Protein NMR resonance assignment by isotropic mixing experiments on random fractionally deuterated samples.

abstract：:We have shown that OSCP, a subunit of yeast mitochondrial ATP synthase, can be incorporated into the intact enzyme as a fusion protein representing OSCP fused at its C-terminus to the green fluorescent protein (GFP) of Aequorea victoria. The relevant fusion OSCP-GFP-h6 additionally contains a hexahistidine tag at the ...

journal_title：FEBS letters

authors： Prescott M,Lourbakos A,Bateson M,Boyle G,Nagley P,Devenish RJ

更新日期：1997-07-07 00:00:00

abstract：:Enzymes from extremely halophilic archaea are readily denatured in the absence of a high salt concentration. However, we have observed here that a nucleoside diphosphate kinase prepared from Halobacterium salinarum was active and stable in the absence of salt, though it has the amino acid composition characteristic of...

journal_title：FEBS letters

authors： Ishibashi M,Tokunaga H,Hiratsuka K,Yonezawa Y,Tsurumaru H,Arakawa T,Tokunaga M

更新日期：2001-03-30 00:00:00

abstract：:Two highly contrasted images depict genomes: at first sight, genes appear to be distributed randomly along the chromosome. In contrast, their organisation into operons (or pathogenicity islands) suggests that, at least locally, related functions are in physical proximity. Analysis of the codon usage bias in orthologou...

journal_title：FEBS letters

authors： Rocha EP,Sekowska A,Danchin A

更新日期：2000-06-30 00:00:00

abstract：:Heat shock proteins (Hsps) are induced in response to various kinds of environmental and physiological stresses. However, it is unclear whether Hsps play roles in protecting cells in the digestive organs against xenobiotic chemicals. Here, we found that feeding induces expression of a set of Hsps specifically in the m...

journal_title：FEBS letters

authors： Katsuki K,Fujimoto M,Zhang XY,Izu H,Takaki E,Tanizawa Y,Inouye S,Nakai A

更新日期：2004-07-30 00:00:00

abstract：:Activation of T lymphocytes results in an increase in intracellular Ca2+ due in large part to influx of extracellular Ca2+. Using the patch clamp technique, an inward current in Jurkat T lymphocytes was observed upon depolarization from a holding potential of -90 mV but not from -60 mV. This whole-cell current was ins...

journal_title：FEBS letters

authors： Densmore JJ,Szabo G,Gray LS

更新日期：1992-11-09 00:00:00

abstract：:Persistent tumour necrosis factor alpha (TNF-alpha) exposure uncouples proximal T-cell receptor (TCR)-signalling events. Here, we demonstrate that chronic TNF-alpha exposure also attenuates signalling distal to the TCR, by specifically inhibiting Ca2+ influx evoked by thapsigargin in CD4+ T-cells. Mitogen-induced Ca2+...

journal_title：FEBS letters

authors： Church LD,Goodall JE,Rider DA,Bacon PA,Young SP

更新日期：2005-02-28 00:00:00

abstract：:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and e...

journal_title：FEBS letters

authors： Baram D,Yonath A

更新日期：2005-02-07 00:00:00

abstract：:Analysis of glutamic acid decarboxylase (GDC) (EC 4.1.1.15) from Escherichia coli ATCC 11246 revealed the presence of six pyridoxal phosphates (PLPs) as well as six covalently bound pyrroloquinoline quinones (PQQs) per hexameric enzyme molecule. This is the second example of a pyridoxo-quinoprotein, suggesting that ot...

journal_title：FEBS letters

authors： van der Meer RA,Groen BW,Duine JA

更新日期：1989-03-27 00:00:00

abstract：:Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...

journal_title：FEBS letters

authors： Nosaka K,Onozuka M,Konno H,Akaji K

更新日期：2008-12-10 00:00:00

abstract：:Two synthetic photoactive azidoarylphosphatidylcholines were used to investigate the level of interaction between D-beta-hydroxybutyrate apodehydrogenase (apoBDH), an amphipathic membrane protein, with the hydrophobic domain of phospholipids. The two synthetic lecithins, PL I (1-myristoyl-2-12-N-(4-azido-2-nitrophenyl...

journal_title：FEBS letters

authors： el Kebbaj MS,Berrez JM,Lakhlifi T,Morpain C,Latruffe N

更新日期：1985-03-11 00:00:00

abstract：:The post-translational modification of histone proteins plays an important role in controlling cell fate by directing essentially all DNA-associated nuclear processes. Misregulation and mutation of histone modifying enzymes is a hallmark of tumorigenesis. However, how these different epigenetic modifications lead to t...

journal_title：FEBS letters

authors： Johnsen SA

更新日期：2012-06-04 00:00:00

abstract：:pMNEI, a single chain sweet protein related to monellin, has been studied by means of 1H NMR at 500 MHz. A partial sequential assignment performed by means of the MCD method allowed the determination of the secondary structure of a large portion of the beta-sheet of pMNEI that contains a likely 'sweet finger': the loo...

journal_title：FEBS letters

authors： Tancredi T,Iijima H,Saviano G,Amodeo P,Temussi PA

更新日期：1992-09-21 00:00:00

abstract：:Activator protein 1 (AP1) is a complex of Fos and Jun, and it regulates the transcription of genes possessing the AP1-binding sequence. The purpose of this study was to detect living cells that express AP1 after stimulation with a tumor promoter. The Fos and Jun components of AP1 were induced rapidly and transiently i...

journal_title：FEBS letters

authors： Shimokawa N,Miura M

更新日期：1996-06-10 00:00:00

abstract：:To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRSctRNA complexes, where the 3'-ends (adenine 76; A76) are bound to the editing...

