GFPs of insertion mutation generated by molecular size-altering block shuffling.

abstract：:In Alzheimer disease (AD) the microtubule associated protein (MAP) tau is hyperphosphorylated at several sites. In the present study, like AD tau, tau in the human neuroblastoma SH-SY5Y was found to be hyperphosphorylated, at Ser-199/202, Thr-231, Ser-396 and Ser-404. However, in contrast to AD, the tau in SY5Y cells ...

journal_title：FEBS letters

authors： Tanaka T,Iqbal K,Trenkner E,Liu DJ,Grundke-Iqbal I

更新日期：1995-02-20 00:00:00

abstract：:To investigate the importance of the seventh residue of the second and third repeat fragments (R2 and R3 peptides) of the microtubule-binding domain (MBD) for tau filamentous assembly, the residues Lys and Pro were substituted (R2-K7P and R3-P7K). The filament formations of the R2 and R3 peptides were almost lost due ...

journal_title：FEBS letters

authors： Tokimasa M,Minoura K,Hiraoka S,Tomoo K,Sumida M,Taniguchi T,Ishida T

更新日期：2005-07-04 00:00:00

abstract：:Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR). Here, we show that this mode of regulation is exerted in a cell type-specific manner. Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation o...

journal_title：FEBS letters

authors： Hornstein E,Harel H,Levy G,Meyuhas O

更新日期：1999-08-27 00:00:00

abstract：:The 14 kDa protein was purified from human platelets. It displays high cytotoxic activity to the human ACL cells at 10 M concentration (21.8+/-7.1%). Its N-terminal sequence is YAPQXQFGP-, being highly homologous to region 241-249 residues of the human Cls complement component. ...

journal_title：FEBS letters

authors： Golubeva TS,Paromov VM,Tuguz AR,Chertov OY,Kiselevsky MV

更新日期：1997-04-01 00:00:00

abstract：:The present study investigated the mechanism by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) inhibit platelet activation induced by thromboxane A2. DHA was found to be more potent than EPA in blocking platelet aggregation induced by the stable thromboxane A2 mimetic, U46619. Furthermore, this inhib...

journal_title：FEBS letters

authors： Swann PG,Venton DL,Le Breton GC

更新日期：1989-01-30 00:00:00

abstract：:A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...

journal_title：FEBS letters

authors： Nonaka Y,Matsukawa N,Morohashi,Omura T,Ogihara T,Teraoka H,Okamoto M

更新日期：1989-09-11 00:00:00

abstract：:The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...

journal_title：FEBS letters

authors： Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu S

更新日期：2007-04-03 00:00:00

abstract：:Rats bearing the Yoshida AH-130 ascites hepatoma showed an increased expression of both uncoupling protein-2 (UCP2) (194%) and UCP3 (189%) mRNA levels in skeletal muscle 7 days after tumour inoculation. Interestingly, an even greater increase was observed in mRNA for both UCP2 (278%) and UCP3 (797%) in the pair-fed an...

journal_title：FEBS letters

authors： Sanchís D,Busquets S,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

更新日期：1998-10-09 00:00:00

abstract：:We investigated the signaling pathways participating in hyperosmotic regulation of the human taurine transporter (TAUT) by using specific inhibitors of various intracellular signaling molecules. Among them, the specific inhibitor of calcium/calmodulin-dependent protein kinase II (Ca(2+)/CaM kinase II) completely repre...

journal_title：FEBS letters

authors： Satsu H,Manabe M,Shimizu M

更新日期：2004-07-02 00:00:00

abstract：:In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...

journal_title：FEBS letters

authors： Martiny L,Dib K,Haye B,Corrèze C,Jacquemin C,Lambert B

更新日期：1991-07-29 00:00:00

abstract：:Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold,...

