5'-Nucleotidase is activated upon cholesterol-depletion of liver plasma membranes.

abstract：:The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% ...

journal_title：FEBS letters

authors： Guo W,Roth D,Gatti E,De Camilli P,Novick P

更新日期：1997-03-10 00:00:00

abstract：:We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or f...

journal_title：FEBS letters

authors： Romero LC,Biswal B,Song PS

更新日期：1991-05-06 00:00:00

abstract：:Telomerase is a multimolecular complex of reverse transcriptase, RNA template, and regulatory proteins. It has two known functions: catalysis of the addition of [TTAGGG] repeats to telomeric DNA and the activation of various genes controlling cell proliferation. The possible coordination of these two functions is a ke...

journal_title：FEBS letters

authors： Wolfrom C,Martin OC,Laurent M,Deschatrette J

更新日期：2007-01-09 00:00:00

abstract：:In the rat liver, transcription of the serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) gene occurs from two sites, +1 and +66, in exon 1, resulting in the formation of two mRNAs, one for a precursor of mitochondrial SPT/AGT and the other for peroxisomal SPT/AGT, respectively. In this study, we attempted ...

journal_title：FEBS letters

authors： Sugiyama T,Uchida C,Oda T,Kitagawa M,Hayashi H,Ichiyama A

更新日期：2001-11-09 00:00:00

abstract：:Calpain, a Ca2+-dependent biomodulator, alters the properties of substrate proteins by cleaving them at a limited number of specific sites. Recent studies of the structure-function relationship of calpain and X-ray analysis of its Ca2+-binding domain have revealed hitherto unknown features of the regulation of calpain...

journal_title：FEBS letters

authors： Suzuki K,Sorimachi H

更新日期：1998-08-14 00:00:00

abstract：:The ATPase Cdc48 is required for membrane fusion and protein degradation. Recently it has been suggested that Cdc48 in a complex with Ufd1 and Npl4 acts as an ubiquitin-dependent chaperone. Here it is shown that recombinant Cdc48 alone can distinguish between the native and the non-native conformation of model substra...

journal_title：FEBS letters

authors： Thoms S

更新日期：2002-06-05 00:00:00

abstract：:The autocrine motility factor (AMF) is a multifunctional protein that is involved in tumor progression including enhanced invasiveness via induction of matrix metalloproteinase-3 (MMP3). The increase in MMP3 was found in an AMF-high production tumor cell line, and c-Jun, c-Fos and mitogen-activated protein kinases (MA...

journal_title：FEBS letters

authors： Haga A,Funasaka T,Deyashiki Y,Raz A

更新日期：2008-06-11 00:00:00

abstract：:The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid ca...

journal_title：FEBS letters

authors： Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón T

更新日期：1986-08-11 00:00:00

abstract：:In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipit...

journal_title：FEBS letters

authors： Mattei V,Garofalo T,Misasi R,Circella A,Manganelli V,Lucania G,Pavan A,Sorice M

更新日期：2004-02-27 00:00:00

abstract：:Familial porphyria cutanea tarda (PCT) is a photocutaneous disease in which subnormal activity of uroporphyrinogen decarboxylase is observed both in the liver and red cells. Hepatic iron plays a key role in the genesis of overt biochemical and clinical PCT. In this report, we have studied the properties of 10 000-fold...

journal_title：FEBS letters

authors： Mukerji SK,Pimstone NR,Tan KT

更新日期：1985-09-23 00:00:00

abstract：:The band 3 protein-mediated, valinomycin-induced KCl efflux continues to increase with increasing [KCl] when the Cl-/Cl- equilibrium exchange becomes saturated. This suggests the existence of a band 3-mediated component of Cl- flux that contributes to the electrical conductance without being related to slippage; i.e.,...

journal_title：FEBS letters

authors： Kaplan JH,Pring M,Passow H

更新日期：1983-05-30 00:00:00

abstract：:Myotonic dystrophy protein kinase (DMPK) and Rho-kinase are related. An important function of Rho-kinase is to phosphorylate the myosin-binding subunit of myosin phosphatase (MYPT1) and inhibit phosphatase activity. Experiments were carried out to determine if DMPK could function similarly. MYPT1 was phosphorylated by...

journal_title：FEBS letters

authors： Murányi A,Zhang R,Liu F,Hirano K,Ito M,Epstein HF,Hartshorne DJ

更新日期：2001-03-30 00:00:00

abstract：:The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...

journal_title：FEBS letters

authors： Miyamoto K,Nishimura K,Masuda T,Tsuji H,Inokuchi H

更新日期：1992-10-05 00:00:00

abstract：:WT1 was isolated as a tumor suppressor gene of Wilms tumor. However, high expression of WT1 correlates with poor prognosis in acute leukemia. In addition suppression of WT1 expression by WT1 anti-sense oligonucleotide inhibits proliferation of leukemia cells, suggesting that WT1 is important for their proliferation. T...

journal_title：FEBS letters

authors： Murata Y,Kudoh T,Sugiyama H,Toyoshima K,Akiyama T

更新日期：1997-06-02 00:00:00

abstract：:KOR-3, also known as ORL-1, is a member of the opioid receptor family, encoding the murine receptor for orphanin FQ/nociceptin. In the current studies we have identified five different splice variants of KOR-3 in mouse brain, three of which have not been previously reported. In addition to variants with a 15 bp deleti...

journal_title：FEBS letters

authors： Pan YX,Xu J,Wan BL,Zuckerman A,Pasternak GW

更新日期：1998-09-11 00:00:00

abstract：:Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...

