Structural and functional analysis of Trichoderma reesei endoglucanase I expressed in yeast Saccharomyces cerevisiae.

abstract：:An interpretation of alternative crystal structures of the erythropoietin receptor, with and without ligand, led to the proposal of a scissor-like mechanism of activation. This model has been propagated in the literature and is still being used to interpret crystal structures of related type-I cytokine receptors. Here...

journal_title：FEBS letters

authors： Corbett MS,Poger D,Mark AE

更新日期：2016-09-01 00:00:00

abstract：:The main immunogenic region (MIR) of the acetylcholine receptor (AChR) is the target for the majority of high-affinity autoantibodies produced in myasthenia gravis patients. Some monoclonal antibodies (mAbs) to the MIR bind specifically, but with low affinity, to synthetic AChR alpha subunit peptides with the sequence...

journal_title：FEBS letters

authors： Saedi MS,Anand R,Conroy WG,Lindstrom J

更新日期：1990-07-02 00:00:00

abstract：:A cell culture model of blood-brain barrier (BBB, coculture of rat brain endothelial cells with rat astrocytes) was used to investigate the effect of nitric oxide (.NO) on the damage of the BBB induced by hypoxia/reoxygenation (H/R). Permeability coefficient of fluorescein across the endothelium was used as a marker o...

journal_title：FEBS letters

authors： Utepbergenov DI,Mertsch K,Sporbert A,Tenz K,Paul M,Haseloff RF,Blasig IE

更新日期：1998-03-13 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP c...

journal_title：FEBS letters

authors： Jarmuszkiewicz W,Antos N,Swida A,Czarna M,Sluse FE

更新日期：2004-07-02 00:00:00

abstract：:The sequences of rat testis carbonyl reductase (rCR1) and rat ovary carbonyl reductase (rCR2) are 98% identical, differing only at amino acids 140, 141, 143, 235 and 238. Despite such strong sequence identity, we find that rCR1 and rCR2 have different catalytic constants for metabolism of menadione and 4-benzoyl-pyrid...

journal_title：FEBS letters

authors： Sciotti MA,Tam S,Wermuth B,Baker ME

更新日期：2006-01-09 00:00:00

abstract：:DNA complementary to chicken ultimobranchial gland mRNA was cloned into the Pst I site of plasmid vector pBR322. A plasmid was selected by DNA-mRNA hybridization. We report here the partial nucleotide sequence of chicken calcitonin mRNA and the deduced complete amino acid sequence of chicken calcitonin. ...

journal_title：FEBS letters

authors： Lasmoles F,Jullienne A,Desplan C,Milhaud G,Moukhtar MS

更新日期：1985-01-21 00:00:00

abstract：:Coagulation factor VII contains ten gamma-carboxyglutamic acid residues in the N-terminal region (Gla-domain) which are essential for the hemostatic function of FVII. The present study shows that granulocyte cathepsin G degrades the Gla-domain of FVIIa in vitro. Characterization of the truncated FVIIa by SDS-PAGE and ...

journal_title：FEBS letters

authors： Nicolaisen EM,Petersen LC,Thim L,Jacobsen JK,Christensen M,Hedner U

更新日期：1992-07-20 00:00:00

abstract：:Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the s...

journal_title：FEBS letters

authors： Meek DW,Nimmo HG

更新日期：1983-08-22 00:00:00

abstract：:Amiloride, a specific inhibitor for the Na(+)-driven flagellar motors of alkalophilic Bacillus, is known to inhibit secondarily the growth of alkalophiles. The motility of a marine Vibrio, V. alginolyticus, was almost completely inhibited by 2 mM amiloride either at pH 7.0 or 8.5. We found that this concentration of a...

journal_title：FEBS letters

authors： Atsumi T,Maekawa Y,Tokuda H,Imae Y

更新日期：1992-12-14 00:00:00

abstract：:Lipid droplets (LDs) were perceived as static storage deposits, which passively participate in the energy homeostasis of both cells and entire organisms. However, this view has changed recently after the realization of a complex and highly dynamic LD proteome. The proteome contains key components of the fat mobilizati...

