Abstract:
:Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of Gi1 alpha and the simultaneous presence of Gi2 alpha and Gi3 alpha as Gi subunits in rat myometrium. The expression of Gi proteins during gestation (days 0, 12, 21) was studied with the above antibodies. No qualitative change in the nature of Gi alpha species was observed during gestation: Gi1 alpha remained undetectable, Gi2 alpha and Gi3 alpha were both present on days 12 and 21. Of significance was the increase (160%) in the amount of Gi2 alpha at midgestation (day 12) compared to days 0 and 21. A different pattern was observed with Gi3 alpha, which decreased with advancing gestation (day 0 greater than 12 greater than 21). Immunodetection of beta subunits of G proteins indicated the presence of a 35/36 kDa doublet on days 0, 12 and 21, with an increase at midgestation. The simultaneous increase in Gi2 alpha and beta subunits may provide an explanation for the previously demonstrated alteration in adenylate cyclase stimulability detected at midgestation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tanfin Z,Goureau O,Milligan G,Harbon Sdoi
10.1016/0014-5793(91)80070-jsubject
Has Abstractpub_date
1991-01-14 00:00:00pages
4-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(91)80070-Jjournal_volume
278pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::A new GTP-binding protein serving as the specific substrate of islet-activating protein (IAP), pertussis toxin, was purified from porcine brain membranes as an alpha beta gamma-heterotrimeric structure. The alpha-subunit of the purified protein (alpha 40 beta gamma) had a molecular mass of 40 kDa and differed from tha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81521-1
更新日期:1987-03-23 00:00:00
abstract::The ∆Np63 isoform of the p53-family transcription factor Trp63 is a key regulator of mammary epithelial stem cells that is involved in breast cancer development. To investigate the role of ∆Np63 at different stages of normal mammary gland development, we generated a ∆Np63-inducible conditional knockout (cKO) mouse mod...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13703
更新日期:2020-03-01 00:00:00
abstract::Stresses that impair the function of the endoplasmic reticulum (ER) lead to an accumulation of unfolded protein in the ER. Under these conditions, the expression of a variety of genes involved in preventing the accumulation of the unfolded proteins is induced. Yeast Hrd1p is an ER stress-inducible ER membrane protein ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03660-8
更新日期:2002-12-04 00:00:00
abstract::The profiling of grapevine (Vitis vinifera L.) genes under water deficit was specifically targeted to sugar transporters. Leaf water status was characterized by physiological parameters and soluble sugars content. The expression analysis provided evidence that VvHT1 hexose transporter gene was strongly down-regulated ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.09.016
更新日期:2014-11-03 00:00:00
abstract::Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However, those GFP variants which are the most efficient donor acceptor pairs for FRET measurements show a high degree of sp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03508-1
更新日期:2002-11-06 00:00:00
abstract::We identified a yeast mutant with temperature-sensitive growth defects that were rescued by VCP expression. The mutation occurred in GPI10, which encodes a mannosyl transferase for glycosylphosphatidylinositol anchor formation in the endoplasmic reticulum, and caused a Gly469Glu substitution in Gpi10. The mutant exhib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.01.017
更新日期:2015-02-27 00:00:00
abstract::To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80706-2
更新日期:1986-07-07 00:00:00
abstract::The 5.8S and 28S rRNA sequences of the oomycete Phytophthora megasperma were determined in order to study the secondary structure of these molecules and to assess the phylogenetic position of the oomycetes among the eukaryotes. Preliminary results point to an affiliation between the oomycetes, dinoflagellates and cili...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80350-1
更新日期:1994-01-31 00:00:00
abstract::The 108-residue protein E. coli thioredoxin has been uniformly enriched to 50% with deuterium at all carbon-bound hydrogen positions. Isotropic mixing (i.e. TOCSY) experiments have been conducted for both the deuterated and natural-abundance samples. Using a 54 ms mixing time correlation peaks can be seen for all four...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80452-6
更新日期:1988-06-20 00:00:00
abstract::Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80644-1
更新日期:1989-06-05 00:00:00
abstract::The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00481-1
更新日期:1994-06-13 00:00:00
abstract::Synaptotagmins (Syts) are involved in Ca(2+)-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.047
更新日期:2005-09-26 00:00:00
abstract::This study was designed to investigate changes in gene expression associated with stage-specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta. The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.079
更新日期:2005-09-12 00:00:00
