Na+/H+ exchange is not necessary for protein kinase C-mediated effects in platelets.

abstract：:The delta-endotoxin produced by Bacillus thuringiensis ssp. entomocidus induced the release of encapsulated [14C]sucrose from reverse-phase vesicles composed of phosphatidylcholine and cholesterol. No such release was detected when the phospholipid component of the vesicles was either phosphatidylethanolamine, phospha...

journal_title：FEBS letters

authors： Yunovitz H,Yawetz A

更新日期：1988-03-28 00:00:00

abstract：:The gamma subunit of the F1 moiety of the bovine mitochondrial H(+)-ATP synthase is shown to function as a component of the gate. Addition of purified gamma subunit to F0-liposomes inhibits transmembrane proton conduction. This inhibition can be removed by the bifunctional thiol reagent diamide. Immunoblot analysis sh...

journal_title：FEBS letters

authors： Papa S,Guerrieri F,Zanotti F,Fiermonte M,Capozza G,Jirillo E

更新日期：1990-10-15 00:00:00

abstract：:Purified barley leaf ADP-glucose pyrophosphorylase, a key enzyme of the starch synthesis in the chloroplast stroma, was analysed with respect to its possible regulation by factors defining the metabolic/effector status of the chloroplast during light and dark conditions. The enzyme required 3-phosphoglyceric acid for ...

journal_title：FEBS letters

authors： Kleczkowski LA

更新日期：1999-04-01 00:00:00

abstract：:Protein import into isolated tobacco mitochondria was investigated using mitochondria from leaves harvested at different times of the day and night. Efficient import was only detected with mitochondria isolated from leaves harvested during the dark period of the growth cycle, only low levels of import were detected fr...

journal_title：FEBS letters

authors： Dessi P,Whelan J

更新日期：1997-09-29 00:00:00

abstract：:Phototaxis in the archaeon Halobacterium salinarium is mediated by a stable complex of the photoreceptor sensory rhodopsin I and its transducer HtrI, which relays the light stimulus to the signalling pathway. Removal of the cytoplasmic signalling domain of HtrI eliminated the SRI-specific motor response to light stimu...

journal_title：FEBS letters

authors： Krah M,Marwan W,Oesterhelt D

更新日期：1994-10-24 00:00:00

abstract：:Chronic indirect stimulation of fast twitch rabbit muscle induced a rapid reduction in parvalbumin concentration. When compared to the unstimulated contralateral muscle, parvalbumins were reduced to 55% following 6 days of stimulation. Prolonged stimulation further reduced parvalbumins so that they were undetectable a...

journal_title：FEBS letters

authors： Klug G,Reichmann H,Pette D

更新日期：1983-02-21 00:00:00

abstract：:We amplified, using the polymerase chain reaction and calcitonin receptor (CTR) specific primers, RNA extracted from medullary thyroid carcinoma (MTC) and the derived TT cell line. Both secrete large amounts of calcitonin. Electrophoresis of amplification products revealed, in both cases, an ethidium bromide-stained b...

journal_title：FEBS letters

authors： Frendo JL,Pichaud F,Mourroux RD,Bouizar Z,Segond N,Moukhtar MS,Jullienne A

更新日期：1994-04-04 00:00:00

abstract：:Respiratory supercomplex factor (Rcf) 1 is a membrane-bound protein that modulates the activity of cytochrome c oxidase (CytcO) in Saccharomyces cerevisiae mitochondria. To investigate this regulatory mechanism, we studied the interactions of CytcO with potassium cyanide (KCN) upon removal of Rcf1. While the addition ...

journal_title：FEBS letters

authors： Rydström Lundin C,Brzezinski P

更新日期：2017-12-01 00:00:00

abstract：:Rat glutathione transferase 8-8 is one of the less abundant cytosolic glutathione transferases, accounting for approx. 1% of the total activity with 1-chloro-2,4-dinitrobenzene in liver. The enzyme is eluted at pH 6.3 upon chromatofocusing and has so far been identified in liver, kidney, lung and testis. Characteristi...

journal_title：FEBS letters

authors： Jensson H,Guthenberg C,Alin P,Mannervik B

更新日期：1986-07-28 00:00:00

abstract：:Statistically significant similarity was revealed between amino acid sequences of NTP-binding pattern-containing domains which are among the most conserved protein segments in dissimilar groups of ss and dsDNA viruses (papova-, parvo-, geminiviruses and P4 bacteriophage), and RNA viruses (picorna-, como- and nepovirus...

journal_title：FEBS letters

authors： Gorbalenya AE,Koonin EV,Wolf YI

更新日期：1990-03-12 00:00:00

abstract：:It has been hypothesized that hyperthermia promotes oxygen-centered free radical formation in cells; however, to date there is no direct evidence of this heat-induced increase in oxygen free radical flux. Using electron paramagnetic resonance (EPR) spin trapping, we sought direct evidence for free radical generation d...

journal_title：FEBS letters

authors： Flanagan SW,Moseley PL,Buettner GR

更新日期：1998-07-17 00:00:00

abstract：:The effects of ADP, ATP, citrate and EDTA on iron-dependent microsomal and liposomal lipid peroxidation, and on 59FeCl3 binding to the lipid membranes were measured. The aim was to test if initiation of lipid peroxidation is a site-specific mechanism requiring bound iron. In the absence of chelator, iron was bound to ...

