTJE and prolong activity for increased alkene production. We also use these fusion systems to generate novel products from secondary turnover of 2-OH and 3-OH myristic acid primary products, expanding the catalytic repertoire of OleTJE .
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Matthews S,Tee KL,Rattray NJ,McLean KJ,Leys D,Parker DA,Blankley RT,Munro AWdoi
10.1002/1873-3468.12581subject
Has Abstractpub_date
2017-03-01 00:00:00pages
737-750issue
5eissn
0014-5793issn
1873-3468journal_volume
591pub_type
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