Identification of a third yeast mitochondrial Tom protein with tetratrico peptide repeats.


:The mitochondrial outer membrane contains a protein complex with at least eight subunits responsible for recognition and translocation of preproteins synthesized in the cytosol. Two subunits, the receptors Tom20 and Tom70, contain tetratrico peptide repeats that are thought to be involved in protein-protein interactions. We have identified Saccharomyces cerevisiae Tom72, a new Tom protein expressed at a low level. Tom72 is homologous to Tom 70, including seven tetratrico peptide repeats. Tom72 is targeted to the mitochondrial outer membrane, forms a large domain exposed to the cytosol and loosely associates with the translocase complex of the outer membrane. These results suggest that Tom72 represents a ninth, weakly expressed component of the preprotein translocase of the mitochondrial outer membrane.




FEBS letters


Bömer U,Pfanner N,Dietmeier K




Has Abstract


1996-03-11 00:00:00














  • Multicatalytic and 26 S ubiquitin/ATP-stimulated proteases in maturing rabbit red blood cells.

    abstract::Rabbit red blood cells of various ages were separated on Percoll gradients and the activities of two large cytosolic proteases were measured. Both the multicatalytic protease (MCP), assayed by hydrolysis of fluorigenic peptides, and the 26 S ubiquitin/ATP-stimulated protease, assayed by degradation of ubiquitin-lysozy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Di Cola D,Pratt G,Rechsteiner M

    更新日期:1991-03-11 00:00:00

  • Formation of free sphingosine and ceramide from exogenous ganglioside GM1 by cerebellar granule cells in culture.

    abstract::Cerebellar granule cells differentiated in culture were incubated with ganglioside [3H-Sph]GM1 in order to have it inserted into the plasma membrane and metabolized. Among the formed metabolites radioactive sphingosine and ceramide were identified. [3H]Ceramide started to be measurable after 10 min of incubation (puls...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Riboni L,Bassi R,Sonnino S,Tettamanti G

    更新日期:1992-03-30 00:00:00

  • Fatty acid acylation of membrane skeletal proteins in human erythrocytes.

    abstract::Fatty acid acylation of membrane proteins was studied on human erythrocytes by measuring incorporation of [3H]palmitate at different specific radioactivities. A 55 kDa polypeptide within the band 4.5 region was the main acceptor protein for acylation by fatty acids (palmitate, stearate, oleate), while other polypeptid...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Maretzki D,Mariani M,Lutz HU

    更新日期:1990-01-01 00:00:00

  • Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity.

    abstract::We explored the interactive effects of endothelial nitric oxide synthase (eNOS) genotypes and cigarette smoking on protein levels and enzyme activity in 33 postpartum placentas. Whilst the eNOS protein levels were lower in the rare allele (0.48+/-0.11, n=9 vs. 1. 05+/-0.10, n=24, P<0.01), the eNOS enzyme activity was ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang XL,Sim AS,Wang MX,Murrell GA,Trudinger B,Wang J

    更新日期:2000-04-07 00:00:00

  • The balance between pre- and post-transfer editing in tRNA synthetases.

    abstract::The fidelity of tRNA aminoacylation is dependent in part on amino acid editing mechanisms. A hydrolytic activity that clears mischarged tRNAs typically resides in an active site on the tRNA synthetase that is distinct from its synthetic aminoacylation active site. A second pre-transfer editing pathway that hydrolyzes ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Martinis SA,Boniecki MT

    更新日期:2010-01-21 00:00:00

  • Activation of Ca2+/calmodulin-dependent protein kinase II is involved in hyperosmotic induction of the human taurine transporter.

    abstract::We investigated the signaling pathways participating in hyperosmotic regulation of the human taurine transporter (TAUT) by using specific inhibitors of various intracellular signaling molecules. Among them, the specific inhibitor of calcium/calmodulin-dependent protein kinase II (Ca(2+)/CaM kinase II) completely repre...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Satsu H,Manabe M,Shimizu M

    更新日期:2004-07-02 00:00:00

  • Modulation of HIV-1 enhancer activity and virus production by cAMP.

    abstract::The effect of cAMP on the transcriptional activity of the HIV-1 long terminal repeat/enhancer was investigated and compared to the effect of cAMP on virus replication. In culture cAMP repressed virus replication in vivo using different cell types. Transient transfection studies with HIV-1 enhancer-derived luciferase r...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Banas B,Eberle J,Banas B,Schlöndorff D,Luckow B

    更新日期:2001-12-07 00:00:00

  • Change in lactate production in Myc-transformed cells precedes apoptosis and can be inhibited by Bcl-2 overexpression.

    abstract::As a result of Myc-dependent transcription of the LDH-A gene, Myc-transformed cells (Rat1-Myc) exhibit increased lactate production rates (LPR) even under aerobic conditions (the Warburg effect). Recently, the increased susceptibility to stress-induced apoptosis associated with Myc transfection has been linked to the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Papas KK,Sun L,Roos ES,Gounarides JS,Shapiro M,Nalin CM

