The RacGAP protein FilGAP is a negative regulator of chemokine-promoted lymphocyte migration.

abstract：:The Na-Ca exchanger is essential for the Ca2+ homeostasis in many cell types. This transporter has been difficult to investigate because no specific inhibitor is available. We have synthesized an antisense oligodeoxynucleotide directed against the rat cardiac Na-Ca exchanger mRNA. To estimate the activity of the Na-Ca...

journal_title：FEBS letters

authors： Lipp P,Schwaller B,Niggli E

更新日期：1995-05-08 00:00:00

abstract：:The state of assembly of the pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes was examined after the dihydrolipoyl acyltransferase (E2) component of each enzyme system had been subjected to varying degrees of limited proteolysis. Dissociation of the dihydrolipoyl dehydrogenase (E3) component accompanied...

journal_title：FEBS letters

authors： Packman LC,Perham RN

更新日期：1986-10-06 00:00:00

abstract：:The kinetic constants of internalization of asialoorosomucoid were determined for normal and jejuno-ileal by-passed rat hepatocytes. In by-passed rats the maximum velocity of asialoorosomucoid internalization is decreased 3-fold, without any modification of apparant constant of internalization. Moreover, the rate cons...

journal_title：FEBS letters

authors： Serbource-Goguel N,Dodeur M,Borel B,Feger J,Agneray J,Durand G

更新日期：1985-04-22 00:00:00

abstract：:The fidelity of tRNA aminoacylation is dependent in part on amino acid editing mechanisms. A hydrolytic activity that clears mischarged tRNAs typically resides in an active site on the tRNA synthetase that is distinct from its synthetic aminoacylation active site. A second pre-transfer editing pathway that hydrolyzes ...

journal_title：FEBS letters

authors： Martinis SA,Boniecki MT

更新日期：2010-01-21 00:00:00

abstract：:X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

journal_title：FEBS letters

authors： Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

更新日期：2012-06-04 00:00:00

abstract：:We counted base transitions (Ts) and transversions (Tv) in mutations among various types of hepatitis C virus (HCV) sequences. The Ts/Tv ratio was useful for distinguishing the (geno)type of the compared HCV sequences, since the ratio was 4.0 or more among sequences of the same type, while it was less than 2.0 among s...

journal_title：FEBS letters

authors： Tanaka T,Kato N,Hijikata M,Shimotohno K

更新日期：1993-01-04 00:00:00

abstract：:Monomeric and trimeric PS I complexes missing the three stromal subunits E,C and D (termed PS I core complexes) were prepared from the thermophilic cyanobacterium Synechococcus sp. by incubation with urea. The subunits E,C and D are sequentially removed. In the monomeric PS I the subunit C is removed with a half life ...

journal_title：FEBS letters

authors： Lüneberg J,Fromme P,Jekow P,Schlodder E

更新日期：1994-01-31 00:00:00

abstract：:In higher plants, two types of nitrate transporters, NRT1 and NRT2, have been identified. In Arabidopsis, there are 53 NRT1 genes and 7 NRT2 genes. NRT2 are high-affinity nitrate transporters, while most members of the NRT1 family are low-affinity nitrate transporters. The exception is CHL1 (AtNRT1.1), which is a dual...

journal_title：FEBS letters

authors： Tsay YF,Chiu CC,Tsai CB,Ho CH,Hsu PK

更新日期：2007-05-25 00:00:00

abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

journal_title：FEBS letters

authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

更新日期：2005-08-15 00:00:00

abstract：:Fatty acid acylation of membrane proteins was studied on human erythrocytes by measuring incorporation of [3H]palmitate at different specific radioactivities. A 55 kDa polypeptide within the band 4.5 region was the main acceptor protein for acylation by fatty acids (palmitate, stearate, oleate), while other polypeptid...

journal_title：FEBS letters

authors： Maretzki D,Mariani M,Lutz HU

更新日期：1990-01-01 00:00:00

abstract：:Human hepatocellular carcinoma (HCC) is one of the most common tumors worldwide, in which the genetic mechanisms of oncogenesis are still unclear. To investigate whether the genomic DNA copy number alterations may contribute to primary HCC, the cDNA microarray-based comparative genomic hybridization (CGH) analysis was...

