Abstract:
:We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B(12) activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coalpha-[alpha-(7-adenyl)]-Cobeta-cyanocobamide or pseudovitamin B(12). This molecule differs from cobalamin in the alpha-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a alpha-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B(12).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Santos F,Vera JL,Lamosa P,de Valdez GF,de Vos WM,Santos H,Sesma F,Hugenholtz Jdoi
10.1016/j.febslet.2007.09.012subject
Has Abstractpub_date
2007-10-16 00:00:00pages
4865-70issue
25eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00983-0journal_volume
581pub_type
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