journal_title：FEBS letters

authors： Vile GF,Winterbourn CC

更新日期：1987-05-04 00:00:00

abstract：:We have isolated a potent platelet aggregation inducer from the crude venom of Calloselasma rhodostoma (Malayan pit viper), termed rhodoaggretin, with a novel oligomeric structure consisting of a dimer of C-type lectin-like heterodimers. On the basis of its native molecular mass of 66 kDa, and a M(r) of 30 kDa for its...

journal_title：FEBS letters

authors： Wang R,Kong C,Kolatkar P,Chung MC

更新日期：2001-11-23 00:00:00

abstract：:Macrolides are a group of antibiotics structurally characterized by a macrocyclic lactone to which one or several deoxy-sugar moieties are attached. The sugar moieties are transferred to the different aglycones by glycosyltransferases (GTF). The OleI GTF of an oleandomycin producer, Streptomyces antibioticus, catalyze...

journal_title：FEBS letters

authors： Quirós LM,Carbajo RJ,Salas JA

更新日期：2000-07-07 00:00:00

abstract：:Combining mutation and chemical modification, we have introduced Asp, Gln, Cys, S-carboxymethylcysteine (Cax) and S-carbamoylmethylcysteine (Cam) into the positions of Glu190 and Glu201 of the beta subunit of F1-ATPase from the thermophilic Bacillus PS3. The steady-state ATPase activities of alpha 3 beta 3 gamma compl...

journal_title：FEBS letters

authors： Amano T,Tozawa K,Yoshida M,Murakami H

更新日期：1994-07-04 00:00:00

abstract：:AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain w...

journal_title：FEBS letters

authors： Marui J,Kitamoto N,Kato M,Kobayashi T,Tsukagoshi N

更新日期：2002-09-25 00:00:00

abstract：:A heat-stable activator for ADP-ribosylation factor (ARF)-dependent phospholipase D (PLD) was purified to near homogeneity from rat kidney cytosol by a sequential column chromatography. The purified activator has a molecular mass of 23 kDa on SDS-PAGE. Using a partially purified ARF-dependent PLD from rat kidney, the ...

journal_title：FEBS letters

authors： Akisue T,Jinnai H,Hitomi T,Miwa N,Yoshida K,Nakamura S

更新日期：1998-01-23 00:00:00

abstract：:DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including ...

journal_title：FEBS letters

authors： Jingami H,Mizuno N,Takahashi H,Shibahara S,Furutani Y,Imura H,Numa S

更新日期：1985-10-21 00:00:00

abstract：:Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However, those GFP variants which are the most efficient donor acceptor pairs for FRET measurements show a high degree of sp...

journal_title：FEBS letters

authors： Zimmermann T,Rietdorf J,Girod A,Georget V,Pepperkok R

更新日期：2002-11-06 00:00:00

abstract：:The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs...

journal_title：FEBS letters

authors： Imperi F,Visca P

更新日期：2013-11-01 00:00:00

abstract：:Circadian clocks are endogenous timing systems orchestrating the daily regulation of a huge variety of physiological, metabolic and behavioral processes. These clocks are important for health - in mammals, their disruption leads to a diverse number of pathologies. While genetic and biochemical approaches largely uncov...

journal_title：FEBS letters

authors： Wallach T,Kramer A

更新日期：2015-06-22 00:00:00

abstract：:A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a startin...

journal_title：FEBS letters

authors： Osborne S,Cecconato E,Griva S,Garetto F,Calogero R,Rosa C,Bonelli F

更新日期：1993-06-21 00:00:00

abstract：:The heat shock factor (HSF1) is the central regulator of the heat stress (hs) response and is required for stimulating the transcription of the hs genes and consequently the expression of heat shock proteins. To promote the polymerase II-dependent transcription of the hs genes, HSF has to communicate with the basal tr...

journal_title：FEBS letters

authors： Reindl A,Schöffl F

更新日期：1998-10-09 00:00:00

abstract：:The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation...

journal_title：FEBS letters

authors： Olsen J,Laustsen L,Troelsen J

更新日期：1994-04-11 00:00:00

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that ...

journal_title：FEBS letters

authors： Kim HL,Park MB,Park YS

更新日期：2011-10-03 00:00:00

abstract：:SecM in Escherichia coli has two functionally crucial regions. The arrest motif near the C-terminus interacts with the ribosomal exit tunnel to arrest its own translational elongation. The signal sequence at the N-terminus directs the SecM nascent polypeptide to the Sec-mediated export pathway to release the arrested ...

journal_title：FEBS letters

authors： Nakamori K,Chiba S,Ito K

更新日期：2014-08-25 00:00:00

abstract：:Using an in vitro co-culture approach comprising cultured murine superior cervical ganglia and MC3T3-E1 osteoblast-like cells, we found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca2+ mobilization and, after a lag period, osteoblastic Ca2+ mobilization. SV did not have any d...

journal_title：FEBS letters

authors： Obata K,Furuno T,Nakanishi M,Togari A

更新日期：2007-12-22 00:00:00

abstract：:Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pl...

journal_title：FEBS letters

authors： Inagaki NF,Inagaki FF,Kokudo N,Miyajima A

更新日期：2019-02-01 00:00:00

abstract：:A peroxidase with stability, chromatographic and immunoreactive properties similar to that of bovine lactoperoxidase has been partly purified from human colostrum. Hydrophobic interaction chromatography on Phenyl-Sepharose C1-4B gave a 10-fold purification with an apparent recovery of about 45%. The enzyme was quantit...

journal_title：FEBS letters

authors： Langbakk B,Flatmark T

更新日期：1984-09-03 00:00:00