Abstract:
:The reaction of alpha-chymotrypsin with AcTyr-OEt and with AcTrp-OEt at pH 7.0 and 7.8 was studied over a wide range of substrate concentrations. The reaction with AcTyr-OEt at pH 7.8 was shown to be non-hyperbolic using a variety of criteria whereas those at pH 7.0 with the same substrate and at both pH values with AcTrp-OEt were hyperbolic. The non-hyperbolicity of the reaction with AcTyr-OEt at pH 7.8 followed a pattern of negative cooperativity with a Hill coefficient for the high substrate concentration range of 0.48. Although other explanations are possible, the pH dependence of the reaction with AcTyr-OEt could be related to the slow transition of the two known forms of the enzyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Garrell J,Cuchillo CMdoi
10.1016/0014-5793(85)81312-0subject
Has Abstractpub_date
1985-10-14 00:00:00pages
329-32issue
2eissn
0014-5793issn
1873-3468journal_volume
190pub_type
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