    更新日期:1999-03-12 00:00:00

  • Reversal of oxidative phosphorylation in submitochondrial particles using glucose 6-phosphate and hexokinase as an ATP regenerating system.

    abstract::During steady-state, the Pi released in the medium is derived from glucose-6-phosphate which continuously regenerates the ATP hydrolyzed. A membrane potential (delta psi) can be built up in submitochondrial particles using glucose-6-phosphate and hexokinase as an ATP-regenerating system. The energy derived from the me...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: de Meis L,Grieco MA,Galina A

    更新日期:1992-08-17 00:00:00

  • Expression, purification and crystallization of fully active, glycosylated human interleukin-5.

    abstract::Recombinant human interleukin-5 (hIL-5) has been expressed at high levels and produced in large quantities in baculovirus infected Sf9 insect cells. The glycosylated protein was purified using immuno-affinity chromatography and gel filtration. Purified hIL-5 has been crystallized using standard vapour diffusion techni...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guisez Y,Oefner C,Winkler FK,Schlaeger EJ,Zulauf M,Van der Heyden J,Plaetinck G,Cornelis S,Tavernier J,Fiers W

    更新日期:1993-09-27 00:00:00

  • Anti-cytokine autoantibodies are ubiquitous in healthy individuals.

    abstract::Anti-cytokine autoantibodies in healthy individuals have been widely reported but the occurrence is variable and inconstant. We hypothesized that cytokine-binding in vivo may explain their variable and infrequent detection. Therefore, we focused on the detection of the cytokine-autoantibody complexes and found that an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Watanabe M,Uchida K,Nakagaki K,Kanazawa H,Trapnell BC,Hoshino Y,Kagamu H,Yoshizawa H,Keicho N,Goto H,Nakata K

    更新日期:2007-05-15 00:00:00

  • New muteins of RNase A with enhanced antitumor action.

    abstract::Monomeric bovine pancreatic RNase A has been transformed into a dimeric ribonuclease with antitumor activity (Di Donato, A., Cafaro, V. and D'Alessio, G. (1994) J. Biol. Chem. 269, 17394-17396). This was accomplished by replacing the residues located in the RNase chain at positions 19, 28, 31, and 32, with proline, le...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cafaro V,Bracale A,Di Maro A,Sorrentino S,D'Alessio G,Di Donato A

    更新日期:1998-10-16 00:00:00

  • Le couplage phosphore-phosphore dans l'adenosine di- et triphosphate.

    abstract::The pH dependence of spin-spin coupling constants J (P-O-P) of ADP and ATP has been determined from the phosphorus NMR spectra at 0 degrees. The variation curves have been interpreted as titration curves. These give different pK values. Comparison with the tripolyphosphate shows the existence of triphosphate chain str...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ellenberger M,Brehamet L,Villemin M,Toma F

    更新日期:1970-06-01 00:00:00

  • Linking entries in protein interaction database to structured text: the FEBS Letters experiment.

    abstract::The corpus of the scientific literature has reached such size that a lot of useful data, dispersed throughout millions different articles, are now hard to recover. For instance, many articles in the biological domain describe relationships between entities (gene, proteins, small molecules, etc.) yet this crucial infor...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Ceol A,Chatr-Aryamontri A,Licata L,Cesareni G

    更新日期:2008-04-09 00:00:00

  • Inhibition of the mitochondrial cyclosporin A-sensitive permeability transition pore by the arginine reagent phenylglyoxal.

    abstract::The mitochondrial permeability transition pore, a cyclosporin A-sensitive channel, is controlled by the transmembrane electric potential difference across the inner membrane. Here, we show that treatment of rat liver mitochondria with the arginine reagent phenylglyoxal inhibits the permeability transition pore trigger...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eriksson O,Fontaine E,Petronilli V,Bernardi P

    更新日期:1997-06-16 00:00:00

  • alpha-Tomatine, the major saponin in tomato, induces programmed cell death mediated by reactive oxygen species in the fungal pathogen Fusarium oxysporum.

    abstract::The tomato saponin alpha-tomatine has been proposed to kill sensitive cells by binding to cell membranes followed by leakage of cell components. However, details of the modes of action of the compound on fungal cells are poorly understood. In the present study, mechanisms involved in alpha-tomatine-induced cell death ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ito S,Ihara T,Tamura H,Tanaka S,Ikeda T,Kajihara H,Dissanayake C,Abdel-Motaal FF,El-Sayed MA

    更新日期:2007-07-10 00:00:00

  • Molecular diagnosis of transthyretin Met30 mutation in an Italian family with familial amyloidotic polyneuropathy.

    abstract::We report the molecular analysis of the transthyretin gene in a large Italian pedigree with familial amyloidotic polyneuropathy and demonstrate the presence of a Met30 mutation. The usefulness of the genetic analysis in the identification of presymptomatic persons and the diagnosis of individuals with partial symptoms...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Strocchi P,Tang XM,Cambi F

