Abstract:
:A Synechococcus PCC7942 mutant in which the psbO gene was inactivated by insertion of a chloramphenicol interposon and which did not contain any detectable manganese stabilizing protein in immunoblot experiments, was constructed. Such a Synechococcus mutant was able to grow under photoautotrophic conditions. Isolated thylakoid membranes from the mutant required addition of CaCl2 and MnCl2 for photosynthetic O2 evolution, and the detectable L-amino acid oxidase activity in the isolated thylakoid membranes from the mutant was approximately four times higher than in wild-type thylakoids. The results are discussed with respect to our model suggesting that the water-oxidizing enzyme may have evolved from a flavoprotein with L-amino acid dehydrogenase/oxidase activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bockholt R,Masepohl B,Pistorius EKdoi
10.1016/0014-5793(91)81343-7subject
Has Abstractpub_date
1991-12-02 00:00:00pages
59-63issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)81343-7journal_volume
294pub_type
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