Identification of a SecM segment required for export-coupled release from elongation arrest.

abstract：:Mature beta-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of beta-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, a...

journal_title：FEBS letters

authors： Mencarelli S,Cavalieri C,Magini A,Tancini B,Basso L,Lemansky P,Hasilik A,Li YT,Chigorno V,Orlacchio A,Emiliani C,Sonnino S

更新日期：2005-10-24 00:00:00

abstract：:A human insulinoma cDNA (PC2) that encodes a protein homologous to the Kex2/subtilisin-like proteinases has recently been described [1990, J. Biol. Chem. 265, 2997-3000]. In order to characterise the associated proteinase activity, mRNA encoding PC2 was synthesised in vitro and microinjected into Xenopus oocytes. The ...

journal_title：FEBS letters

authors： Shennan KI,Smeekens SP,Steiner DF,Docherty K

更新日期：1991-06-24 00:00:00

abstract：:The crystal structure at pH 4 of the complex of glucoamylase II(471) from Aspergillus awamori var. X100 with the pseudotetrasaccharide D-gluco-dihydroacarbose has been refined to an R-factor of 0.125 against data to 2.2 A resolution. The first two residues of the inhibitor bind at a position nearly identical to those ...

journal_title：FEBS letters

authors： Stoffer B,Aleshin AE,Firsov LM,Svensson B,Honzatko RB

更新日期：1995-01-16 00:00:00

abstract：:We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of...

journal_title：FEBS letters

authors： Böckmann A,Guittet E

更新日期：1997-11-24 00:00:00

abstract：:Mycobacterium tuberculosis contains multiple versions of the accA and accD genes that encode the alpha- and beta-subunits of at least three distinct multi-functional acyl-CoA carboxylase complexes. Because of its proposed involvement in pathogenic M. tuberculosis survival, the high-resolution crystal structure of the ...

journal_title：FEBS letters

authors： Holton SJ,King-Scott S,Nasser Eddine A,Kaufmann SH,Wilmanns M

更新日期：2006-12-22 00:00:00

abstract：:Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...

journal_title：FEBS letters

authors： Ferry DR,Glossmann H

更新日期：1982-11-08 00:00:00

abstract：:To identify the novel genes involved in lipid metabolism and lipid droplet formation that may play important roles in Hepatitis C virus (HCV) propagation, we have screened the small interfering RNA library using cell culture derived HCV (HCVcc)-infected cells. We selected and characterized the gene encoding farnesyl-d...

journal_title：FEBS letters

authors： Park EM,Nguyen LN,Lim YS,Hwang SB

更新日期：2014-05-02 00:00:00

abstract：:Using reverse transcription followed by PCR amplification (RT-PCR), we have identified multiple messenger RNAs encoding for the neuronal pore-forming Ca(2+) channel subunits alpha(1A) (P/Q channel), alpha(1B) (N channel), alpha(1D) (neuronal/endocrine L channel), alpha(1E) (R channel), alpha(1G-H) (T channel) and alph...

journal_title：FEBS letters

authors： García-Palomero E,Cuchillo-Ibáñez I,García AG,Renart J,Albillos A,Montiel C

更新日期：2000-09-22 00:00:00

abstract：:Cloned mouse delta-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid delta-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the alpha subunit of Gi1. The delta-agonist-induced current was...

journal_title：FEBS letters

authors： Miyamae T,Fukushima N,Misu Y,Ueda H

更新日期：1993-11-01 00:00:00

abstract：:Zinc (Zn) deficiency is often associated with low plasma vitamin A (retinol) concentrations. It has been suggested that the reduction in plasma retinol is secondary to reduced liver retinol binding protein (RBP) synthesis. In the present study, RBP expression was determined in HepG2 cells cultured in either Zn adequat...

journal_title：FEBS letters

authors： Satre MA,Jessen KA,Clegg MS,Keen CL

更新日期：2001-03-02 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii were used to determine the role of two energy-dissipating systems, i.e., a free fatty acid (FFA)-activated, purine nucleotide-inhibited uncoupling protein (AcUCP) and a FFA-insensitive, purine nucleotide-activated ubiquinol alternative oxidase (AcAOX), in decreasing reac...

