Abstract:
:Binding of cysteine proteinases of the papain superfamily (papain and cathepsins B, B-like and L) to basement membranes was studied by using the enzymatic activity of these proteinases against their specific fluorogenic substrates. Papain inactivated by E64 was used for Kd determination by competition experiments. The binding was characterized using the following parameters, the equilibrium constant, Kd, and the number of substrate sites, n, values of which were in the range of 10(-7) M and 10(12), respectively. Such results would be of significant interest for the understanding of the biological role of cysteine proteinases in tumour invasion and other types of tissue remodeling.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Guinec N,Dalet-Fumeron V,Pagano Mdoi
10.1016/0014-5793(92)81299-2keywords:
subject
Has Abstractpub_date
1992-08-24 00:00:00pages
305-8issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(92)81299-2journal_volume
308pub_type
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