journal_title：FEBS letters

authors： Hagiwara Y,Nureki O,Tateno M

更新日期：2009-02-18 00:00:00

abstract：:All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr ...

journal_title：FEBS letters

authors： Kato H,Sakai T,Tamura K,Minoguchi S,Shirayoshi Y,Hamada Y,Tsujimoto Y,Honjo T

更新日期：1996-10-21 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:Male germ cells specifically express paralogues of components of the general transcription apparatus including ALF a paralogue of TFIIAalpha/beta. We show that endogenous ALF is proteolytically cleaved to give alpha- and beta-subunits and we map the proteolytic cleavage site by mass spectrometry. Immunoprecipitations ...

journal_title：FEBS letters

authors： Catena R,Argentini M,Martianov I,Parello C,Brancorsini S,Parvinen M,Sassone-Corsi P,Davidson I

更新日期：2005-06-20 00:00:00

abstract：:The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by digestion of the enzyme with carboxypeptidase Y and by automated Edman degradation of the intact ...

journal_title：FEBS letters

authors： Yokoyama C,Takai T,Tanabe T

更新日期：1988-04-25 00:00:00

abstract：:We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...

journal_title：FEBS letters

authors： Aerts AM,Zabrocki P,Govaert G,Mathys J,Carmona-Gutierrez D,Madeo F,Winderickx J,Cammue BP,Thevissen K

更新日期：2009-01-05 00:00:00

abstract：:In higher plants, two types of nitrate transporters, NRT1 and NRT2, have been identified. In Arabidopsis, there are 53 NRT1 genes and 7 NRT2 genes. NRT2 are high-affinity nitrate transporters, while most members of the NRT1 family are low-affinity nitrate transporters. The exception is CHL1 (AtNRT1.1), which is a dual...

journal_title：FEBS letters

authors： Tsay YF,Chiu CC,Tsai CB,Ho CH,Hsu PK

更新日期：2007-05-25 00:00:00

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:The fat mouse strain exhibits a late-onset obesity syndrome associated with a mutation in the gene encoding carboxypeptidase E (CPE). Since CPE plays a central role in the biosynthesis of a number of regulatory peptides, including gastrin, we examined the biogenesis and processing of progastrin in fat/fat mice by meas...

journal_title：FEBS letters

authors： Lacourse KA,Friis-Hansen L,Rehfeld JF,Samuelson LC

更新日期：1997-10-13 00:00:00

abstract：:The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% ...

journal_title：FEBS letters

authors： Guo W,Roth D,Gatti E,De Camilli P,Novick P

更新日期：1997-03-10 00:00:00

abstract：:Contactin and TAG-1 are glycan phosphatidyl inositol (GPI)-anchored cell adhesion molecules that play a crucial role in the organization of axonal subdomains at the node of Ranvier of myelinating fibers. Contactin and TAG-1 mediate axo-glial selective interactions in association with Caspr-family molecules at paranode...

journal_title：FEBS letters

authors： Labasque M,Faivre-Sarrailh C

更新日期：2010-05-03 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii were used to determine the role of two energy-dissipating systems, i.e., a free fatty acid (FFA)-activated, purine nucleotide-inhibited uncoupling protein (AcUCP) and a FFA-insensitive, purine nucleotide-activated ubiquinol alternative oxidase (AcAOX), in decreasing reac...

journal_title：FEBS letters

authors： Czarna M,Jarmuszkiewicz W

更新日期：2005-06-06 00:00:00

abstract：:Anandamide loses its cannabimimetic activities upon hydrolysis to arachidonic acid and ethanolamine. So far the anandamide hydrolyzing activity widely distributed in mammalian organs has been attributed exclusively to an enzyme referred to as anandamide amidohydrolase with an optimum pH around 9. We found another enzy...

journal_title：FEBS letters

authors： Ueda N,Yamanaka K,Terasawa Y,Yamamoto S

更新日期：1999-07-09 00:00:00

abstract：:A large protein complex (approx. 2000 kDa) was found in the cytosol of the hyperthermophilic archaebacterium Staphylothermus marinus. The purified protein was shown to be a homomultimer of 93 kDa subunits, the primary structure of which was determined by nucleotide sequence analysis. The protein belongs to the family ...

journal_title：FEBS letters

authors： Cicicopol C,Peters J,Kellermann J,Baumeister W

更新日期：1994-12-19 00:00:00

abstract：:Serine/threonine phosphatases such as PP1, PP2A, and PP2B are well known to regulate the transition phase of the cell cycle. However, the function of PP2Cgamma in cell cycle progression is still unclear. In the present study, we report the characterization of PP2Cgamma in mammalian cells during the cell cycle. After r...

journal_title：FEBS letters

authors： Suh EJ,Kim TY,Kim SH

更新日期：2006-11-13 00:00:00

abstract：:Cell migration over or through the extracellular matrix (ECM) is an integral feature of both physiological and pathological processes. Regulation of the changing cell-ECM interactions involved can be effected by proteolysis and requires strict spatial and temporal targeting of proteinase activity. The versatile use of...

journal_title：FEBS letters

authors： Ellis V,Murphy G

更新日期：2001-09-28 00:00:00

abstract：:The role of TlyA, TlyB and TlyC proteins in the biology of Leptospira is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments showed that TlyC is a surface-exposed ...

journal_title：FEBS letters

authors： Carvalho E,Barbosa AS,Gómez RM,Cianciarullo AM,Hauk P,Abreu PA,Fiorini LC,Oliveira ML,Romero EC,Gonçales AP,Morais ZM,Vasconcellos SA,Ho PL

更新日期：2009-04-17 00:00:00