Abstract:
:The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]. We have partially purified the ADF kinase(s) and found it to be calcium-regulated and inhibited by N-(6-aminohexyl)-[(3)H]5-chloro-1-naphthalenesulphonamide. Immunoblotting reveals that calmodulin-like domain protein kinase(s) (CDPK) are enriched in the purified preparation and addition of anti-CDPK to in vitro phosphorylation assays results in the inhibition of ADF phosphorylation. These data strongly suggest that plant ADF is phosphorylated by CDPK(s), a class of protein kinases unique to plants and protozoa.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Allwood EG,Smertenko AP,Hussey PJdoi
10.1016/s0014-5793(01)02528-5keywords:
subject
Has Abstractpub_date
2001-06-15 00:00:00pages
97-100issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02528-5journal_volume
499pub_type
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