Abstract:
:Differentiation enhancing factor (DEF) is a 29 kDa protein expressed in murine erythroleukemia (MEL) cells and active in promoting a significant increase in the rate of hexamethylenebisacetamide induced differentiation of these cells. The factor was recently shown to possess an amino acid sequence identical to that reported for one of the HMG1 proteins, designated as 'amphoterin' on the basis of its highly dipolar sequence. In the present study, we have expressed DEF cDNA in an E. coli strain and found that the recombinant protein has functional properties identical to those observed with native DEF. Furthermore, we demonstrate that, following MEL cell stimulation with the chemical inducer, DEF is secreted in large amounts in the extracellular medium. In fact, the N-terminal sequence and the partial amino acid sequence of tryptic peptides from the secreted protein correspond to those of DEF isolated from the soluble fraction of resting MEL cells. These results are indicative for an extracellular localization as the site of action of DEF and suggest a novel function for proteins belonging to the HMG1 family. Finally, the early decay of DEF mRNA, in chemical induced MEL cells, support the hypothesis that the involvement of the enhancing factor occurs and is completed in the early phases of cell differentiation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Melloni E,Sparatore B,Patrone M,Pessino A,Passalacqua M,Pontremoli Sdoi
10.1016/0014-5793(95)00716-msubject
Has Abstractpub_date
1995-07-24 00:00:00pages
466-70issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(95)00716-Mjournal_volume
368pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Rat liver plasma membrane removed sialic acid from mixed bovine brain gangliosides more efficiently than from sialyllactose and orosomucoid with an optimal pH of 4.5. When individual gangliosides, each labeled with [14C]sialic acid or [3H]sphingosine, were tested, not only GD1a and GM3 but also GM2 and GM1, both of wh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80985-1
更新日期:1986-10-06 00:00:00
abstract::Boar spermadhesins are a group of seminal plasma, heparin-binding proteins which appear to be involved in sperm capacitation and gamete interaction. Using a proteolytic protection assay we have identified regions of AQN-1, AQN-3, PSP-I and AWN which remain attached to a heparin-Sepharose column following in-column dig...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01513-2
更新日期:1996-02-05 00:00:00
abstract::Mesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role of MSCs in tissue regeneration is attracting particular attention. We investigate the role of MSC exosomes in skeletal muscle regeneration. MSC exosomes promote myogenesis and angiogenesis in vitro, and muscle regene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.03.031
更新日期:2015-05-08 00:00:00
abstract::The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02662-x
更新日期:2001-08-24 00:00:00
abstract::Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects. Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent. Here, we demonstrate by resonance energy transfer (RET) techniq...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01338-5
更新日期:1996-12-16 00:00:00
abstract::The kinetics of the tRNA cycle is in itself capable of keeping the translational error level almost unaffected by amino acid starvation. There is no need to assume any yet unknown mechanism or property. Kinetic analysis shows that the concentration of aminoacyl-tRNA can stay high even for large reductions in aminoacyl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80498-2
更新日期:1987-10-19 00:00:00
abstract::Human endothelial cells exposed to H2O2 showed reduced CREP DNA binding activity, enhanced HSF activation, and no induction of NFkappaB binding activity. Interestingly, H2O2 was able to induce NFkappaB subunit p65 translocation in the nucleus. In contrast, cells exposed to TNF alpha showed enhanced CREP binding activi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01244-1
更新日期:1997-10-27 00:00:00
abstract::Peroxynitrite (ONOO-) has recently been implicated in connective tissue destruction in vivo. We have studied the effect of ONOO- on the activity of tissue inhibitor of metalloproteinase-1 (TIMP-1) in vitro. The inactivation of TIMP-1 by ONOO- was dose dependent with 50 microM ONOO- reducing the inhibitory activity of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00065-8
更新日期:1996-02-26 00:00:00
abstract::Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subu...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12886
更新日期:2017-11-01 00:00:00
abstract::In the bovine ovary there is a delay of 4-6 days between the observed maximum of oxytocin mRNA and the peak in the luteal levels of oxytocin nonapeptide. This implies a maturation process involving components of the post-translational processing pathway. In situ hybridization shows the oxytocin gene to be transcribed ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81295-4
更新日期:1987-01-01 00:00:00
abstract::Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development cor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01291-1
更新日期:1994-12-19 00:00:00
abstract::This article presents an overview of the simulation studies of the behaviour of multihaem cytochromes using theoretical/computational methodologies, with an emphasis on cytochrome c(3). It starts with the first studies using rigid molecules and continuum electrostatic models, where protonation and redox events were tr...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.10.019
