Fatty acid 9- and 13-hydroperoxide lyases from cucumber.

abstract：:Prepro-vasoactive intestinal peptide (prepro VIP) was expressed in NIH 3T3 cells, and the prepro VIP-derived peptides produced by the cells were analyzed by chromatography combined with sequence-specific radio-immunoanalysis. In accordance with what has previously been reported on processing in non-endocrine cell line...

journal_title：FEBS letters

authors： Wulff BS,Georg B,Fahrenkrug J

更新日期：1994-03-14 00:00:00

abstract：:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

journal_title：FEBS letters

authors： Li W,Chen L,Fan W,Feng C

更新日期：2012-01-20 00:00:00

abstract：:The 108-residue protein E. coli thioredoxin has been uniformly enriched to 50% with deuterium at all carbon-bound hydrogen positions. Isotropic mixing (i.e. TOCSY) experiments have been conducted for both the deuterated and natural-abundance samples. Using a 54 ms mixing time correlation peaks can be seen for all four...

journal_title：FEBS letters

authors： LeMaster DM

更新日期：1988-06-20 00:00:00

abstract：:We show here that phosphorylation of the insulin receptor and insulin receptor substrate-1 is increased when suspended cells are replated on fibronectin. This is not due to decreased numbers of cell surface receptors, alteration of insulin binding, or stimulation of a phosphatase activity in non-adherent cells. Expres...

journal_title：FEBS letters

authors： El Annabi S,Gautier N,Baron V

更新日期：2001-11-02 00:00:00

abstract：:Acid extracts of rat pituitary neuro-intermediate lobes have been shown by ion-exchange chromatography and radio-immunoassay to contain predominantly the inactive derivatives of beta-endorphin, alpha, N-acetyl beta-endorphin 1-27 and alpha, N-acetyl beta-endorphin 1-26; the biologically active form, beta-endorphin 1-3...

journal_title：FEBS letters

authors： Ham J,Smyth DG

更新日期：1984-10-01 00:00:00

abstract：:Vitamin D3, cholecalciferol, induces cell-cell communication via gap junctions in murine fibroblasts (C3H/10T 1/2 cells) at concentrations between 0.01 and 1.0 microM, as assayed by the dye transfer method using Lucifer yellow CH. The extent of induction is similar to that obtained with the positive controls, canthaxa...

journal_title：FEBS letters

authors： Stahl W,Nicolai S,Hanusch M,Sies H

更新日期：1994-09-19 00:00:00

abstract：:A number of recent observations have suggested that the endocytic and biosynthetic pathways may share fundamentally similar transport mechanisms at the molecular level. Some of the more striking of these suggestions have come from a comparison of the effects of the macrocyclic lactone brefeldin A (BFA) on endosomes an...

journal_title：FEBS letters

authors： Hunziker W,Whitney JA,Mellman I

更新日期：1992-07-27 00:00:00

abstract：:We have constructed cDNA clones covering the entire coding region of mouse, human and rabbit preprocathepsin K mRNA for studies on bone turnover. The clone pMCatK-1 for mouse cathepsin K shares 87% nucleotide homology with the corresponding human and rabbit sequences. Analysis of a panel of mouse tissues for tissue di...

journal_title：FEBS letters

authors： Rantakokko J,Aro HT,Savontaus M,Vuorio E

更新日期：1996-09-16 00:00:00

abstract：:Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy...

journal_title：FEBS letters

authors： Engels F,Willems H,Nijkamp FP

更新日期：1986-12-15 00:00:00

abstract：:Complex I (NADH:ubiquinone oxidoreductase) is the first and largest protein complex of the oxidative phosphorylation. Crystal structures have elucidated the positions of most subunits of bacterial evolutionary origin in the complex, but the positions of the eukaryotic subunits are unknown. Based on the analysis of seq...

journal_title：FEBS letters

authors： Szklarczyk R,Wanschers BF,Nabuurs SB,Nouws J,Nijtmans LG,Huynen MA

更新日期：2011-03-09 00:00:00

abstract：:We previously found a brain-specific glycoprotein in the rat brain. It postnatally increases and is rich in the mature brain. We cloned cDNA of this protein. It is composed of a signal peptide, a V-type immunoglobulin domain, two C1-type immunoglobulin domains, a transmembrane segment and a cytoplasmic region containi...

journal_title：FEBS letters

authors： Sano S,Ohnishi H,Omori A,Hasegawa J,Kubota M

更新日期：1997-07-14 00:00:00

abstract：:Intracellular Ca(2+) ([Ca(2+)](i)) changes were measured in cell bodies of cultured rat hippocampal neurones with the fluorescent indicator Fluo-3. In the absence of external Ca(2+), the cholinergic agonist carbachol (200 microM) and the sarcoendoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (0.4 microM) both t...

journal_title：FEBS letters

authors： Bouron A

更新日期：2000-03-31 00:00:00

abstract：:Apoptosis is a physiological process for active cell removal. One of its hallmarks is an increased cytosolic Ca2+ content. Several genes involved in apoptosis control have been identified, but their mode of action is not understood in detail. Apoptosis may relate to oncogenesis, in that some malignant tumors may grow ...

journal_title：FEBS letters

authors： Richter C

更新日期：1993-06-28 00:00:00

abstract：:The interaction between two codon-anticodon duplexes of the ribosomal A- and P-site-bound tRNAs is the key feature of the proposed model. This interaction prohibits non-canonical base pairing at the first and second positions of the codon and controls base pairing at the third position (wobbling rules ensuing from the...

journal_title：FEBS letters

authors： Lim VI,Venclovas C

更新日期：1992-11-23 00:00:00

abstract：:In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; howe...

