Abstract:
:By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were assembled with phytochromobilin, phycocyanobilin, and phycoerythrobilin. The blue-shifted difference spectra of truncated phytochromes were observed with a phycocyanobilin chromophore compared to a phytochromobilin chromophore. When the phycoerythrobilin biosynthetic enzymes were co-expressed, E. coli cells accumulated orange-fluorescent phytochrome. The metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mukougawa K,Kanamoto H,Kobayashi T,Yokota A,Kohchi Tdoi
10.1016/j.febslet.2006.01.051keywords:
subject
Has Abstractpub_date
2006-02-20 00:00:00pages
1333-8issue
5eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00104-9journal_volume
580pub_type
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