Nucleoside analogues previously found to be inactive against HIV may be activated by simple chemical phosphorylation.

abstract：:A 15-ns molecular dynamics simulation of the fully hydrated liquid-crystalline dimyristoylphosphatidylcholine-cholesterol (DMPC-Chol) bilayer containing approximately 22 mol% Chol was carried out. The generated trajectory was analysed to investigate the mechanism of the Chol condensing effect on DMPC hydrocarbon chain...

journal_title：FEBS letters

authors： Róg T,Pasenkiewicz-Gierula M

更新日期：2001-07-27 00:00:00

abstract：:Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...

journal_title：FEBS letters

authors： Demeshkina N,Jenner L,Westhof E,Yusupov M,Yusupova G

更新日期：2013-06-27 00:00:00

abstract：:The cardiovascular system consists of many cell types with distinct embryonic origins. Cells from an Islet1 (Isl1)-expressing progenitor population make a substantial contribution to the developing heart. We reasoned that cells derived from Isl1-expressing progenitors might contribute more widely to the cardiovascular...

journal_title：FEBS letters

authors： Keenan ID,Rhee HJ,Chaudhry B,Henderson DJ

更新日期：2012-06-21 00:00:00

abstract：:Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins: Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels incre...

journal_title：FEBS letters

authors： Schmidt O,Weyer Y,Fink MJ,Müller M,Weys S,Bindreither M,Teis D

更新日期：2017-09-01 00:00:00

abstract：:The RNA helicase Rok1 plays a role in rRNA processing and in control of cell cycle progression in Saccharomyces cerevisiae. We identified two upstream open reading frames (uORFs) within the ROK1 5' untranslated region, which inhibited Rok1 translation. Mutating uATG to uAAG or generation of a premature stop codon in t...

journal_title：FEBS letters

authors： Jeon S,Kim J

更新日期：2010-11-19 00:00:00

abstract：:Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis. Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport. In this study we use the yeast two-hybrid assay and co-immunoprecip...

journal_title：FEBS letters

authors： Peng X,Zhang Y,Zhang H,Graner S,Williams JF,Levitt ML,Lokshin A

更新日期：1999-03-05 00:00:00

abstract：:Affinity labelling of pancreatic RNase with 4-(N-2-chloroethyl-N-methylamino)benzylamide and (N----P) N-methylimidazolide of d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as sugg...

journal_title：FEBS letters

authors： Knorre DG,Buneva VN,Baram GI,Godovikova TS,Zarytova VF

更新日期：1986-01-01 00:00:00

abstract：:While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation u...

journal_title：FEBS letters

authors： Wurm S,Paar C,Sonnleitner A,Sonnleitner M,Höglinger O,Romanin C,Wechselberger C

更新日期：2004-05-21 00:00:00

abstract：:Tau proteins are abnormally phosphorylated in Alzheimer's disease. Pathological Tau proteins named PHF-Tau 55, PHF-Tau 64, and PHF-Tau 69, are the main constituents of the paired helical filaments (PHF). When treating SKNSH-SY 5Y cells with okadaic acid (OA), Tau 55 protein was clearly induced whereas Tau 64 protein w...

journal_title：FEBS letters

authors： Dupont-Wallois L,Sautière PE,Cocquerelle C,Bailleul B,Delacourte A,Caillet-Boudin ML

更新日期：1995-01-03 00:00:00

abstract：:VASP (vasodilator-stimulated phosphoprotein), a protein associated with microfilaments at cellular contact sites, has been identified as a ligand for profilin and zyxin, two proteins also involved in microfilament dynamics and organization at these regions. Here, we report that VASP also directly binds to vinculin, an...

journal_title：FEBS letters

authors： Reinhard M,Rüdiger M,Jockusch BM,Walter U

更新日期：1996-12-09 00:00:00

abstract：:Stimulation of monocytes/macrophages with lipopolysaccharide (LPS) results in activation of nuclear factor-kappaB (NF-kappaB), which plays crucial roles in regulating expression of many genes involved in the subsequent inflammatory responses. Here, we investigated roles of transforming growth factor-beta activated kin...

