Abstract:
:Treatment of cultured carrot cells with dibutyryl cAMP or forskolin resulted in the appreciable decrease in extracellular K+ concentration. This decrease was found to be transient and the concentration of the ion in the culture medium restored to the original level within few minutes. The cAMP-induced decrease in K+ level in the medium was almost completely inhibited when carrot cells were incubated in the presence of K+ channel blockers, CsCl and tetraethylammonium chloride. Appreciable amounts of 45Ca2+ were discharged from 45Ca2+-loaded inside-out vesicles of carrot plasma membrane by the stimulation with cAMP, however, the release of the ion was significantly inhibited in the presence of the K+ channel blockers. The release of 45Ca2+ from the vesicles was also observed when K+ current was evoked with an ionophore, valinomycin, even in the absence of cAMP. These results suggest that the gating of some of the inward K+ channels located at plasma membrane of cultured carrot cells is controlled by cytoplasmic concentration of cAMP and the inward K+ current across the plasma membrane induced by the nucleotide elicits Ca2+ influx into the cells possibly by the activation of voltage-dependent Ca2+ channels.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kurosaki Fdoi
10.1016/s0014-5793(97)00403-1subject
Has Abstractpub_date
1997-05-12 00:00:00pages
115-9issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00403-1journal_volume
408pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The immunosuppressive agent FK-506 has received much attention due to its efficacy and potency in the areas of transplant rejection and autoimmune disease. Calcineurin, a Ca(2+)-calmodulin activated phosphatase, was recently implicated in the immunosuppressive mechanism of FK-506. In our ongoing search for superior im...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81196-7
更新日期:1993-01-25 00:00:00
abstract::Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.034
更新日期:2009-06-18 00:00:00
abstract::The initial processes of platelet activation by platelet-activating factor (PAF) were observed by stopped-flow light scattering and fluorometry. The binding of PAF to rabbit platelets did not change the membrane fluidity, though it caused the removal of calcium from internal stores and induced concomitantly platelet s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80034-5
更新日期:1986-10-20 00:00:00
abstract::MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF form...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80078-4
更新日期:1988-08-29 00:00:00
abstract::CXCR2 plays a key role in protecting the integrity of the endothelium. Emerging evidence has demonstrated that the long ncRNAs (lncRNA) Human metastasis associated lung adenocarcinoma transcript 1 (MALAT1) participates in the regulation of the pathophysiological processes. However, whether there is crosstalk between C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.046
更新日期:2015-10-07 00:00:00
abstract::Creutzfeldt-Jakob disease is a slow, infectious, progressive neurological disorder which results in human dementia. Synaptic membranes from various brain regions of guinea pigs infected with Creutzfeldt-Jakob disease show increased guanyl nucleotide- or 5-hydroxytryptamine-mediated activation of adenylate cyclase. Thi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81205-4
更新日期:1986-03-17 00:00:00
abstract::P67, a new protein binding to a specific RNA probe, was purified from radish seedlings [Echeverria, M. and Lahmy, S. (1995) Nucleic Acids Res. 23, 4963-4970]. Amino acid sequence information obtained from P67 microsequencing allowed the isolation of genes encoding P67 in radish and Airabidopsis thaliana. Immunolocalis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01935-9
更新日期:2000-09-01 00:00:00
abstract::We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02685-0
更新日期:2001-08-10 00:00:00
abstract::Conformational changes in the extradiscal regions of rhodopsin induced by illumination were investigated by modifying the visual pigment by mild treatment with cyanogen bromide prior to and after light exposure. Light induced an increased yield of cleavage of the Met bond 253-254 and a new cleavage at the Met bond 155...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81138-8
更新日期:1985-02-11 00:00:00
abstract::To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PE...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01463-9
更新日期:1997-01-20 00:00:00
abstract::The scorpion toxin peptide BeKm-1 was synthesised by fluorenylmethoxycarbonyl solid phase chemistry and folded by air oxidation. The peptide's effects on heterologous human ether-a-go-go-related gene potassium current (I(HERG)) in HEK293 cells were assessed using 'whole-cell' patch clamp. Blockade of I(HERG) by BeKm-1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00662-8
更新日期:2003-07-17 00:00:00
abstract::Multiple protein kinase C (PKC) theta species, identified in an erythroleukaemia cell line, have been characterised in terms of their molecular properties and intracellular distribution. PKCthetas localised in the detergent-soluble cell fraction have an Mr of 76 kDa (theta-76) and contain Thr538 or pThr538 in the kina...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01073-1
更新日期:2003-11-06 00:00:00