journal_title：FEBS letters

authors： Czarna M,Jarmuszkiewicz W

更新日期：2005-06-06 00:00:00

abstract：:The purpose of the present study was to investigate whether 5-hydroxytryptamine (5-HT, serotonin) affects migration of vascular endothelial cells. 5-HT significantly enhanced migration of human aortic endothelial cells (HAECs), and this enhancement was completely inhibited by GR 55562, a 5-HT1 receptor antagonist, and...

journal_title：FEBS letters

authors： Matsusaka S,Wakabayashi I

更新日期：2005-12-19 00:00:00

abstract：:Previous electrophysiological experiments characterized the Sec61 complex, which provides the aqueous path for entry of newly-synthesized polypeptides into the mammalian endoplasmic reticulum, as a highly dynamic channel that, once activated by precursor proteins, fluctuates between main open states with mean conducta...

journal_title：FEBS letters

authors： Erdmann F,Jung M,Eyrisch S,Lang S,Helms V,Wagner R,Zimmermann R

更新日期：2009-07-21 00:00:00

abstract：:Vpu is an 81 amino acid protein encoded by HIV-1. Its role is to amplify viral release by two mechanisms: (i) docking to CD4 with the consequence of targeting CD4 for ubiquitine-mediated degradation, and (ii) formation of ion channels to enhance particle release. The intensive research on its in vivo function, combine...

journal_title：FEBS letters

authors： Fischer WB

更新日期：2003-09-18 00:00:00

abstract：:A new peptide, Tc1, containing only 23 amino acids closely packed by three disulfide bridges was isolated from the Amazonian scorpion Tityus cambridgei. It blocks reversibly the Shaker B K(+)-channels with a K(d) of 65 nM and displaces binding of noxiustoxin to mouse brain synaptosome membranes. It is the shortest kno...

journal_title：FEBS letters

authors： Batista CV,Gómez-Lagunas F,Lucas S,Possani LD

更新日期：2000-12-08 00:00:00

abstract：:Mammalian pancreatic phospholipase A2 (PLA2-I) has its specific receptor through which PLA2-I induces a variety of biological responses. In this study, a fundamental relationship between the enzymatic and the receptor-binding activities of PLA2-I was investigated. The specific binding of PLA2-I to the receptor was fou...

journal_title：FEBS letters

authors： Ishizaki J,Kishino J,Teraoka H,Ohara O,Arita H

更新日期：1993-06-21 00:00:00

abstract：:In the bovine ovary there is a delay of 4-6 days between the observed maximum of oxytocin mRNA and the peak in the luteal levels of oxytocin nonapeptide. This implies a maturation process involving components of the post-translational processing pathway. In situ hybridization shows the oxytocin gene to be transcribed ...

journal_title：FEBS letters

authors： Fehr S,Ivell R,Koll R,Schams D,Fields M,Richter D

更新日期：1987-01-01 00:00:00

abstract：:Cell stimulation raised the cytosolic free Ca2+ concentration, [Ca(2+]i and induces activation of Na+/H+ exchange which raises the cytosolic pH, pHi. Recent studies have addressed the question whether Na+/H+ exchange plays a role in Ca(2+) influx and, specifically, whether a rise in pHi alone suffices to open Ca(2+) c...

journal_title：FEBS letters

authors： Siffert W,Akkerman JW

更新日期：1989-12-18 00:00:00

abstract：:α-Synuclein (αSyn) is a key player in the pathogenesis of Parkinson's disease and other synucleinopathies. Here, we report the existence of a novel soluble α-helical conformer of αSyn, obtained through transient interaction with lipid interfaces, and propose dynamic oligomerization as the mechanism underlying its stab...

journal_title：FEBS letters

authors： Rovere M,Sanderson JB,Fonseca-Ornelas L,Patel DS,Bartels T

更新日期：2018-05-01 00:00:00

abstract：:Three homologous aspartate aminotransferases with virtually identical spatial structures and pairwise amino acid sequence identities of > 40% differ markedly with respect to the yield of renaturation upon dilution from 6 M guanidine hydrochloride (mitochondrial < cytosolic < Escherichia coli). The enzymes also respond...