abstract::p21-activated protein kinase gamma-PAK phosphorylates prolactin (PRL) in rat pituitary secretory granules on Ser-177 and on the equivalent site, Ser-179, in recombinant human PRL. This is shown by comparison of phosphopeptide maps with the human PRL mutant S179D. gamma-PAK is present in rat and bovine granules as iden...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02444-4
更新日期:2002-03-27 00:00:00
abstract::The ubiquitous transcription factor Sp1 has been implicated in the mechanism which maintains CpG islands methylation-free. Plasmids containing GC boxes (Sp1 sites) were in vitro methylated at every CpG dinucleotide. After stable introduction into F9 embryonal carcinoma cells, we analysed the methylation of the sequenc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00830-3
更新日期:1995-08-21 00:00:00
abstract::Here, we used a reductant, N-acetyl-L-cysteine (NAC), to investigate the redox-sensitive step(s) in the signalling pathway from the tumor necrosis factor (TNF) receptor to nuclear factor kappaB (NF-kappaB). We found that NAC suppressed NF-kappaB activation triggered by TNF or by overexpression of either the TNF recept...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01464-2
更新日期:2000-04-28 00:00:00
abstract::Niemann-pick type C1 (NPC1) is an endo/lysosomal membrane protein involved in intracellular cholesterol trafficking, and its luminal domain C is an essential endosomal receptor for Ebola and Marburg viruses. We have determined the crystal structure of glycosylated NPC1 luminal domain C and find all seven possible site...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12089
更新日期:2016-03-01 00:00:00
abstract::Incubation of isolated rat liver nuclei with ATP, NAD+, and micromolar Ca2+ concentrations of various metal ions resulted in extensive DNA hydrolysis. Half-maximal activity occurred with 1.0 microM Ca2+ added, and saturation of the process was observed with 10 microM Ca2+. The Ca2+ (10 microM)-activated DNA fragmentat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80168-3
更新日期:1991-02-25 00:00:00
abstract::The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80643-u
更新日期:1992-06-22 00:00:00
abstract::During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80917-6
更新日期:1992-11-09 00:00:00
abstract::In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.08.021
更新日期:2014-10-16 00:00:00
abstract::We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippa...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12247
更新日期:2016-07-01 00:00:00
abstract::Yeasts with disruptions in the genes PYC1 and PYC2 encoding the isoenzymes of pyruvate carboxylase cannot grow in a glucose-ammonium medium (Stucka et al. (1991) Mol. Gen. Genet. 229, 307-315). We have isolated a dominant mutation, BPC1-1, that allows growth in this medium of yeasts with interrupted PYC1 and PYC2 gene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01337-7
更新日期:1995-12-18 00:00:00
abstract::A novel regulation type, which may be observed as an unusual kinetic 'cooperativity', is predicted for the alpha-keto acid dehydrogenase complexes. The inter-relationship of this regulation with the well-known regulatory effect of enzyme phosphorylation is discussed. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80560-h
更新日期:1993-03-22 00:00:00
abstract::Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.10.052
更新日期:2007-11-27 00:00:00
abstract::Cytosolic extracts of trout and turkey erythrocytes were tested for their immunoreactivity with polyclonal and monoclonal antibodies to retinal arrestin (S-antigen), a cytosolic protein of photoreceptor cells involved in the desensitization of rhodopsin. After adsorption or immunoaffinity chromatography of the extract...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81663-1
更新日期:1989-12-04 00:00:00
abstract::In vertebrate photoreceptors the soluble protein arrestin (45 kDa) is involved in controlling the light dependent activity of receptor proteins such as transducin or the cGMP-phosphodiesterase. Arrestin has further been identified as the retinal-S-antigen which is assumed to cause the autoimmune disease uveitis. In a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80530-g
更新日期:1991-05-06 00:00:00
abstract::PPP2R2C encodes a gamma isoform of the subunit B55 subfamily, which is a regulatory subunit of Protein phosphatase type 2A (PP2A). Our study shows that PPP2R2C is downregulated in glioma cells and human brain cancer patient samples. Overexpression of PPP2R2C inhibited cancer cell proliferation both in vitro and in viv...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.09.029
更新日期:2013-12-11 00:00:00
abstract::We studied the effect of point mutation within the putative 11th transmembrane domain (TM11) of the Na(+)/H(+) exchanger NHE1 on the plasma membrane expression. Of the 19 mutants tested, two mutants (Tyr454 or Arg458 replaced by Cys) were retained in the endoplasmic reticulum. Interestingly, Y454C was expressed on the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02348-6
更新日期:2000-12-29 00:00:00
abstract::The role of Na+/H+ exchange in protein kinase C-mediated effects in platelets was investigated by studying the effect of removal of extracellular Na+ ([Na+]e) on the different responses induced by phorbol 12-myristate 13-acetate (PMA) and 1,2-dioctanoylglycerol (diC8). None of the responses studied, namely, protein ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80908-1
更新日期:1989-07-31 00:00:00