journal_title：FEBS letters

authors： Beller M,Thiel K,Thul PJ,Jäckle H

更新日期：2010-06-03 00:00:00

abstract：:We previously showed that the post-translational cleavage of alphav subunit is essential for integrin-dependent signalling and cell adhesion. Here, we report that blocking alphav subunit cleavage by expression of alpha1-PDX, a convertase inhibitor, modified the capacity of cells to change shape, via a remodelling of t...

journal_title：FEBS letters

authors： Berthet V,Rigot V,Nejjari M,Marvaldi J,Luis J

更新日期：2004-01-16 00:00:00

abstract：:Persistent tumour necrosis factor alpha (TNF-alpha) exposure uncouples proximal T-cell receptor (TCR)-signalling events. Here, we demonstrate that chronic TNF-alpha exposure also attenuates signalling distal to the TCR, by specifically inhibiting Ca2+ influx evoked by thapsigargin in CD4+ T-cells. Mitogen-induced Ca2+...

journal_title：FEBS letters

authors： Church LD,Goodall JE,Rider DA,Bacon PA,Young SP

更新日期：2005-02-28 00:00:00

abstract：:We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to...

journal_title：FEBS letters

authors： Ito T,Kito K,Adati N,Mitsui Y,Hagiwara H,Sakaki Y

更新日期：1994-09-05 00:00:00

abstract：:The fluorescence intensity of the Ca2+,Mg2(+)-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 microM calcium. The observed enhancement indicates that the fluorophore, ...

journal_title：FEBS letters

authors： Bailin G,Huang JR

更新日期：1990-01-01 00:00:00

abstract：:Criteria for the design of peptide vaccines to prevent AIDS are presented. The best vaccine candidates contain both B and T lymphocyte-defined epitopes in regions conserved in sequence between viral isolates. We propose that attention should focus on proteins specified by the gag and, possibly, pol genes in addition t...

journal_title：FEBS letters

authors： Sternberg MJ,Barton GJ,Zvelebil MJ,Cookson J,Coates AR

更新日期：1987-06-29 00:00:00

abstract：:It has long been known from fluorescence recovery after photobleaching experiments that the mobility of most cell surface receptors is much smaller than expected for free diffusion of proteins in a fluid lipid bilayer. Single-particle tracking experiments are currently revealing the complexity of the constraints to fr...

journal_title：FEBS letters

authors： Cherry RJ,Smith PR,Morrison IE,Fernandez N

更新日期：1998-06-23 00:00:00

abstract：:Transthyretin (TTR) is a circulatory protein which plays an important role in the transport of both thyroid hormone and retinol. Hep G2 cells, a human hepatoma-derived cell line, have been used extensively in studies of protein secretion by liver cells. The original description of this cell line indicated that this li...

journal_title：FEBS letters

authors： Blaner WS,Bonifácio MJ,Feldman HD,Piantedosi R,Saraiva MJ

更新日期：1991-08-05 00:00:00

abstract：:Epidermal growth factor (EGF) dose-dependently enhanced the induction of tyrosine aminotransferase and tryptophan oxygenase by glucocorticoids in primary cultures of adult rat hepatocytes without itself having any effect on these enzymes in the absence of glucocorticoids. The amplifications were observed even with dex...

journal_title：FEBS letters

authors： Kido H,Fukusen N,Katunuma N

更新日期：1987-11-02 00:00:00

abstract：:Early results showed the emanation of chromatin fibers from mitotic chromosomes and nuclei swollen with water. In contrast, under metaphase ionic conditions, it was found that chromatin from mitotic chromosomes is planar and forms multilayered plates. Here, we show that in buffers containing interphase cation concentr...

