Abstract:
:The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs) in the cytoplasm, followed by extracytoplasmic maturation. By using cell fractionation, protein-protein interaction, and in vivo labeling assays we obtained evidence that, in P. aeruginosa, pyoverdine NRPSs assemble with precursor-generating enzymes into a membrane-bound multi-enzymatic complex, for which we propose the name "siderosome". The pyoverdine biogenetic complex represents a novel example of subcellular compartmentalization of a secondary metabolic pathway in prokaryotes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Imperi F,Visca Pdoi
10.1016/j.febslet.2013.08.039subject
Has Abstractpub_date
2013-11-01 00:00:00pages
3387-91issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00668-6journal_volume
587pub_type
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