journal_title：FEBS letters

authors： Irie T,Muta T,Takeshige K

更新日期：2000-02-11 00:00:00

abstract：:Redox reactions of tyrosine play key roles in many biological processes, including water oxidation and DNA synthesis. We first review the redox properties of tyrosine (and other phenols) in small molecules and related polypeptides, then report work on (H20)/(Y48)-modified Pseudomonas aeruginosa azurin. The crystal str...

journal_title：FEBS letters

authors： Warren JJ,Winkler JR,Gray HB

更新日期：2012-03-09 00:00:00

abstract：:A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...

journal_title：FEBS letters

authors： Nakamura Y,Miyata M,Shimokawa N,Ohnuma Y,Katoh R,Ogawa H,Okumura K,Nakao A

更新日期：2009-06-18 00:00:00

abstract：:Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...

journal_title：FEBS letters

authors： Kairane C,Zilmer M,Mutt V,Sillard R

更新日期：1994-05-23 00:00:00

abstract：:Superoxide reacts with nitric oxide to form peroxynitrite, a potent oxidising agent which may contribute to tissue damage in pathological situations such as inflammation and ischaemia/reperfusion. One mechanism by which oxidative stress damages tissues is the induction of a specific Cyclosporin A-sensitive mitochondri...

journal_title：FEBS letters

authors： Packer MA,Murphy MP

更新日期：1994-05-30 00:00:00

abstract：:AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain w...

journal_title：FEBS letters

authors： Marui J,Kitamoto N,Kato M,Kobayashi T,Tsukagoshi N

更新日期：2002-09-25 00:00:00

abstract：:Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein...

journal_title：FEBS letters

authors： Gao XH,Zaffagnini M,Bedhomme M,Michelet L,Cassier-Chauvat C,Decottignies P,Lemaire SD

更新日期：2010-06-03 00:00:00

abstract：:(-)-Epigallocatechin-3-O-gallate (EGCG), a polyphenol in green tea, induces apoptosis in acute myeloid leukemia (AML) cells without affecting normal cells. In this study, we observed that cGMP acts as a cell death mediator of the EGCG-induced anti-AML effect through acid sphingomyelinase activation. EGCG activated the...

journal_title：FEBS letters

authors： Kumazoe M,Kim Y,Bae J,Takai M,Murata M,Suemasu Y,Sugihara K,Yamashita S,Tsukamoto S,Huang Y,Nakahara K,Yamada K,Tachibana H

更新日期：2013-09-17 00:00:00

abstract：:The structure determination of apotransketolase and the comparison of its three-dimensional structure with that of the holoenzyme has revealed that no large conformational changes are associated with cofactor binding. Two loops at the active site are flexible in the apoenzyme which enables ThDP to reach its binding si...

journal_title：FEBS letters

authors： Sundström M,Lindqvist Y,Schneider G

更新日期：1992-11-30 00:00:00

abstract：:On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The At...

journal_title：FEBS letters

authors： Oguchi K,Liu H,Tamura K,Takahashi H

更新日期：1999-09-03 00:00:00

abstract：:Calcium channels derived from sarcoplasmic reticulum of frog skeletal muscle were fused with planar lipid bilayers. Fractional open times displayed two types of calcium dependence: (i) blockable channels showed a bell-shaped calcium dependence with an activation constant of 4.5 microM, a Hill coefficient for activatio...

journal_title：FEBS letters

authors： Bull R,Marengo JJ

更新日期：1993-10-04 00:00:00

abstract：:The rapid conversion of the carotenoid violaxanthin to zeaxanthin via antheraxanthin (xanthophyll cycle) in potato leaves exposed at 23 degrees C to a strong white light of 2000 microE.m-2.s-1 was associated with a slight inhibition of photosynthetic electron transport (as estimated from chlorophyll fluorescence measu...

