Abstract:
:Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here we show that erythrocytes of Panx1(-/-) mice lend further support to this conclusion. However, ATP release, although attenuated, was still observed in Panx1(-/-) mouse erythrocytes. In contrast to Panx1(+/+) cells, this release was not correlated with uptake of extracellularly applied dyes, was insensitive to Panx1 channel blockers, and was inhibited by dipyridamole and stimulated by iloprost. Thus, in erythrocytes, two independent pathways mediate the release of ATP. We also show that glyburide is a strong inhibitor of Panx1 channels.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Qiu F,Wang J,Spray DC,Scemes E,Dahl Gdoi
10.1016/j.febslet.2011.09.033subject
Has Abstractpub_date
2011-11-04 00:00:00pages
3430-5issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00712-5journal_volume
585pub_type
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