更新日期:2012-03-09 00:00:00
abstract::The cyoE gene of the Escherichia coli bo-type quinol oxidase operon (cyoABCDE) has been previously shown to encode heme O synthase. To demonstrate a catalytic role of a cyoE homologue (the caaE gene) in the gene cluster for caa3-type cytochrome c oxidase of thermophilic Bacillus PS3, we have carried out genetic comple...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00889-2
更新日期:1994-09-12 00:00:00
abstract::Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y-A bonds of single-stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80899-7
更新日期:1986-09-15 00:00:00
abstract::The state of assembly of the pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes was examined after the dihydrolipoyl acyltransferase (E2) component of each enzyme system had been subjected to varying degrees of limited proteolysis. Dissociation of the dihydrolipoyl dehydrogenase (E3) component accompanied...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80979-6
更新日期:1986-10-06 00:00:00
abstract::The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin-permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80107-8
更新日期:1988-07-18 00:00:00
abstract::All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2008.04.029
更新日期:2008-06-18 00:00:00
abstract::The Ca2+-binding parvalbumin has been purified for the first time from rat kidney. Its biochemical and immunological properties were indistinguishable from the muscle counterpart. By immunohistochemical methods parvalbumin was localized in part of the distal tubule and proximal collecting duct, similar to the vitamin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80569-5
更新日期:1986-05-26 00:00:00
abstract::Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymomonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol dehydrogenase (ADH) reaction ([NADH][acetaldehyde][H(+)])/([ethanol][NAD(+)]) remains above ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.034
更新日期:2006-09-18 00:00:00
abstract::Cytochrome c oxidase is a membrane-bound redox-driven proton pump that harbors two proton-transfer pathways, D and K, which are used at different stages of the reaction cycle. Here, we address the question if a D pathway with a modified energy landscape for proton transfer could take over the role of the K pathway whe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13679
更新日期:2020-03-01 00:00:00
abstract::Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. More...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.12.022
更新日期:2015-01-30 00:00:00
abstract::Determination of the protonation state of enzymes is a challenging problem in computational biophysics largely due to the vast number of possible protonic configurations. The protonation state dynamics of respiratory complex I was investigated via Monte Carlo and asynchronous dynamics simulations and a novel eigenvect...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.09.036
更新日期:2012-03-09 00:00:00
abstract::We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00246-9
更新日期:1997-04-07 00:00:00
abstract::Acid extracts of rat pituitary neuro-intermediate lobes have been shown by ion-exchange chromatography and radio-immunoassay to contain predominantly the inactive derivatives of beta-endorphin, alpha, N-acetyl beta-endorphin 1-27 and alpha, N-acetyl beta-endorphin 1-26; the biologically active form, beta-endorphin 1-3...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80778-4
更新日期:1984-10-01 00:00:00
abstract::Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposur...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.007
更新日期:2006-12-11 00:00:00
abstract::The biosynthesis of human tumor necrosis factor (hTNF) was studied. The amino-terminal extension of the hTNF precursor (26 kDa polypeptide) was not cleaved off in a cell-free system supplemented with dog pancreas microsomes. Correct maturation of pre-hTNF was nevertheless not restricted to the macrophage system: in th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80306-4
更新日期:1986-03-03 00:00:00
abstract::The cytosolic peptidyl-prolyl cis/trans isomerase cyclophilin from pig kidney can accelerate catalytically the cis/trans isomerization of prolyl peptide bonds. One- and two-dimensional 1H NMR spectroscopy was used to prove that the polypeptide hormone calcitonin is a substrate for cyclophilin. Isomerization of only on...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81338-z
更新日期:1993-06-01 00:00:00
abstract::Immunoblotting analysis of purified human urokinase plasminogen activator (u-PA), gives a positive signal when reacted with anti-phosphotyrosine monoclonal antibodies (MoAb anti-P-Tyr); competition with o-phospho-DL-tyrosine (P-Tyr) but not o-phospho-DL-threonine or serine (P-Treo, P-Ser) completely suppresses this si...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80377-f
更新日期:1991-04-09 00:00:00
abstract::Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80070-j
更新日期:1991-01-14 00:00:00
abstract::The roles of mild uncoupling caused by free fatty acids (mediated by plant uncoupling mitochondrial protein (PUMP) and ATP/ADP carrier (AAC)) and non-coupled respiration (alternative oxidase (AO)) on H(2)O(2) formation by plant mitochondria were examined. Both laurate and oleate prevent H(2)O(2) formation dependent on...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01576-3
更新日期:2000-05-26 00:00:00