journal_title：FEBS letters

authors： McEachern F,Fisher LM

更新日期：1989-08-14 00:00:00

abstract：:The inositol (1,4,5)-trisphosphate receptor (InsP3R) is an intracellular calcium release channel that plays a crucial role in cell signaling. In Drosophila melanogaster, a single InsP3R gene (itpr) encodes a protein (DmInsP3R) that is approximately 60% conserved with mammalian InsP3Rs. The functional properties of wil...

journal_title：FEBS letters

authors： Srikanth S,Wang Z,Hasan G,Bezprozvanny I

更新日期：2004-09-24 00:00:00

abstract：:The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...

journal_title：FEBS letters

authors： Brzezinski P,Thörnström PE,Malmström BG

更新日期：1986-01-01 00:00:00

abstract：:Mox1 and Mox2 homeobox genes have been shown to be critical in axial skeleton and in limb muscle development respectively. Pax1 and Pax3 gene products are also implicated in these processes. Mox and Pax expression patterns are highly overlapping both spatially and temporally during embryonic development. We show here ...

journal_title：FEBS letters

authors： Stamataki D,Kastrinaki M,Mankoo BS,Pachnis V,Karagogeos D

更新日期：2001-06-22 00:00:00

abstract：:The amino acid sequences of the polypeptide chains of the acetylcholine receptor have recently been published. From the hydrophilicity profiles, it has been proposed that residues 161-166 of the alpha-chain might be an important antigenic site. We have synthesised a peptide containing this sequence and raised antisera...

journal_title：FEBS letters

authors： Juillerat MA,Barkas T,Tzartos SJ

更新日期：1984-03-12 00:00:00

abstract：:The rat synapse associated protein SAP90 is a member of a superfamily of potential guanylate kinases localized at cell-cell contact sites. This superfamily includes the synapse associated protein SAP97, a close relative of SAP90, the Drosophila tumor suppressor gene product dlg-Ap, the mammalian zonula occludens prote...

journal_title：FEBS letters

authors： Kistner U,Garner CC,Linial M

更新日期：1995-02-13 00:00:00

abstract：:The function of the domains of Trichoderma reesei endoglucanase I (EGI) has been studied. Truncated EGI proteins were expressed from the 3'-end deleted cDNAs in the yeast Saccharomyces cerevisiae under the control of the ADC1 expression cassette. EGI protein was detected by monoclonal antibody EI-2 and EGI activity as...

journal_title：FEBS letters

authors： Aho S

更新日期：1991-10-07 00:00:00

abstract：:The isocitrate lyase-encoding gene AgICL1 from the filamentous hemiascomycete Ashbya gossypii was isolated by heterologous complementation of a Saccharomyces cerevisiae icl1d mutant. The open reading frame of 1680 bp encoded a protein of 560 amino acids with a calculated molecular weight of 62584. Disruption of the Ag...

journal_title：FEBS letters

authors： Maeting I,Schmidt G,Sahm H,Revuelta JL,Stierhof YD,Stahmann KP

更新日期：1999-02-05 00:00:00

abstract：:Using antibody against the Rho binding domain of ROKalpha, two neuronal phosphoproteins of 62 and 80 kDa were co-immunoprecipitated from brain extracts. Peptide analysis revealed their identity as collapsin response mediator proteins (CRMPs); p62 was CRMP-2 whereas p80 was a novel splice form of CRMP-1 with an extende...

journal_title：FEBS letters

authors： Leung T,Ng Y,Cheong A,Ng CH,Tan I,Hall C,Lim L

更新日期：2002-12-18 00:00:00

abstract：:Oligonucleotides 5'-d(CT)5-L-d(AG)5-L-d(GA)5-3' and 5'-d(GA)5-L-d(TC)5-L-d(GA)5-3' [L = pO(CH2CH2O)3p] were studied by thermal denaturation, chemical modification and binding of fluorescent dyes. Both oligonucleotides are shown to fold back on itself twice forming at pH 7 a sufficiently stable triplex ether with antip...

journal_title：FEBS letters

authors： Shchyolkina AK,Borisova OF,Minyat EE,Timofeev EN,Il'icheva IA,Khomyakova EB,Florentiev VL

