Abstract:
:Enzymes from extremely halophilic archaea are readily denatured in the absence of a high salt concentration. However, we have observed here that a nucleoside diphosphate kinase prepared from Halobacterium salinarum was active and stable in the absence of salt, though it has the amino acid composition characteristic of halophilic enzymes. Recombinant nucleoside diphosphate kinase expressed in Escherichia coli requires salt for activation in vitro, but once it acquires the proper folding, it no longer requires the presence of salts for its activity and stability.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ishibashi M,Tokunaga H,Hiratsuka K,Yonezawa Y,Tsurumaru H,Arakawa T,Tokunaga Mdoi
10.1016/s0014-5793(01)02292-xkeywords:
subject
Has Abstractpub_date
2001-03-30 00:00:00pages
134-8issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02292-Xjournal_volume
493pub_type
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