Induction of proline-rich tyrosine kinase2 (Pyk2) through C/EBPbeta is involved in PMA-induced monocyte differentiation.


:Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute promyelocytic leukemia cells, the expression of Pyk2 was increased in parallel with differentiation, and inhibited by PD98059, indicating Pyk2 expression is regulated through MAPK/ERK pathway. Dominant-negative kinase-deficient mutant of Pyk2 reduced the differentiation of NB4 cells in response to phorbol 12-mystate 13-acetate. Transcription factor CCAAT enhancer-binding protein (C/EBP) beta was required to induce Pyk2 expression in promoter analysis. These results suggest that Pyk2 is induced and involved in monocyte differentiation and C/EBPbeta is a critical regulator of the Pyk2 expression.




FEBS letters


Park MH,Park SY,Kim Y




Has Abstract


2008-02-06 00:00:00














  • Spectroscopic identification of the haem ligands of cellobiose oxidase.

    abstract::A spectroscopic study of the flavocytochrome b enzyme, cellobiose oxidase, employing optical, NMR, EPR and near infra-red MCD techniques, has identified the axial ligands of the b-type haem. These are a histidine and a methionine, and this ligation set is discussed in relation to the functional role of the haem group....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cox MC,Rogers MS,Cheesman M,Jones GD,Thomson AJ,Wilson MT,Moore GR

    更新日期:1992-07-28 00:00:00

  • MDR-like ABC transporter AtPGP4 is involved in auxin-mediated lateral root and root hair development.

    abstract::Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Santelia D,Vincenzetti V,Azzarello E,Bovet L,Fukao Y,Düchtig P,Mancuso S,Martinoia E,Geisler M

    更新日期:2005-10-10 00:00:00

  • Neprylisin decreases uniformly in Alzheimer's disease and in normal aging.

    abstract::The proteolysis of beta-amyloid (Abeta) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease in neprilysin levels contributes to the accumulation of amyloid deposits not only in AD but also in the normal aging. We analyzed neprilysin mRNA and pro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Russo R,Borghi R,Markesbery W,Tabaton M,Piccini A

    更新日期:2005-11-07 00:00:00

  • Novel bioactive lipodepsipeptides from Pseudomonas syringae: the pseudomycins.

    abstract::The covalent structure and most of the stereochemistry of the pseudomycins, bioactive metabolites of a transposon-generated mutant of a Pseudomonas syringae wild-type strain proposed for the biological control of Dutch elm disease, have been determined. While two pseudomycins are identical to the known syringopeptins ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ballio A,Bossa F,Di Giorgio D,Ferranti P,Paci M,Pucci P,Scaloni A,Segre A,Strobel GA

    更新日期:1994-11-21 00:00:00

  • Anaerobic carotenoid biosynthesis in Rhodobacter sphaeroides 2.4.1: H2O is a source of oxygen for the 1-methoxy group of spheroidene but not for the 2-oxo group of spheroidenone.

    abstract::Anaerobic biosynthesis of carotenoids in the purple facultative photosynthetic bacterium Rhodobacter sphaeroides was studied using mass spectrometry. We have demonstrated that (18)O from H2(18)O was incorporated into the 1-methoxy group of spheroidene and spheroidenone, the two major carotenoids produced by this bacte...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yeliseev AA,Kaplan S

    更新日期:1997-02-10 00:00:00

  • The G protein-coupled receptor rhodopsin in the native membrane.

    abstract::The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation of even larger oligomeric complexes. Here, we have investigated the organization of the prototypical GPCR rhodopsin in its native membrane by electron and atomic force microscopy (AFM). Disc membranes ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fotiadis D,Liang Y,Filipek S,Saperstein DA,Engel A,Palczewski K

    更新日期:2004-04-30 00:00:00

  • Inhibition by somatostatin of the vasopressin-stimulated adenylate cyclase in a kidney-derived line of cells grown in defined medium.

    abstract::LLC-PK1L cells, a kidney-derived cell line grown in defined medium, possess a vasopressin-sensitive adenylate cyclase. Somatostatin was able to inhibit the vasopressin-induced increase in adenylate cyclase activity, without affecting the basal enzyme activity. This inhibition was competitive. No effect of somatostatin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roy C

    更新日期:1984-04-24 00:00:00

  • Permeation and gating of alpha1 glycine-gated channels expressed at low and high density in Xenopus oocyte.

    abstract::When a high density of alpha1-subunit glycine receptor (GlyR) is expressed in Xenopus oocytes, two populations of channels can be distinguished according to their apparent affinity for glycine which differs 5- to 6-fold. To compare the open pore diameter of these channels, the relative permeability of formate with res...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Maammar M,Rodeau JL,Taleb O

    更新日期:1997-09-01 00:00:00

  • Differential activation of STATs 3 and 5 during mammary gland development.

    abstract::We have investigated the activity of STAT family members throughout a mammary developmental cycle. Transcripts for Stat 5 were upregulated during pregnancy whilst STAT1 and STAT3 mRNAs were expressed at constant levels. DNA binding complexes containing both STAT5a and 5b showed differing affinities for two naturally o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Philp JA,Burdon TG,Watson CJ

