Abstract:
:The crystal structure at pH 4 of the complex of glucoamylase II(471) from Aspergillus awamori var. X100 with the pseudotetrasaccharide D-gluco-dihydroacarbose has been refined to an R-factor of 0.125 against data to 2.2 A resolution. The first two residues of the inhibitor bind at a position nearly identical to those of the closely related inhibitor acarbose in its complex with glucoamylase at pH 6. However, the electron density bifurcates beyond the second residue of the D-gluco-dihydroacarbose molecule, placing the third and fourth residues together at two positions in the active site. The position of relatively low density (estimated occupancy of 35%) corresponds to the location of the third and fourth residues of acarbose in its complex with glucoamylase at pH 6. The position of high density (65% occupancy) corresponds to a new binding mode of an extended inhibitor to the active site of glucoamylase. Presented are possible causes for the binding of D-gluco-dihydroacarbose in two conformations at the active site of glucoamylase at pH 4.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Stoffer B,Aleshin AE,Firsov LM,Svensson B,Honzatko RBdoi
10.1016/0014-5793(94)01354-4subject
Has Abstractpub_date
1995-01-16 00:00:00pages
57-61issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)01354-4journal_volume
358pub_type
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