Alternative splicing generates two isoforms of the alpha 2 subunit of the inhibitory glycine receptor.

abstract：:Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...

journal_title：FEBS letters

authors： Baez M,Babul J

更新日期：2009-06-18 00:00:00

abstract：:To identify the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcgamma2R), peptides derived from the membrane-distal extracellular domain (EC1) of boFcgamma2R corresponding to the homologous region of human FcalphaRI were synthesized. Binding of bovine IgG2 to the different peptides was tested by Dot-...

journal_title：FEBS letters

authors： Zhang G,Guo J,Zhou J,Wang X,Li Q,Yang Y,Shen H,Zhao D,Zhang H,Xi J,Wang L,Qiao S,Jin X

更新日期：2006-02-20 00:00:00

abstract：:The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50- to 100-times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with ...

journal_title：FEBS letters

authors： Ehrengruber MU,Geiser T,Deranleau DA

更新日期：1994-06-13 00:00:00

abstract：:Adaptation to hypoxia is essential for tumor progression. Transcriptional activation of hypoxia-regulated genes is mediated by hypoxia-inducible factor 1 (HIF-1), a heterodimer of HIF-1alpha and ARNT (Ah receptor nuclear translocator; HIF-1beta). Using representational difference analysis, we identified three novel hy...

journal_title：FEBS letters

authors： Saarikoski ST,Rivera SP,Hankinson O

更新日期：2002-10-23 00:00:00

abstract：:A novel peptidyl-prolyl cis/trans isomerase was isolated from Escherichia coli cell extract and characterized partially. Determination of the molecular mass by electrospray mass spectrometry indicated a protein of 10102 +/- 2 Da, smaller than cyclophilins or FK 506 binding proteins currently known. The specificity con...

journal_title：FEBS letters

authors： Rahfeld JU,Schierhorn A,Mann K,Fischer G

更新日期：1994-04-18 00:00:00

abstract：:Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in o...

journal_title：FEBS letters

authors： Segond N,Jullienne A,Tahri EH,Garel JM

更新日期：1984-08-20 00:00:00

abstract：:The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human hom...

journal_title：FEBS letters

authors： Altamura N,Capitanio N,Bonnefoy N,Papa S,Dujardin G

更新日期：1996-03-11 00:00:00

abstract：:A simple technique for rapid isolation of mitochondrial DNA (mtDNA) from animal cells is described. The method is based on the selective alkaline denaturation procedure of Birnboim and Doly [(1979) Nucleic Acids Res. 7, 1513-1523] and avoids the use of CsCl gradient centrifugation. The yield of mtDNA is comparable to ...

journal_title：FEBS letters

authors： Palva TK,Palva ET

更新日期：1985-11-18 00:00:00

abstract：:Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transc...

journal_title：FEBS letters

authors： Simmons KJ,Nde PN,Kleshchenko YY,Lima MF,Villalta F

更新日期：2006-04-17 00:00:00

abstract：:Complex I (NADH:ubiquinone oxidoreductase) is the first and largest protein complex of the oxidative phosphorylation. Crystal structures have elucidated the positions of most subunits of bacterial evolutionary origin in the complex, but the positions of the eukaryotic subunits are unknown. Based on the analysis of seq...

journal_title：FEBS letters

authors： Szklarczyk R,Wanschers BF,Nabuurs SB,Nouws J,Nijtmans LG,Huynen MA

更新日期：2011-03-09 00:00:00

abstract：:Methyllycaconitine (MLA) is a competitive antagonist of nicotinic acetylcholine receptors, with a remarkable preference for neuronal [125I]alpha Bgt binding sites. We have begun to investigate the structural basis of its potency and subtype selectivity. MLA is a substituted norditerpenoid alkaloid linked to a 2-(methy...

journal_title：FEBS letters

authors： Hardick DJ,Cooper G,Scott-Ward T,Blagbrough IS,Potter BV,Wonnacott S

更新日期：1995-05-22 00:00:00

abstract：:Three DNA restriction fragments designated tyrT, 102-mer and 70-mer, have been used as substrates for footprinting studies using DNase I in the presence of the structurally similar antibiotics chartreusin and elsamicin A. The sequence-selective binding sites of the antibiotics can be mapped in regions which are rich i...

journal_title：FEBS letters

authors： Salas X,Portugal J

更新日期：1991-11-04 00:00:00

abstract：:Highly purified mitochondria from potato (Solanum tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [gamma-32P]ATP only one and three p...

journal_title：FEBS letters

authors： Pical C,Fredlund KM,Petit PX,Sommarin M,Møller IM

更新日期：1993-12-27 00:00:00

abstract：:The interactions of DNA duplexes with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a potent mutacarcinogen isolated from tryptophan pyrolysate, have been studied using CD spectroscopy. The results are that (a) the spectral change of B-form DNA caused by the interaction with Trp-P-1 is biphasic, i.e. the enla...

journal_title：FEBS letters

authors： Inohara T,Tarui M,Doi M,Inoue M,Ishida T

更新日期：1993-06-21 00:00:00

abstract：:Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is...

journal_title：FEBS letters

authors： Kooistra T,van Hinsbergh V,Havekes L,Kempen HJ

更新日期：1984-05-07 00:00:00

abstract：:By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were a...