journal_title：FEBS letters

authors： Huang J,Sheng HH,Shen T,Hu YJ,Xiao HS,Zhang Q,Zhang QH,Han ZG

更新日期：2006-06-26 00:00:00

abstract：:Activation of p53 plays a central role in the cell's response to various stress signals. We investigated whether p53 is activated upon disruption of actin microfilaments, caused by cytochalasin D (CD). We show that treatment with CD leads to accumulation of p53 in the cells and activation of p53-dependent transcriptio...

journal_title：FEBS letters

authors： Rubtsova SN,Kondratov RV,Kopnin PB,Chumakov PM,Kopnin BP,Vasiliev JM

更新日期：1998-07-03 00:00:00

abstract：:The p53 tumour suppressor protein is tightly controlled by the E3 ubiquitin ligase, mouse double minute 2 (MDM2), but maintains MDM2 expression as part of a negative feedback loop. We have identified the immunophilin, 25kDa FK506-binding protein (FKBP25), previously shown to be regulated by p53-mediated repression, as...

journal_title：FEBS letters

authors： Ochocka AM,Kampanis P,Nicol S,Allende-Vega N,Cox M,Marcar L,Milne D,Fuller-Pace F,Meek D

更新日期：2009-02-18 00:00:00

abstract：:A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) fro...

journal_title：FEBS letters

authors： Nguyen TM,Ellis JM,Ginjaar IB,van Paassen MM,van Ommen GJ,Moorman AF,Cartwright AJ,Morris GE

更新日期：1990-10-15 00:00:00

abstract：:The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...

journal_title：FEBS letters

authors： Hasan S,Osickova A,Bumba L,Novák P,Sebo P,Osicka R

更新日期：2015-01-30 00:00:00

abstract：:Activation gating in KcsA is elicited by changes in intracellular proton concentration. Thompson et al. identified a charge cluster around the inner gate that plays a key role in defining proton activation in KcsA. Here, through functional and spectroscopic approaches, we confirmed the role of this charge cluster and ...

journal_title：FEBS letters

authors： Cuello LG,Cortes DM,Jogini V,Sompornpisut A,Perozo E

更新日期：2010-03-19 00:00:00

abstract：:C26, the C-terminal 26 residue peptide of serpin A1, significantly increased cell proliferation in cultures of hepatoma cells, but not in porcine kidney epithelial cells, human skin fibroblasts or keratinocytes. The mitogenic activity of C26 was preferentially inhibited with a protein kinase C (PKC) inhibitor, an anti...

journal_title：FEBS letters

authors： Congote LF,Temmel N

更新日期：2004-10-22 00:00:00

abstract：:Intrinsically disordered proteins participate in many important cellular regulatory processes. The absence of a well-defined structure in the free state of a disordered domain, and even on occasion when it is bound to physiological partners, is fundamental to its function. Disordered domains are frequently the locatio...

journal_title：FEBS letters

authors： Berlow RB,Dyson HJ,Wright PE

更新日期：2015-09-14 00:00:00

abstract：:An E. coli overexpression system for the N-terminal domain of the 434 repressor with residues 1-63 (434 repressor(1-63)) was constructed and used to produce this polypeptide with uniform 15N-labeling, and with 13C-labeling of the methyl groups of valine and leucine. Using these protein preparations almost complete seq...

journal_title：FEBS letters

authors： Neri D,Wider G,Wüthrich K

更新日期：1992-06-01 00:00:00

abstract：:Cellular ageing is characterized by the accumulation of oxidatively modified proteins which may be due to increased protein damage and/or decreased elimination of oxidized protein. Since the proteasome is in charge of protein turnover and removal of oxidized protein, its fate during ageing and upon oxidative stress ha...

journal_title：FEBS letters

authors： Friguet B

更新日期：2006-05-22 00:00:00

abstract：:Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lack...