    更新日期:1995-02-13 00:00:00

  • Tyrosine phosphorylation of human urokinase-type plasminogen activator.

    abstract::Immunoblotting analysis of purified human urokinase plasminogen activator (u-PA), gives a positive signal when reacted with anti-phosphotyrosine monoclonal antibodies (MoAb anti-P-Tyr); competition with o-phospho-DL-tyrosine (P-Tyr) but not o-phospho-DL-threonine or serine (P-Treo, P-Ser) completely suppresses this si...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Barlati S,Paracini F,Bellotti D,De Petro G

    更新日期:1991-04-09 00:00:00

  • The expression of the major shed Trypanosoma cruzi antigen results from the developmentally-regulated transcription of a small gene family.

    abstract::To better understand the mechanisms involved in the developmental expression of Trypanosoma cruzi antigens we examined the gene structure and transcription properties of the major shed trypomastigote antigen (SAPA). We report in this paper that SAPA is encoded by a small family of at lest 6 genes which differ mainly i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Affranchino JL,Pollevick GD,Frasch AC

    更新日期:1991-03-25 00:00:00

  • Heterogeneity of water-soluble amyloid beta-peptide in Alzheimer's disease and Down's syndrome brains.

    abstract::Water-soluble amyloid beta-peptides (sA beta), ending at residue 42, precede amyloid plaques in Down's syndrome (DS). Here we report that sA beta consists of the full-length A beta(1-42) and peptides truncated and modified by cyclization of the N-terminal glutamates, A beta[3(pE)-42] and A beta[11(pE)-42]. The A beta[...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Russo C,Saido TC,DeBusk LM,Tabaton M,Gambetti P,Teller JK

    更新日期:1997-06-16 00:00:00

  • Zn2+ enhances protein tyrosine kinase activity of human platelet membranes.

    abstract::In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Findik D,Presek P

    更新日期:1988-08-01 00:00:00

  • Mechanisms of the inhibition of reverse transcription by unmodified and modified antisense oligonucleotides.

    abstract::We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides prevent cDNA synthesis by AMV or HIV reverse transcriptases. Antisense oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA fragment bound to the antisense oligonucleotide by t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hatta T,Kim SG,Nakashima H,Yamamoto N,Sakamoto K,Yokoyama S,Takaku H

    更新日期:1993-09-13 00:00:00

  • Molecular electroporation: a unifying concept for the description of membrane pore formation by antibacterial peptides, exemplified with NK-lysin.

    abstract::The antibacterial activity of many small, positively charged peptides and proteins is based on pore formation in lipid bilayers. It is here proposed to arise from an electroporation effect. This hypothesis is supported by calculations of the electrostatic potential of NK-lysin associated to a membrane. For a significa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Miteva M,Andersson M,Karshikoff A,Otting G

    更新日期:1999-11-26 00:00:00

  • Modulation of phosphoinositide 3-kinase activation by cholesterol level suggests a novel positive role for lipid rafts in lysophosphatidic acid signalling.

    abstract::Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peres C,Yart A,Perret B,Salles JP,Raynal P

    更新日期:2003-01-16 00:00:00

  • Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans.

    abstract::Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glyc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sugihara K,Shibata TK,Takata K,Kimura T,Kanayama N,Williams R,Hatakeyama S,Akama TO,Kuo CW,Khoo KH,Fukuda MN

    更新日期:2013-10-01 00:00:00

  • Localization of myosin IIB at the leading edge of growth cones from rat dorsal root ganglionic cells.

    abstract::Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cheng TP,Murakami N,Elzinga M

    更新日期:1992-10-19 00:00:00

  • Ceruloplasmin and the reactions forming diferric transferrin.

    abstract::The rate of formation of diferric-transferrin has been studied using various combinations of Fe(II), Tf, Cp and h serum. When the reactants were added in a correct physiological sequence, ceruloplasmin and diluted human serum showed the fastest rate of saturation of transferrin. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chidambaram MV,Barnes G,Frieden E

    更新日期:1983-08-08 00:00:00

  • alpha-Allenyl putrescine, an enzyme-activated irreversible inhibitor of bacterial and mammalian ornithine decarboxylases.

    abstract::alpha-Allenyl putrescine (5,6-heptadiene-1,4-diamine) was designed as a new potential enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC). This compound, and more specifically its (R)-enantiomer, produced time-dependent inhibitions of E. coli and rat liver ODC. The inhibitions exhibit saturation k...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Danzin C,Casara P

    更新日期:1984-09-03 00:00:00

  • Differential expression and anti-oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization.

    abstract::Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Xu X,Leng JY,Gao F,Zhao ZA,Deng WB,Liang XH,Zhang YJ,Zhang ZR,Li M,Sha AG,Yang ZM

    更新日期:2014-05-02 00:00:00

  • Protein syntheis in brain mitochondrial and synaptosomal preparations.

    abstract::The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Morgan IG

    更新日期:1970-10-16 00:00:00