Extreme thermostability of tarantula hemocyanin.

abstract：:Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning (1)H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphoc...

journal_title：FEBS letters

authors： Griffin JL,Mann CJ,Scott J,Shoulders CC,Nicholson JK

更新日期：2001-12-07 00:00:00

abstract：:Insulin receptors (IRs) that are truncated at the end of the ectodomain form dimers that bind insulin with different characteristics to wild type receptors. These soluble IRs have lowered affinity for insulin compared with full-length IR, and exhibit linear Scatchard plots in contrast to the curvilinear plots obtained...

journal_title：FEBS letters

authors： Hoyne PA,Cosgrove LJ,McKern NM,Bentley JD,Ivancic N,Elleman TC,Ward CW

更新日期：2000-08-11 00:00:00

abstract：:Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase...

journal_title：FEBS letters

authors： Heldman E,Balan A,Horowitz O,Ben-Zion S,Torten M

更新日期：1985-01-28 00:00:00

abstract：:Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-tri...

journal_title：FEBS letters

authors： Dehio M,Gómez-Duarte OG,Dehio C,Meyer TF

更新日期：1998-03-06 00:00:00

abstract：:A new insertion sequence, IS186, has been identified from Escherichia coli and sequenced. It contains 1336 base pairs with a terminal inverted repeat of 22 nucleotides. A long open reading frame, from an ATG codon at position 55 to a TAG termination codon at 1327, could code for a polypeptide of 424 amino acids. This ...

journal_title：FEBS letters

authors： Chong P,Hui I,Loo T,Gillam S

更新日期：1985-11-11 00:00:00

abstract：:With the ultimate aim of developing an effective antiviral strategy against HIV-1, a mono-DNA enzyme possessing the 10-23 catalytic motif [Santoro and Joyce (1997) Proc. Natl. Acad. Sci. USA 94, 4264-4266] was synthesized against the HIV-1 envelope gene. We tested the in vitro cleavage efficiency of the 178 bp long tr...

journal_title：FEBS letters

authors： Dash BC,Harikrishnan TA,Goila R,Shahi S,Unwalla H,Husain S,Banerjea AC

更新日期：1998-07-24 00:00:00

abstract：:Vascular smooth muscle cell (VSMC) proliferation plays a key role in neointimal hyperplasia and restenosis. Here we report the role of the microRNA miR-142-5p and its downstream target genes on the proliferation of cultured VSMCs. miR-142-5p promoted VSMC proliferation by down-regulating B cell translocation gene 3 (B...

journal_title：FEBS letters

authors： Kee HJ,Park S,Kwon JS,Choe N,Ahn Y,Kook H,Jeong MH

更新日期：2013-08-02 00:00:00

abstract：:At alpha 1-adrenergic receptors in isolated rat liver parenchymal cells, (-)-epinephrine is potent in eliciting a maximal increase in glycogenolysis (Kact = 24 nM). This contrasts with a 100-fold lower affinity for the agonist at alpha 1-adrenergic receptors of intact hepatocytes determined from equilibrium competitio...

journal_title：FEBS letters

authors： Schwarz KR,Lanier SM,Carter EA,Graham RM,Homcy CJ

更新日期：1985-08-05 00:00:00

abstract：:Using the patch-clamp technique, we demonstrate here the opening of K+ channels evoked by the actively transported amino acid L-alanine in isolated Necturus enterocytes. These channels had a conductance of about 30 pS and their activation was dependent on transmembrane electrical potential and cytosolic Ca2+. ...

journal_title：FEBS letters

authors： Sheppard DN,Giraldez F,Sepúlveda FV

更新日期：1988-07-18 00:00:00

abstract：:Membrane incorporation and aggregation of the peptide alamethicin have been investigated as a function of lipid type. Head group and acyl chain regions both contribute to modulate alamethicin incorporation. Specifically, the peptide prefers thin membranes and saturated chains; incorporation is reduced by the presence ...