    更新日期:1996-10-28 00:00:00

  • Prorenin processing by cathepsin B in vitro and in transfected cells.

    abstract::Renin, which catalyzes the initial proteolytic cleavage reaction in the production of angiotensins, is first synthesized as a zymogen, prorenin, and requires the proteolytic removal of an amino-terminal prosegment for activation in vivo. The lysosomal hydrolase cathepsin B has been proposed as a prorenin processing en...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jutras I,Reudelhuber TL

    更新日期:1999-01-22 00:00:00

  • γ-Glutamyl kinase is involved in selective autophagy of ribosomes in Saccharomyces cerevisiae.

    abstract::γ-Glutamyl kinase (GK; the PRO1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild-type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO1 genetically interact...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Tatehashi Y,Watanabe D,Takagi H

    更新日期:2016-09-01 00:00:00

  • Protein syntheis in brain mitochondrial and synaptosomal preparations.

    abstract::The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Morgan IG

    更新日期:1970-10-16 00:00:00

  • A putative internal promoter in the 16 S/23 S intergenic spacer of the rRNA operon of archaebacteria and eubacteria.

    abstract::The existence of the internal promoter Pi in the 16 S/23 S intergenic spacers of the rRNA operons of an eubacterium Escherichia coli and archaebacterium Halobacterium halobium is proposed. The possible functional significance of these promoters is discussed. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mankin AS,Skripkin EA,Kagramanova VK

    更新日期:1987-07-27 00:00:00

  • Identification of different specificity requirements between SGK1 and PKBalpha.

    abstract::NDRG1 is phosphorylated by SGK1 (but not PKB) in vivo at three residues each contained within three nonapeptide repeats. Here, we demonstrate that this nonapeptide, like the NDRG1 protein, is phosphorylated by SGK1, but not by PKBalpha or RSK1 in vitro. The inability of PKBalpha and RSK1 to phosphorylate the nonapepti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murray JT,Cummings LA,Bloomberg GB,Cohen P

    更新日期:2005-02-14 00:00:00

  • Regulation of fructose 2,6-bisphosphate levels in cold-acclimated brown adipose tissue of rat.

    abstract::The effects of cold exposure and T4 administration on fructose 2,6-bisphosphate levels, phosphofructokinase-2 and pyruvate kinase activities were examined in rat brown adipose tissue. Cold adaptation (14 days) gave rise to a 2-fold increase in the amount of fructose 2,6-bisphosphate and phosphofructokinase-2 activity,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sobrino F,Gualberto A,Pintado E

    更新日期:1988-02-29 00:00:00

  • Lipopolysaccharide-binding protein mediates CD14-independent intercalation of lipopolysaccharide into phospholipid membranes.

    abstract::Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects. Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent. Here, we demonstrate by resonance energy transfer (RET) techniq...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schromm AB,Brandenburg K,Rietschel ET,Flad HD,Carroll SF,Seydel U

    更新日期:1996-12-16 00:00:00

  • Cloning and sequence analysis of a cDNA encoding rice glutaredoxin.

    abstract::A full-length cDNA clone (RASC8) encoding glutaredoxin (thioltransferase) was isolated from a cDNA library of an aleurone layer prepared from a developing seed of rice (Oryza sativa L.). RASC8, 568bp in length, contained an ATG codon and two possible polyadenylation signals, and encoded 112 amino acid residues. Cys-Pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Minakuchi K,Yabushita T,Masumura T,Ichihara K,Tanaka K

    更新日期:1994-01-10 00:00:00

  • Calmodulin interacts with the cytoplasmic tails of the parathyroid hormone 1 receptor and a sub-set of class b G-protein coupled receptors.

    abstract::Parathyroid hormone (PTH) binds to its receptor (PTH 1 receptor, PTH1R) and activates multiple pathways. The PTH1R, a class b GPCR, contains consensus calmodulin-binding motifs. The PTH1R cytoplasmic tail interacts with calmodulin in a calcium-dependent manner via the basic 1-5-8-14 motif. Calcium-dependent calmodulin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mahon MJ,Shimada M

    更新日期:2005-01-31 00:00:00

  • Gene relaxation and aging: changes in the abundance of rat ventral prostate SGP-2 (clusterin) and ornithine decarboxylase mRNAs.

    abstract::Sulfated glycoprotein 2 (SGP-2) mRNA progressively increased in the ventral prostate of the aging rat, reaching, at 24 months, 4-fold higher than at 3 months. Ornithine decarboxylase (ODC) mRNA peaked at 6 months (4-fold increase), and at 12 and 24 months was maintained at higher levels than at 3 months. ODC enzymatic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bettuzzi S,Strocchi P,Marinelli M,Astancolle S,Davalli P,Corti A

    更新日期:1994-07-18 00:00:00

  • DNMT1 cooperates with MBD4 to inhibit the expression of Glucocorticoid-induced TNFR-related protein in human T cells.

