Abstract:
:32P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30-60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphatidylinositol (MPI) at 30 s, reflecting the activation of the MPI cycle via the cytosolic phospholipase C; (2) an enhancement of phosphatidylinositol-4-phosphate (DPI) and phosphatidylinositol-4,5-bisphosphate (TPI) labelling at later incubation times; (3) an early degradation of TPI with a counterbalancing formation of DPI. The latter changes suggest a receptor-mediated stimulation of TPI-phosphomonoesterase, the role of which in the mechanism of platelet activation is discussed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mauco G,Chap H,Douste-Blazy Ldoi
10.1016/0014-5793(83)80643-7subject
Has Abstractpub_date
1983-03-21 00:00:00pages
361-5issue
2eissn
0014-5793issn
1873-3468journal_volume
153pub_type
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