journal_title：FEBS letters

authors： Stankowski S,Schwarz G

更新日期：1989-07-03 00:00:00

abstract：:Nuclear magnetic resonance (NMR) spectroscopy was used to determine the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2 (hSCP2), which is a basic protein with 123 residues believed to participate in the intracellular transport of cholesterol and various oth...

journal_title：FEBS letters

authors： Szyperski T,Scheek S,Johansson J,Assmann G,Seedorf U,Wüthrich K

更新日期：1993-11-29 00:00:00

abstract：:A high-potential iron-sulfur cluster with characteristics similar to a Rieske-type center was detected in the plasma membrane of Sulfolobus acidocaldarius by EPR spectroscopy. In the reduced form this center has g-values of gz = 2.031, gy = 1.890 and gx = 1.725 (gav = 1.88, rhombicity = 0.37) and its reduction potenti...

journal_title：FEBS letters

authors： Anemüller S,Schmidt CL,Schäfer G,Teixeira M

更新日期：1993-02-22 00:00:00

abstract：:VacA is a major virulence factor of the widespread stomach-dwelling bacterium Helicobacter pylori. It causes cell vacuolation and tissue damage by forming anion-selective, urea-permeable channels in plasma and endosomal membranes. We report that several flavone derivatives and other polyphenols present in vegetables a...

journal_title：FEBS letters

authors： Tombola F,Campello S,De Luca L,Ruggiero P,Del Giudice G,Papini E,Zoratti M

更新日期：2003-05-22 00:00:00

abstract：:Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lack...

journal_title：FEBS letters

authors： Abe Y,Goda W,Ikeshima-Kataoka H,Yasui M

更新日期：2017-12-01 00:00:00

abstract：:The assembly of cytochrome c oxidase subunits I-III was studied in vitro in isolated rat liver mitochondria pre-labeled with [35S]methionine. Individual subunits were immunoabsorbed with monospecific antibodies. Isolated heme a from rat liver mitochondria, when added to radiolabeled mitochondria, induced assembly of s...

journal_title：FEBS letters

authors： Wielburski A,Nelson BD

更新日期：1984-11-19 00:00:00

abstract：:In bovine adrenocortical cells, the fatty acyl chains of the phospholipids have been identified in the membranes of the different cell compartments: plasma membranes, Golgi complex and coated vesicle membranes. An increase in the total number of unsaturation in the fatty acid is demonstrated in the coated vesicle memb...

journal_title：FEBS letters

authors： de Paillerets C,Bomsel M,Weintraub H,Pépin D,Alfsen A

更新日期：1987-07-13 00:00:00

abstract：:The vertebrate transcription factor CTCF is not only involved in transcriptional activation, insulation and genomic imprinting, but also in transcriptional repression. Sequence motifs mediating these activities have not been identified so far. We have mapped a short repression motif to residues 150-170 within the N-te...

journal_title：FEBS letters

authors： Drueppel L,Pfleiderer K,Schmidt A,Hillen W,Berens C

更新日期：2004-08-13 00:00:00

abstract：:32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical...

journal_title：FEBS letters

authors： Ghosh RK,Guhathakurta I

更新日期：1983-10-03 00:00:00

abstract：:The CO-binding reaction of cytochrome P-450cam bound with (1R)-camphor and (1S)-camphor are compared in the temperature region of 210-260 K using time-resolved Fourier-transform infrared spectroscopy with the CO stretch vibration as spectroscopic probe. For (1S)-camphor as substrate the association of CO is slowed dow...

journal_title：FEBS letters

authors： Contzen J,Ristau O,Jung C

更新日期：1996-03-25 00:00:00

abstract：:We previously identified and purified a nucleolar phosphoprotein, nucleophosmin/B23, as a stimulatory factor for replication from the adenovirus chromatin. We show here that nucleophosmin/B23 functions as a histone chaperone protein such as nucleoplasmin, TAF-I, and NAP-I. Nucleophosmin/B23 was shown to bind to histon...

journal_title：FEBS letters

authors： Okuwaki M,Matsumoto K,Tsujimoto M,Nagata K

更新日期：2001-10-12 00:00:00

abstract：:A single copy of a glucocorticoid-responsive element (GRE) is sufficient in mediating the combinatorial response of a promoter to both glucocorticoids and insulin in HepG2 cells. This requires the presence of active glucocorticoid receptor (GR) since the response is significantly inhibited by the anti-glucocorticoid R...