journal_title：FEBS letters

authors： Sarry JE,Montillet JL,Sauvaire Y,Havaux M

更新日期：1994-10-17 00:00:00

abstract：:The photochemical reaction center of Chloroflexus (Cf.) aurantiacus, a membrane bound pigment-protein complex, has been crystallized in the presence of monodisperse polyoxyethylene detergents. The crystals possessed a pronounced polymorphism. Three different crystal forms belonging to triclinic, monoclinic and orthorh...

journal_title：FEBS letters

authors： Feick R,Ertlmaier A,Ermler U

更新日期：1996-11-04 00:00:00

abstract：:Differentiation enhancing factor (DEF) is a 29 kDa protein expressed in murine erythroleukemia (MEL) cells and active in promoting a significant increase in the rate of hexamethylenebisacetamide induced differentiation of these cells. The factor was recently shown to possess an amino acid sequence identical to that re...

journal_title：FEBS letters

authors： Melloni E,Sparatore B,Patrone M,Pessino A,Passalacqua M,Pontremoli S

更新日期：1995-07-24 00:00:00

abstract：:Ataxin-3, which is encoded by a gene that has been associated with Machado-Joseph disease, contains a catalytic N-terminal Josephin domain with deubiquitinase activity. Here, we show that the Josephin domain of ataxin 3 catalyzes endo-type cleavage of Lys48-linked polyubiquitin. Furthermore, NMR data obtained followin...

journal_title：FEBS letters

authors： Satoh T,Sumiyoshi A,Yagi-Utsumi M,Sakata E,Sasakawa H,Kurimoto E,Yamaguchi Y,Li W,Joazeiro CA,Hirokawa T,Kato K

更新日期：2014-11-28 00:00:00

abstract：:The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this ...

journal_title：FEBS letters

authors： Ledent P,Duez C,Vanhove M,Lejeune A,Fonzé E,Charlier P,Rhazi-Filali F,Thamm I,Guillaume G,Samyn B,Devreese B,Van Beeumen J,Lamotte-Brasseur J,Frère JM

更新日期：1997-08-18 00:00:00

abstract：:The Mahogany/Attractin gene (Atrn) has been proposed as a downstream mediator of Agouti signaling because yellow hair color and obesity in lethal yellow (A(y)) mice are suppressed by the mahogany (Atrn(mg)) mutation. The present study examined the distribution of Atrn mRNA in the brain and spinal cord by in situ hybri...

journal_title：FEBS letters

authors： Lu Xy,Gunn TM,Shieh Kr,Barsh GS,Akil H,Watson SJ

更新日期：1999-11-26 00:00:00

abstract：:Neurofibrillary tangles formed by abnormally aggregated tau protein are a histopathological feature of tauopathies. A tau aggregation inhibitor is a potential therapeutic agent for tauopathies. In this study, we prepared a monoclonal antibody for tau, monoclonal antibody to tau protein (Tau2r3), using as epitope the 2...

journal_title：FEBS letters

authors： Tsuchida T,Susa K,Kibiki T,Tsuchiya T,Miyamoto K,In Y,Minoura K,Taniguchi T,Ishida T,Tomoo K

更新日期：2020-04-13 00:00:00

abstract：:A strain of yeast, Hansenula mrakii, secretes a toxin that kills sensitive yeasts, such as Saccharomyces cerevisiae Monoclonal antibodies raised against the toxin had both binding and neutralizing activities. The toxin in culture media was isolated by an affinity column of monoclonal antibody. The toxin is a basic pol...

journal_title：FEBS letters

authors： Yamamoto T,Imai M,Tachibana K,Mayumi M

更新日期：1986-01-20 00:00:00

abstract：:An efficient sensing of danger and a rapid activation of the immune system are crucial for the survival of plants. Conserved pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) and endogenous molecular patterns, which are present only when the tissue is infected or damaged (damage-associated molecular pattern...

journal_title：FEBS letters

authors： De Lorenzo G,Brutus A,Savatin DV,Sicilia F,Cervone F

更新日期：2011-06-06 00:00:00