Abstract:
:The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% identity to a C. elegans protein found in the database. Northern and Western blot analyses indicate that hSec10 has a broad tissue distribution. Immunofluorescence staining of COS cells cotransfected with hSec10p and a mammalian Sec8p demonstrates that these two proteins have an identical distribution in the cell including a localization in the peripheral cytoplasm. These data suggest that hSec10p is a component of the mammalian counterpart of the yeast Exocyst complex essential for post-Golgi traffic.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Guo W,Roth D,Gatti E,De Camilli P,Novick Pdoi
10.1016/s0014-5793(97)00109-9subject
Has Abstractpub_date
1997-03-10 00:00:00pages
135-9issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00109-9journal_volume
404pub_type
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