journal_title：FEBS letters

authors： Georgakopoulos A,Tsawdaroglou N

更新日期：1996-03-04 00:00:00

abstract：:The products of the isiAB operon are a chlorophyll antenna protein (IsiA) and flavodoxin (IsiB), which accumulate in cyanobacteria grown under iron starvation conditions. Here we show that strong light triggers de-repression of isiAB transcription and leads to IsiA and flavodoxin accumulation under iron replete condit...

journal_title：FEBS letters

authors： Havaux M,Guedeney G,Hagemann M,Yeremenko N,Matthijs HC,Jeanjean R

更新日期：2005-04-25 00:00:00

abstract：:A rapid and highly efficient method of purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been developed. This method relies upon a six-histidine affinity tag fused to the C-terminus of subunit I, which confers to the oxidase a high affinity for Ni(2+)-nitrilotriacetic acid (NTA) agarose. The...

journal_title：FEBS letters

authors： Mitchell DM,Gennis RB

更新日期：1995-07-10 00:00:00

abstract：:Sequence comparison based on Hydrophobic Cluster Analysis procedures shows that the extracellular approximately 200 amino acids domains of cytokines receptors belonging to the Cytokine/Growth hormone/Prolactin receptor family and to the Interferon one are organized in two homologous subdomains. Further, comparison of ...

journal_title：FEBS letters

authors： Thoreau E,Petridou B,Kelly PA,Djiane J,Mornon JP

更新日期：1991-04-22 00:00:00

abstract：:Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...

journal_title：FEBS letters

authors： Yu J,Jia Y,Guo Y,Chang G,Duan W,Sun M,Li B,Li C

更新日期：2010-07-02 00:00:00

abstract：:Over the past several years, the study of a hereditary tumor syndrome, tuberous sclerosis complex (TSC), has shed light on the regulation of cellular proliferation and growth. TSC is an autosomal dominant disorder that is due to inactivating mutations in TSC1 or TSC2 and characterized by benign tumors (hamartomas) inv...

journal_title：FEBS letters

authors： Han JM,Sahin M

更新日期：2011-04-06 00:00:00

abstract：:The transcriptional start site of the human cholecystokinin (CCK)-A receptor gene was determined by the Capsite Hunting method. Two sequence changes were detected, a G to T change in nucleotide -128, and an A to G change in nucleotide -81. The homozygote (T/T, G/G) was detected in 25 of 1296 individuals (1.9%) in the ...

journal_title：FEBS letters

authors： Funakoshi A,Miyasaka K,Matsumoto H,Yamamori S,Takiguchi S,Kataoka K,Takata Y,Matsusue K,Kono A,Shimokata H

更新日期：2000-01-28 00:00:00

abstract：:Rho-associated kinase 2 (Rock2) is known to promote tumorigenesis in hepatocellular carcinoma (HCC). CCAAT/enhancer-binding protein delta (CEBPD) functions as a tumor suppressor. In this study, we found that the expression of Rock2 and CEBPD are inversely correlated. Knockdown of Rock2 increased CEBPD expression and i...

journal_title：FEBS letters

authors： Li M,Zhou W,Yuan R,Chen L,Liu T,Huang D,Hao L,Xie Y,Shao J

更新日期：2015-04-13 00:00:00

abstract：:A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility...

journal_title：FEBS letters

authors： Zlatanov IV,Foley M,Birmingham J,Garland PB

更新日期：1987-09-28 00:00:00

abstract：:Limb girdle muscular dystrophy type 2A is linked to a skeletal muscle-specific calpain isoform known as p94. Isolation of the intact 94-kDa enzyme has been difficult to achieve due to its rapid autolysis, and uncertainty has arisen over its Ca2+-dependence for activity. We have expressed a C-terminally truncated form ...

journal_title：FEBS letters

authors： Rey MA,Davies PL

更新日期：2002-12-18 00:00:00