journal_title：FEBS letters

authors： Abe Y,Goda W,Ikeshima-Kataoka H,Yasui M

更新日期：2017-12-01 00:00:00

abstract：:We have investigated the functional importance of nucleotide sequence differences between proximal (P-) and distal (D-) p53-binding elements in the MCK promoter. P- and D-elements normally co-operate to permit synergistic promoter activation by p53. Interestingly, we find that P-elements cannot co-operate with each ot...

journal_title：FEBS letters

authors： Jackson P,Yardley G

更新日期：1997-04-14 00:00:00

abstract：:At alpha 1-adrenergic receptors in isolated rat liver parenchymal cells, (-)-epinephrine is potent in eliciting a maximal increase in glycogenolysis (Kact = 24 nM). This contrasts with a 100-fold lower affinity for the agonist at alpha 1-adrenergic receptors of intact hepatocytes determined from equilibrium competitio...

journal_title：FEBS letters

authors： Schwarz KR,Lanier SM,Carter EA,Graham RM,Homcy CJ

更新日期：1985-08-05 00:00:00

abstract：:IGF-I receptors were partially purified from red and white skeletal muscle by lectin-affinity chromatography and the resultant fraction was depleted of insulin receptors by insulin affinity chromatography. Equilibrium binding of 125I-IGF-I to receptor preparations from red and white muscle yielded identical Scatchard ...

journal_title：FEBS letters

authors： Zorzano A,James DE,Ruderman NB,Pilch PF

更新日期：1988-07-18 00:00:00

abstract：:We have studied the incorporation of [(14)C]serine and of [(3)H]sphingosine into sphingomyelin in the presence or absence of brefeldin A (BFA) in three different cell types. Administration of BFA (1 microgram/ml) to fibroblasts for 24 h increased the incorporation of label into sphingomyelin 1.5-3 fold compared with u...

journal_title：FEBS letters

authors： Sadeghlar F,Sandhoff K,van Echten-Deckert G

更新日期：2000-07-28 00:00:00

abstract：:A serum-free medium conditioned by U937, a human cell line of monocyte/macrophage origin, was found capable of inducing apoptosis on exponentially growing U937 cells themselves (autocrine suicide). The apoptosis-inducing agent is a macromolecule and possibly a protein (SKT factor), with a relative molecular mass in th...

journal_title：FEBS letters

authors： Ghibelli L,Coppola S,Nosseri C,Bergamini A,Beninati S

更新日期：1994-05-09 00:00:00

abstract：:Using the method of contextual analysis, we have studied the collection of somatic mutations in immunoglobulin genes. It has been found that the emergence of somatic mutations can be based on the reparation of complementarity violations in the heteroduplexes corresponding to complementary palindromes or direct repeats...

journal_title：FEBS letters

authors： Kolchanov NA,Solovyov VV,Rogozin IB

更新日期：1987-04-06 00:00:00

abstract：:Mona is an SH3 and SH2 domain-containing adapter molecule that is induced during monocytic differentiation. Here we have first shown that M-CSFR is the major Mona partner in M-CSF signaling, the interaction being mediated through tyrosine 697 of the receptor. Next we asked whether Mona expression would alter the Ras/M...

journal_title：FEBS letters

authors： Bourgin C,Bourette R,Mouchiroud G,Arnaud S

更新日期：2000-09-01 00:00:00

abstract：:Analyzing the primary structure we predicted that calponin may interact with phospholipids. In order to check this suggestion we investigated the interaction of calponin with phospholipids by ultracentrifugation, light scattering, vesicles leakage and differential scanning calorimetry. In agreement with our prediction...

journal_title：FEBS letters

authors： Bogatcheva NV,Gusev NB

更新日期：1995-09-04 00:00:00

abstract：:A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1-10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamine sulphate. When high concentrations of bFGF were added, a preferential effe...

journal_title：FEBS letters

authors： Schofield PN,Granerus M,Lee A,Ektröm TJ,Engström W

更新日期：1992-02-24 00:00:00