abstract::The ARG-11 gene in Saccharomyces cerevisiae encodes a protein with the characteristic features of a family of 35 related membrane proteins that are encoded in the fungal genome. Some of them are known to transport various substrates and products across the inner membranes of mitochondria, but the functions of 29 membe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00630-3
更新日期:1997-06-30 00:00:00
abstract::Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01394-0
更新日期:1999-11-05 00:00:00
abstract::Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81254-6
更新日期:1991-09-23 00:00:00
abstract::An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential gen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02615-1
更新日期:2001-07-06 00:00:00
abstract::The widely studied macrophage scavenger receptor system is known to bind both acetylated low density lipoprotein and oxidized low density lipoprotein. Although only the latter ligand has been shown to occur in vivo, acetylated low density lipoprotein is often used to evaluate the contribution of scavenger receptors to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00437-8
更新日期:1999-04-23 00:00:00
abstract::(-)-Epigallocatechin-3-O-gallate (EGCG), a polyphenol in green tea, induces apoptosis in acute myeloid leukemia (AML) cells without affecting normal cells. In this study, we observed that cGMP acts as a cell death mediator of the EGCG-induced anti-AML effect through acid sphingomyelinase activation. EGCG activated the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.041
更新日期:2013-09-17 00:00:00
abstract::Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production afte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.11.012
更新日期:2011-12-15 00:00:00
abstract::The effect of the inositol phospholipid-binding antibiotic neomycin was studied on high-affinity GTPase in human platelet membranes. At low concentrations (up to 1 mM), neomycin by itself stimulated a high-affinity GTPase. This GTPase stimulation was additive with that caused by the hormonal factors, prostaglandin E1 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80795-6
更新日期:1988-02-29 00:00:00
abstract::Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute prom...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.001
更新日期:2008-02-06 00:00:00
abstract::Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12581
更新日期:2017-03-01 00:00:00
abstract::The cardiovascular system consists of many cell types with distinct embryonic origins. Cells from an Islet1 (Isl1)-expressing progenitor population make a substantial contribution to the developing heart. We reasoned that cells derived from Isl1-expressing progenitors might contribute more widely to the cardiovascular...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.05.014
更新日期:2012-06-21 00:00:00
abstract::We have studied the intracellular distribution in vivo of glucokinase (GK) and glucokinase regulatory protein (GKRP) in livers of fasted and refed rats, using specific antibodies against both proteins and laser confocal fluorescence microscopy. GK was found predominantly in the nucleus of hepatocytes from starved rats...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01249-1
更新日期:1999-10-08 00:00:00
abstract::Mice deficient in granulocyte-macrophage colony stimulating factor (GM-CSF) develop pulmonary alveolar proteinosis (PAP). We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01668-8
更新日期:1999-01-15 00:00:00
abstract::Acid extracts of rat pituitary neuro-intermediate lobes have been shown by ion-exchange chromatography and radio-immunoassay to contain predominantly the inactive derivatives of beta-endorphin, alpha, N-acetyl beta-endorphin 1-27 and alpha, N-acetyl beta-endorphin 1-26; the biologically active form, beta-endorphin 1-3...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80778-4
更新日期:1984-10-01 00:00:00
abstract::We describe the use of a phage-displayed random pentadecamer peptide library for searching glycosphingolipid mimicking peptides. Two phage clones (AD-1 and AD-2) were selected by biopanning using monoclonal antibody AD117m, directed to lactotetraosylceramide (Lc4Cer). The amino acid sequences of the selected clones sh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01386-0
更新日期:1997-11-24 00:00:00
abstract::Rapid degradation of specific regulatory proteins plays a role in a wide range of cellular phenomena, including cell cycle progression and the regulation of cell growth and differentiation. A major mechanism of selective protein turnover in vivo involves a large multi-subunit protease known as the proteasome or multi-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01085-4
更新日期:1994-10-31 00:00:00
abstract::The mitochondrial dynamin-like GTPase Mgm1 exists as a long (l-Mgm1) and a short isoform (s-Mgm1). They both are essential for mitochondrial fusion. Here we show that the isoforms interact in a homotypic and heterotypic manner. Their submitochondrial distribution between inner boundary membrane and cristae was markedl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.053
更新日期:2009-07-07 00:00:00
abstract::Ischemia/reperfusion induces nuclear factor kappaB (NF-kappaB) and AP-1 in rat hearts after 15 min of ischemia followed by reperfusion (R) for various periods of time (15 and 30 min, 1, 2, 3, 6, 12, and 24 h). Low levels of NF-kappaB and no signal for AP-1 were detected in shams and in non-ischemic tissue distant from...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01426-3
更新日期:1997-01-13 00:00:00