journal_title：FEBS letters

authors： Mukougawa K,Kanamoto H,Kobayashi T,Yokota A,Kohchi T

更新日期：2006-02-20 00:00:00

abstract：:We analysed the effects of high cholesterol (HC) intake and reduced apolipoprotein E (apoE) activity on tau phosphorylation and on the activities of the major tau kinases and phosphatases in brains from wild-type and apoE-knockout (apoEKO) mice. We show that HC diet potently induced intraneuronal accumulation of hyper...

journal_title：FEBS letters

authors： Rahman A,Akterin S,Flores-Morales A,Crisby M,Kivipelto M,Schultzberg M,Cedazo-Mínguez A

更新日期：2005-11-21 00:00:00

abstract：:Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced...

journal_title：FEBS letters

authors： Nabeshima Y,Mizuguchi M,Kajiyama A,Okazawa H

更新日期：2014-12-20 00:00:00

abstract：:The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....

journal_title：FEBS letters

authors： Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RW

更新日期：1995-01-02 00:00:00

abstract：:A cell culture model of blood-brain barrier (BBB, coculture of rat brain endothelial cells with rat astrocytes) was used to investigate the effect of nitric oxide (.NO) on the damage of the BBB induced by hypoxia/reoxygenation (H/R). Permeability coefficient of fluorescein across the endothelium was used as a marker o...

journal_title：FEBS letters

authors： Utepbergenov DI,Mertsch K,Sporbert A,Tenz K,Paul M,Haseloff RF,Blasig IE

更新日期：1998-03-13 00:00:00

abstract：:The muscular content of the mRNAs encoding the five subunits of the nicotonic acetylcholine receptor was measured during postnatal development in the rat. Subunit specific mRNAs show differential regulation. The levels of the alpha-, gamma- and delta-subunit specific mRNAs decrease steadily after birth, while the beta...

journal_title：FEBS letters

authors： Witzemann V,Barg B,Criado M,Stein E,Sakmann B

更新日期：1989-01-02 00:00:00

abstract：:A unique Cu,Zn-SOD was found and isolated from plaice Paralichthys olivaceus skin. Surprisingly, the properties of purified fish skin SOD were very different from those of SOD from other sources reported so far. The purified SOD was composed of four same subunits of 16 kDa and the molecular weight of the native SOD wa...

journal_title：FEBS letters

authors： Nakano T,Sato M,Takeuchi M

更新日期：1995-02-27 00:00:00

abstract：:Sequence comparisons of 5 S and other ribosomal RNAs by segments can be useful in understanding anomalous primary and secondary structures and in assessing phylogenetic relationships. In a segmented analysis, the 5'-half of the Chlamydomonas reinhardii chloroplast 5 S ribosomal RNA is found to have a very close sequen...

journal_title：FEBS letters

authors： Delihas N

更新日期：1987-09-14 00:00:00

abstract：:Gap junctions (GJs) traverse apposing membranes of neighboring cells to mediate intercellular communication by passive diffusion of signaling molecules. We have shown previously that cells endocytose GJs utilizing the clathrin machinery. Endocytosis generates cytoplasmic double-membrane vesicles termed annular gap jun...

journal_title：FEBS letters

authors： Fong JT,Nimlamool W,Falk MM

更新日期：2014-03-03 00:00:00

abstract：:The peroxisome assembly factor-1 (PAF-1) is reported here to contain the signature subsequence for a ring finger motif in its carboxyl-terminus. This conserved subsequence in PAF-1 may be the key to a gene expression regulatory pathway important in peroxisome biogenesis. ...

journal_title：FEBS letters

authors： Patarca R,Fletcher MA

更新日期：1992-11-02 00:00:00

abstract：:Analysis of nascent gamma B-crystallin peptides accumulating during in vitro translation in a rabbit reticulocyte lysate cell-free system was carried out. As a consequence of the irregular distribution of rare codons along the polypeptide chain of gamma B-crystallin, translation of the two-domain protein is a non-unif...

journal_title：FEBS letters

authors： Komar AA,Jaenicke R

更新日期：1995-12-04 00:00:00

abstract：:2-thiouracil (TU), an established antithyroid drug and melanoma-seeker, was found to selectively inhibit neuronal nitric oxide synthase (nNOS) in a competitive manner (K(i)=20 microM), being inactive on the other NOS isoforms. The drug apparently interfered with the substrate- and tetrahydrobiopterin (BH(4))-binding t...

journal_title：FEBS letters

authors： Palumbo A,d'Ischia M,Cioffi FA

更新日期：2000-11-24 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (1,4,5-InsP3) was perfused into rat dorsal root ganglion (DRG) neurons by whole-cell patch-clamp electrodes, while measuring the membrane potential. This operation evoked a transient (2-3 min) membrane hyperpolarization of about -15 mV (from -42 mV) followed by a depolarization. The membra...

journal_title：FEBS letters

authors： Wang Z,Ypey DL,Van Duijn B

更新日期：1992-06-15 00:00:00

abstract：:We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units ...

journal_title：FEBS letters

authors： Di Primo C,Rudloff I,Reigadas S,Arzumanov AA,Gait MJ,Toulmé JJ

更新日期：2007-02-20 00:00:00

abstract：:The role of lipids in the interaction of the beta-adrenergic receptor (R) with the regulatory protein (Gs) was investigated. Solubilized preparations of R and of Gs from turkey erythrocytes were delipidated by gel filtration. They were subsequently combined and reconstituted by the addition of various lipids. When rec...

journal_title：FEBS letters

authors： Kirilovsky J,Steiner-Mordoch S,Selinger Z,Schramm M

更新日期：1985-04-08 00:00:00