更新日期：1995-06-19 00:00:00

abstract：:Haloalkane dehalogenase hydrolyses various 1-halo-n-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed to an amide by sit...

journal_title：FEBS letters

authors： Pries F,Kingma J,Janssen DB

更新日期：1995-01-23 00:00:00

abstract：:Prolactin (PRL) has been implicated as a modulator of immune function, and some of its actions may be linked to NO synthesis. Because NO acts as a mediator of inflammation, we speculated that an inflammatory milieu could unmask pathways by which PRL could affect NO synthesis. Here, we show that pro-inflammatory cytoki...

journal_title：FEBS letters

authors： Corbacho AM,Macotela Y,Nava G,Eiserich JP,Cross CE,Martínez de la Escalera G,Clapp C

更新日期：2003-06-05 00:00:00

abstract：:Contribution of sphingosine kinase (SPK)-catalyzed production of sphingosine-1-phosphate (SPP), in comparison to phospholipase C (PLC), to Ca(2+) signalling by epidermal growth factor (EGF) was studied in two HEK-293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca(2...

journal_title：FEBS letters

authors： Meyer zu Heringdorf D,Lass H,Kuchar I,Alemany R,Guo Y,Schmidt M,Jakobs KH

更新日期：1999-11-19 00:00:00

abstract：:The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...

journal_title：FEBS letters

authors： Cai Q,Wang W,Gao Y,Yang Y,Zhu Z,Fan Q

更新日期：2009-10-06 00:00:00

abstract：:Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposur...

journal_title：FEBS letters

authors： Chen Y,Cai J,Jones DP

更新日期：2006-12-11 00:00:00

abstract：:The present one-drug-one-target paradigm in drug discovery has been considered partially responsible for the more-funding-less-drug predicament in modern pharmaceutical industry. To hit the multiple targets implicated in complex diseases, two strategies, based on multicomponent or single-ingredient, are conceivable. A...

journal_title：FEBS letters

authors： Zhang HY

更新日期：2005-10-10 00:00:00

abstract：:A technique has been developed to probe directly RecA-DNA interactions by the use of the fluorescent chromophore, (+)anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), covalently attached to DNA. The 24-mer oligonucleotide 5'-d(CTACTAAACATGTACAAATCATCC) was specifically modified on the exocyclic nitrogen of the ...

journal_title：FEBS letters

authors： Wittung P,Funk M,Jernström B,Nordén B,Takahashi M

更新日期：1995-07-10 00:00:00

abstract：:It has previously been shown that the levels of poly(ADP-ribose)polymerase and polymers of ADP-ribose that co-purify with the nuclear matrix in regenerating liver fluctuate with the levels of in vivo DNA replication [(1988) FEBS Lett. 236, 362-366]. We have now electrophoretically identified lamins A and C, and poly(A...

journal_title：FEBS letters

authors： Pedraza-Reyes M,Alvarez-Gonzalez R

更新日期：1990-12-17 00:00:00

abstract：:The glycogen-binding (G) subunit of protein phosphatase-1 is phosphorylated in vivo. In rabbits injected with propranolol the serine residue termed site-1 was phosphorylated in 56% of the molecules isolated, and phosphorylation increased to 82% after administration of adrenalin. It is concluded that the G-subunit is a...

journal_title：FEBS letters

authors： MacKintosh C,Campbell DG,Hiraga A,Cohen P

更新日期：1988-07-04 00:00:00

abstract：:Oligonucleotide probes based on the known amino acid sequence of salmon calcitonin were used to screen a cDNA library obtained from ultimobranchial glands of salmon for clones encoding salmon calcitonin. From the cDNA sequence of strongly hybridizing clones the complete primary structure of the calcitonin precursor co...

journal_title：FEBS letters

authors： Pöschl E,Lindley I,Hofer E,Seifert JM,Brunowsky W,Besemer J

更新日期：1987-12-21 00:00:00