Abstract:
:Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), a byproduct of the formation of thromboxane A2. 9-HODE and HHT were the most prominent products formed under the incubation conditions used. These products were formed via a cyclooxygenase-catalyzed reaction, since their formation was inhibited by 1 microM indomethacin. Thus, under basal conditions AM possess substantial cyclooxygenase activity. The biological significance of 9-HODE and related substances is discussed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Engels F,Willems H,Nijkamp FPdoi
10.1016/0014-5793(86)81121-8subject
Has Abstractpub_date
1986-12-15 00:00:00pages
249-53issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(86)81121-8journal_volume
209pub_type
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