Abstract:
:Prefibrillar cytotoxicity was suggested as a common amyloid characteristic. We showed two types of albebetin prefibrillar oligomers are formed during incubation at pH 7.3. Initial round-shaped oligomers consist of 10-15 molecules determined by atomic force microscopy, do not bind thioflavin-T and do not affect viability of granular neurons and SH-SY5Y cells. They are converted into ca. 30-40-mers possessing cross-beta-sheet and reducing viability of neuronal cells. Neither monomers nor fibrils possess cytotoxicity. We suggest that oligomeric size is important for stabilising cross-beta-sheet core critical for cytotoxicity. As albebetin was used as a carrier-protein for drug delivery, examination of amyloidogenicity is required prior polypeptide biomedical applications.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zamotin V,Gharibyan A,Gibanova NV,Lavrikova MA,Dolgikh DA,Kirpichnikov MP,Kostanyan IA,Morozova-Roche LAdoi
10.1016/j.febslet.2006.03.074subject
Has Abstractpub_date
2006-05-01 00:00:00pages
2451-7issue
10eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00404-2journal_volume
580pub_type
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