journal_title：FEBS letters

authors： Ogasawara Y,Namai T,Yoshino F,Lee MC,Ishii K

更新日期：2007-05-29 00:00:00

abstract：:Auxin increases phospholipase A(2) activity within 2min (Paul, R., Holk, A. and Scherer, G.F.E. (1998) Fatty acids and lysophospholipids as potential second messengers in auxin action. Rapid activation of phospholipase A(2) activity by auxin in suspension-cultured parsley and soybean cells. Plant J. 16, 601-611) and t...

journal_title：FEBS letters

authors： Scherer GF,Zahn M,Callis J,Jones AM

更新日期：2007-09-04 00:00:00

abstract：:As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-...

journal_title：FEBS letters

authors： Syomin BV,Kandror KV,Semakin AB,Tsuprun VL,Stepanov AS

更新日期：1993-06-01 00:00:00

abstract：:The cytosolic peptidyl-prolyl cis/trans isomerase cyclophilin from pig kidney can accelerate catalytically the cis/trans isomerization of prolyl peptide bonds. One- and two-dimensional 1H NMR spectroscopy was used to prove that the polypeptide hormone calcitonin is a substrate for cyclophilin. Isomerization of only on...

journal_title：FEBS letters

authors： Kern D,Drakenberg T,Wikström M,Forsén S,Bang H,Fischer G

更新日期：1993-06-01 00:00:00

abstract：:The monoclonal antibody 791T /36, prepared against a human osteogenic sarcoma cell line, 791T , reacts with a variety of human tumours and also mitogen-stimulated PBMN cells. The target antigen as expressed upon 791T cells is a monomeric plasma membrane-associated glycoprotein with an apparent Mr of 72000. By quantita...

journal_title：FEBS letters

authors： Price MR,Campbell DG,Robins RA,Blecher TE,Baldwin RW

更新日期：1984-06-04 00:00:00

abstract：:Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray a...

journal_title：FEBS letters

authors： Hagiwara K,Katsuda T,Gailhouste L,Kosaka N,Ochiya T

更新日期：2015-12-21 00:00:00

abstract：:Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

journal_title：FEBS letters

authors： Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

更新日期：1988-06-20 00:00:00

abstract：:Apoptosis represents a fundamental process during fetal/post-natal testis development. Therefore pro- and anti-apoptotic proteins are essential to regulate testis physiology. c-Flip(L) is a known inhibitor of caspase 8/10 activity; in this study its perinatal expression in mouse male germ cells was investigated. In te...

journal_title：FEBS letters

authors： Giampietri C,Petrungaro S,Coluccia P,Antonangeli F,Paone A,Padula F,De Cesaris P,Ziparo E,Filippini A

更新日期：2006-11-13 00:00:00

abstract：:The aspA gene, encoding the aspartyl-tRNA synthetase (AspRS) from the hyperthermophilic archaeon Pyrococcus sp. KOD1, was expressed in Escherichia coli. The KOD1 AspRS, which was purified to homogeneity and was shown to be functional in dimeric form, aminoacylated tRNA from KOD1. The optimum temperature for this activ...

journal_title：FEBS letters

authors： Fujiwara S,Lee SG,Haruki M,Kanaya S,Takagi M,Imanaka T

更新日期：1996-09-23 00:00:00

abstract：:NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...

journal_title：FEBS letters

authors： Drincovich MF,Casati P,Andreo CS

更新日期：2001-02-09 00:00:00

abstract：:Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with re...

journal_title：FEBS letters

authors： Misra HS,Khairnar NP,Barik A,Indira Priyadarsini K,Mohan H,Apte SK

更新日期：2004-12-03 00:00:00

abstract：:The 5'-untranslated region (5'-UTR) sequences of 33 GB virus C/hepatitis G virus (GBV-C/HGV) obtained from different geographic areas were determined through reverse-transcription polymerase chain reaction and dideoxy chain termination sequencing, the alignment of sequences, the estimation of the number of nucleotide ...

journal_title：FEBS letters

authors： Mukaide M,Mizokami M,Orito E,Ohba K,Nakano T,Ueda R,Hikiji K,Iino S,Shapiro S,Lahat N,Park YM,Kim BS,Oyunsuren T,Rezieg M,Al-Ahdal MN,Lau JY

更新日期：1997-04-21 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP c...

journal_title：FEBS letters

authors： Jarmuszkiewicz W,Antos N,Swida A,Czarna M,Sluse FE

更新日期：2004-07-02 00:00:00

abstract：:We have investigated the functional importance of nucleotide sequence differences between proximal (P-) and distal (D-) p53-binding elements in the MCK promoter. P- and D-elements normally co-operate to permit synergistic promoter activation by p53. Interestingly, we find that P-elements cannot co-operate with each ot...

journal_title：FEBS letters

authors： Jackson P,Yardley G

更新日期：1997-04-14 00:00:00

abstract：:The atomic structures of K+ channels have added a new dimension to our understanding of K+ channel function. I will briefly review how structures have influenced our views on ion conduction, gating of the pore, and voltage sensing. ...

journal_title：FEBS letters

authors： MacKinnon R

更新日期：2003-11-27 00:00:00

abstract：:Clusters of codons pairing to low-abundance tRNAs synchronize the translation with co-translational folding of single domains in multidomain proteins. Although proven with some examples, the impact of the ribosomal speed on the folding and solubility on a global, cell-wide level remains elusive. Here we show that upre...

journal_title：FEBS letters

authors： Fedyunin I,Lehnhardt L,Böhmer N,Kaufmann P,Zhang G,Ignatova Z

更新日期：2012-09-21 00:00:00