journal_title：FEBS letters

authors： Adams B,Fowler R,Hudson M,Pain RH

更新日期：1996-04-29 00:00:00

abstract：:Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope...

journal_title：FEBS letters

authors： Guihard G,Proteau S,Payet MD,Escande D,Rousseau E

更新日期：2000-07-07 00:00:00

abstract：:Parasitic helminths remain major pathogens of both humans and animals throughout the world. The success of helminth infections depends on the capacity of the parasite to counteract host immune responses but also to exploit host-derived signal molecules for its development. Recent progress has been made in the characte...

journal_title：FEBS letters

authors： Dissous C,Khayath N,Vicogne J,Capron M

更新日期：2006-05-22 00:00:00

abstract：:Astrocytes (AC) induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). As antioxidative activity (AOA) is assumed to be a BBB characteristic, we tested whether AC improve AOA of EC. Monocultivated AC showed higher AOA [manganese superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (...

journal_title：FEBS letters

authors： Schroeter ML,Mertsch K,Giese H,Müller S,Sporbert A,Hickel B,Blasig IE

更新日期：1999-04-23 00:00:00

abstract：:Three compounds with cytokinin activity have been isolated from the medium of Rhodospirillum rubrum grown photosynthetically. Two N-6 aminopurine cytokinins revealed in the medium were identical with those obtained from R. rubrum cells previously. The third compound with cytokinin activity in Amaranthus caudatus bioas...

journal_title：FEBS letters

authors： Serdyuk OP,Smolygina LD,Muzafarov EN,Adanin VM,Arinbasarov MU

更新日期：1995-05-22 00:00:00

abstract：:Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the...

journal_title：FEBS letters

authors： Michel G,Rouillé Y,Chauvet MT,Chauvet J,Acher R

更新日期：1990-05-07 00:00:00

abstract：:The glycogen branching enzyme from Vibrio vulnificus (VvGBE) transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme (GBE). To elucidate the role of the N-domain of VvGBE in the unique branching pattern, domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE...

journal_title：FEBS letters

authors： Jo HJ,Park S,Jeong HG,Kim JW,Park JT

更新日期：2015-04-28 00:00:00

abstract：:The mitochondrial outer membrane contains a protein complex with at least eight subunits responsible for recognition and translocation of preproteins synthesized in the cytosol. Two subunits, the receptors Tom20 and Tom70, contain tetratrico peptide repeats that are thought to be involved in protein-protein interactio...

journal_title：FEBS letters

authors： Bömer U,Pfanner N,Dietmeier K

更新日期：1996-03-11 00:00:00

abstract：:Mitochondria continually fuse and divide to dynamically adapt to changes in metabolism and stress. Mitochondrial dynamics are also required for mitochondrial DNA (mtDNA) integrity; however, the underlying reason is not known. In this study, we examined the link between mitochondrial fusion and mtDNA maintenance in Sch...

journal_title：FEBS letters

authors： Delerue T,Khosrobakhsh F,Daloyau M,Emorine LJ,Dedieu A,Herbert CJ,Bonnefoy N,Arnauné-Pelloquin L,Belenguer P

更新日期：2016-10-01 00:00:00

abstract：:When acquiring internal membranes and vesicular transport, eukaryotic cells started to synthesize sphingolipids and sterols. The physical differences between these and the glycerophospholipids must have enabled the cells to segregate lipids in the membrane plane. Localizing this event to the Golgi then allowed them to...

journal_title：FEBS letters

authors： van Meer G,Hoetzl S

更新日期：2010-05-03 00:00:00

abstract：:The C-termini of p66 and p51 forms of HIV-1 reverse transcriptase have been engineered to contain a Glu-Glu-Phe sequence recognized by a monoclonal antibody to alpha-tubulin, YL1/2. Mutated RTs were purified in a single step using peptide elution from columns of immobilized YL1/2. The known sequence requirements of th...

journal_title：FEBS letters

authors： Stammers DK,Tisdale M,Court S,Parmar V,Bradley C,Ross CK

更新日期：1991-06-03 00:00:00

abstract：:Several mRNAs are known to be targeted to dendrites in hippocampal neurons. In this study, we show that brain-derived neurotrophic factor (BDNF) mRNA has two distinct cis-acting dendritic targeting elements in the short 3' untranslated region (UTR): a constitutive element and an activity-dependent one. Moreover, delet...

journal_title：FEBS letters

authors： Oe S,Yoneda Y

更新日期：2010-08-04 00:00:00

abstract：:Vesicular stomatitis virus (VSV) is a prototypic virus commonly used in studies of endocytosis and membrane trafficking. One proposed mechanism for VSV entry involves initial fusion with internal vesicles of multivesicular endosomes followed by back-fusion of these vesicles into the cytoplasm. One feature of endosomal...

journal_title：FEBS letters

authors： Roth SL,Whittaker GR

更新日期：2011-03-23 00:00:00

abstract：:Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development cor...

journal_title：FEBS letters

authors： Labhart P

更新日期：1994-12-19 00:00:00

abstract：:A cytochrome c (cyt. c) was solubilized with Triton-X-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of Rhodopseudomonas capsulata. Cyt. c and cytochrome oxidase were separated on Sephadex G-50 columns. Antibodies against cytochrome c2 from the same bacterium did not cross...

journal_title：FEBS letters

authors： Hüdig H,Drews G

更新日期：1983-02-21 00:00:00