journal_title：FEBS letters

authors： Chicano A,Daban JR

更新日期：2019-04-01 00:00:00

abstract：:Toll-like receptors (TLRs) are crucial components of the innate immune system, coupling pathogen recognition to a cellular response. We used the MAPPIT mammalian two-hybrid technique to investigate protein-protein interactions in the early steps in TLR signalling. A partial TLR-adaptor interaction map was constructed ...

journal_title：FEBS letters

authors： Ulrichts P,Peelman F,Beyaert R,Tavernier J

更新日期：2007-02-20 00:00:00

abstract：:Three homologous aspartate aminotransferases with virtually identical spatial structures and pairwise amino acid sequence identities of > 40% differ markedly with respect to the yield of renaturation upon dilution from 6 M guanidine hydrochloride (mitochondrial < cytosolic < Escherichia coli). The enzymes also respond...

journal_title：FEBS letters

authors： Widmann M,Christen P

更新日期：1995-12-27 00:00:00

abstract：:The mongoose AChR alpha-subunit has been cloned and shown to be highly homologous to other AChR alpha-subunits, with only six differences in amino acid residues at positions that are conserved in animal species that bind alpha-bungarotoxin (alpha-BTX). Four of these six substitutions cluster in the ligand binding site...

journal_title：FEBS letters

authors： Asher O,Jensen BS,Lupu-Meiri M,Oron Y,Fuchs S

更新日期：1998-04-17 00:00:00

abstract：:The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutans, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequence...

journal_title：FEBS letters

authors： Kelly C,Evans P,Bergmeier L,Lee SF,Progulske-Fox A,Harris AC,Aitken A,Bleiweis AS,Lehner T

更新日期：1989-11-20 00:00:00

abstract：:Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...

journal_title：FEBS letters

authors： Houston P,Goodman J,Lewis A,Campbell CJ,Braddock M

更新日期：2001-03-09 00:00:00

abstract：:The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to d...

journal_title：FEBS letters

authors： Churchich JE,Kim DS,Schnackerz KD

更新日期：1983-08-22 00:00:00

abstract：:The red fluorescent protein, DsRed, recently cloned from coral Discosoma sp. has one of the longest fluorescence waves and one of the most complex absorbance spectra among the family of fluorescent proteins. In this work we found that with time DsRed fluorescence decreases under mildly acidic conditions (pH 4.0-4.8) i...

journal_title：FEBS letters

authors： Vrzheshch PV,Akovbian NA,Varfolomeyev SD,Verkhusha VV

更新日期：2000-12-29 00:00:00

abstract：:A stable complex of the chaperonins, cpn60 and cpn10 (Escherichia coli GroEL and GroES homologues), from the extremely thermophilic bacterium Thermus thermophilus has been isolated and crystallized. The crystals have dimensions up to 30 x 200 x 200 microns. Ultra-thin sections of the crystals estimated by electron mic...

journal_title：FEBS letters

authors： Lissin NM,Sedelnikova SE,Ryazantsev SN

更新日期：1992-10-12 00:00:00

abstract：:Various molecular mechanisms are involved in the efficacy of arsenic trioxide (ATO) against malignant hematologic and some solid tumors. FLICE-like inhibitory protein (FLIP) is an inhibitor of apoptosis mediated by death receptors. In this study, we identified a new link between the down-regulation of cellular FLIP(L)...

journal_title：FEBS letters

authors： Zhang G,Liu J,Zhang Y,Qu J,Xu L,Zheng H,Liu Y,Qu X

更新日期：2012-09-21 00:00:00

abstract：:We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scat...

journal_title：FEBS letters

authors： He C,Gu Q,Huang M,Xing J,Chen J

更新日期：2013-01-31 00:00:00

abstract：:Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with re...

journal_title：FEBS letters

authors： Misra HS,Khairnar NP,Barik A,Indira Priyadarsini K,Mohan H,Apte SK

更新日期：2004-12-03 00:00:00