Abstract:
:We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate treatment significantly increased the urea-synthetic capacity as compared to that of butyrate treatment alone, possibly through the further induction of ornithine transcarbamylase expression. Therefore, the inhibition of NO production might be useful for obtaining more differentiated hepatocytes in the process of in vitro induction of hepatocyte-specific differentiation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yoon JH,Lee HS,Kim TH,Woo GH,Kim CYdoi
10.1016/s0014-5793(00)01599-4keywords:
subject
Has Abstractpub_date
2000-06-02 00:00:00pages
175-8issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01599-4journal_volume
474pub_type
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