journal_title：FEBS letters

authors： Widmann M,Christen P

更新日期：1995-12-27 00:00:00

abstract：:Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) plays an important role in arachidonate pathway. To investigate the contribution of cPLA(2)alpha to autoimmune diabetes, we established non-obese diabetic (NOD) mouse, an excellent model for human type 1 diabetes, deficient in cPLA(2)alpha. These mice showed severe insu...

journal_title：FEBS letters

authors： Oikawa Y,Yamato E,Tashiro F,Yamamoto M,Uozumi N,Shimada A,Shimizu T,Miyazaki J

更新日期：2005-07-18 00:00:00

abstract：:The NFkappaB family of transcription factors is regulated by inhibitory IkappaB proteins. A diversity of stimuli leads to the phosphorylation and subsequent degradation of IkappaB, releasing NFkappaB to act on its target genes. Calmodulin (CaM) is a key regulator of numerous cellular processes and is the predominant i...

journal_title：FEBS letters

authors： Hughes K,Antonsson A,Grundstrøm T

更新日期：1998-12-11 00:00:00

abstract：:Transfer of naked plasmid with insulin precursor DNA into skeletal muscle of streptozotocin (STZ)-induced diabetic mice through electroporation and detection of gene expression is described. Four different human insulin precursor DNA fragments were inserted into pcDNA3.1(-), downstream of a CMV promoter. Three of them...

journal_title：FEBS letters

authors： Yin D,Tang JG

更新日期：2001-04-20 00:00:00

abstract：:We previously obtained evidence for intrinsic aminopeptidase activity for leukotriene (LT)A4 hydrolase, an enzyme characterized to specifically catalyse the hydrolysis of LTA4 to LTB4, a chemotactic compound. From a sequence homology search between LTA4 hydrolase and several aminopeptidases, it became clear that they ...

journal_title：FEBS letters

authors： Minami M,Bito H,Ohishi N,Tsuge H,Miyano M,Mori M,Wada H,Mutoh H,Shimada S,Izumi T

更新日期：1992-09-14 00:00:00

abstract：:Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...

journal_title：FEBS letters

authors： Perálvarez A,Barnadas R,Sabés M,Querol E,Padrós E

更新日期：2001-11-23 00:00:00

abstract：:The purinergic P2X(7) receptor is an ATP-receptor channel predominantly expressed in immune cells. P2X(7) has been cloned from human, rat and mouse. Here we report cloning of the Xenopus laevis P2X(7) receptor (xP2X(7)). xP2X(7) is only about 50% identical to the mammalian homologues, shows a broad tissue expression p...

journal_title：FEBS letters

authors： Paukert M,Hidayat S,Gründer S

更新日期：2002-02-27 00:00:00

abstract：:In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...

journal_title：FEBS letters

authors： Adams NB,Brindley AA,Hunter CN,Reid JD

更新日期：2016-06-01 00:00:00

abstract：:Two free radical scavengers, bioflavonoid rutin and the copper-rutin complex Cu(Rut)Cl2, inhibited lucigenin-amplified chemiluminescence and lipid peroxidation in rat liver microsomes, Cu(Rut)Cl2 being a 5-9 times more efficient inhibitor than rutin. The enhanced inhibitory activity of Cu(Rut)Cl2 was due to the presen...

journal_title：FEBS letters

authors： Afanas'ev IB,Ostrachovich EA,Korkina LG

更新日期：1998-03-27 00:00:00

abstract：:The 4-chlorobenzoyl-CoA dehalogenase catalyzes the hydrolytic dechlorination of 4-chlorobenzoyl-CoA via a two-step mechanism, namely nucleophilic aromatic substitution and ester hydrolysis. The mutation of an active-site Histidine residue has been shown to reduce the catalytic activity in both the substitution and sub...

journal_title：FEBS letters

authors： Xu D,Guo H

更新日期：2005-08-15 00:00:00

abstract：:We describe the use of a phage-displayed random pentadecamer peptide library for searching glycosphingolipid mimicking peptides. Two phage clones (AD-1 and AD-2) were selected by biopanning using monoclonal antibody AD117m, directed to lactotetraosylceramide (Lc4Cer). The amino acid sequences of the selected clones sh...

journal_title：FEBS letters

authors： Taki T,Ishikawa D,Hamasaki H,Handa S

更新日期：1997-11-24 00:00:00