    abstract::Glucocorticoid-induced TNFR-related protein (GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a ChIP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 (MBD4) bind to the...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Wang S,Li Y,Zhu F,Lin F,Luo X,Zhao B,Zhang P,Li D,Gao Y,Liang R,Liu L,Tsun A,Yuan X,Wu K,Li B

    更新日期:2017-07-01 00:00:00

  • ATP synthesis catalyzed by the mitochondrial F1-F0 ATP synthase is not a reversal of its ATPase activity.

    abstract::The ADP(Mg2+)-deactivated oligomycin-sensitive F1-F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Syroeshkin AV,Vasilyeva EA,Vinogradov AD

    更新日期:1995-06-05 00:00:00

  • Most of the structural elements of the globular domain of murine prion protein form fibrils with predominant beta-sheet structure.

    abstract::The conversion of the cellular prion protein into the beta-sheet-rich scrapie prion protein is thought to be the key step in the pathogenesis of prion diseases. To gain insight into this structural conversion, we analyzed the intrinsic structural propensity of the amino acid sequence of the murine prion C-terminal dom...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jamin N,Coïc YM,Landon C,Ovtracht L,Baleux F,Neumann JM,Sanson A

    更新日期:2002-10-09 00:00:00

  • A large conductance ion channel in the nuclear envelope of a higher plant cell.

    abstract::To detect and characterize ion channel activity in the nuclear envelope of a higher plant cell, we performed patch clamp experiments on nuclei isolated from coconut endosperm cells and on giant liposomes containing nuclear envelope fragments prepared from the same cells. An ion channel exhibiting a number of conductan...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matzke AJ,Behensky C,Weiger T,Matzke MA

    更新日期:1992-05-04 00:00:00

  • Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity.

    abstract::We explored the interactive effects of endothelial nitric oxide synthase (eNOS) genotypes and cigarette smoking on protein levels and enzyme activity in 33 postpartum placentas. Whilst the eNOS protein levels were lower in the rare allele (0.48+/-0.11, n=9 vs. 1. 05+/-0.10, n=24, P<0.01), the eNOS enzyme activity was ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang XL,Sim AS,Wang MX,Murrell GA,Trudinger B,Wang J

    更新日期:2000-04-07 00:00:00

  • Two identical hydrophobic clusters are present on the same actin monomer: interaction between one myosin subfragment-1 and two actin monomers.

    abstract::Two-dimensional hydrophobic clusters analysis (HCA) was used to compare the distribution of hydrophobic clusters along various actin sequence. HCA-deduced patterns were not altered by amino-acid variations throughout the evolution of actin and we observed similar hydrophobic motifs comprising myosin subfragment-1 ATP-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Labbé JP,Boyer M,Benyamin Y

    更新日期:1995-10-16 00:00:00

  • Mechanism of transport of L-alanine by luminal-membrane vesicles from pars recta of rabbit proximal tubule.

    abstract::The characteristics of renal transport of L-alanine by luminal-membrane vesicles from proximal straight tubules (pars recta) of rabbit kidney were investigated. The following picture emerges from transport studies. Two electrogenic and Na+ requiring systems confined to this region of the nephron exist for the transpor...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vorum H,Jessen H,Jørgensen KE,Sheikh MI

    更新日期:1988-01-18 00:00:00

  • Diffusion of fluorescein-labelled molecules in suspensions of erythrocyte ghosts.

    abstract::The diffusion of fluorescein isothiocyanate-labelled dextran molecules in suspensions of centrifugally, tightly packed, erythrocyte ghosts was measured by fluorescence recovery after photobleaching. In comparison with diffusion in aqueous solution, the diffusion coefficients for probe molecules of varying size were ab...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Järnefelt J,Laurent T,Rigler R

    更新日期:1988-12-19 00:00:00

  • Activation of phospholipase D by osmotic cell swelling.

    abstract::In response to osmotic cell swelling, Intestine 407 cells react with a rapid and transient activation of phospholipase D (PLD). To investigate the role of PLD during the regulatory volume decrease, cells were treated with 1-butanol resulting in a depletion of PLD substrates. Activation of volume-regulated anion channe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tomassen SF,van der Wijk T,de Jonge HR,Tilly BC

    更新日期:2004-05-21 00:00:00

  • Fluorescence ratiometric detection of ligand-induced receptor internalization using extracellular coiled-coil tag-probe labeling.

    abstract::We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic rece...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yano Y,Matsuzaki K

    更新日期:2011-07-21 00:00:00

  • Bisphosphorylation of cardiac troponin I modulates the Ca(2+)-dependent binding of myosin subfragment S1 to reconstituted thin filaments.

    abstract::We have reconstituted thin filaments comprising pyrene-labelled actin (pyr-actin), tropomyosin (Tm) and cardiac troponin (cTn). cTn was isolated in two defined phosphorylation states; completely dephosphorylated on all subunits and with only the cTnI subunit bisphosphorylated. The thin filament was saturated with cTn ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Reiffert SU,Jaquet K,Heilmeyer LM Jr,Ritchie MD,Geeves MA

    更新日期:1996-04-08 00:00:00