Abstract:
:We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such 'Fluorescent Differential Display (FDD)' techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ito T,Kito K,Adati N,Mitsui Y,Hagiwara H,Sakaki Ydoi
10.1016/0014-5793(94)00867-1subject
Has Abstractpub_date
1994-09-05 00:00:00pages
231-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(94)00867-1journal_volume
351pub_type
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