Abstract:
:A cDNA was cloned from a rabbit spleen cDNA library which encoded a G-protein alpha subunit peptide of 374 amino acids, that at the peptide level exhibited 86% and 79% identity with human Galpha16 and mouse G(alpha)15, respectively. The rabbit G(alpha)subunit cDNA was subcloned into a mammalian expression vector and transiently co-transfected into HEK-293 cells along with cDNAs encoding the human C3a, C5a, or nociceptin/orphanin FQ receptors. In all three cases the rabbit G alpha subunit behaved similarly to G(alpha)15 or G(alpha)16 and effectively coupled the transfected receptors to intracellular calcium mobilization pathways. By nucleotide sequence homology and functional activity the rabbit G(alpha) subunit appears to be the ortholog of human G(alpha)16 and mouse G(alpha)15.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Feild JA,Foley JJ,Testa TT,Nuthulaganti P,Ellis C,Sarau HM,Ames RSdoi
10.1016/s0014-5793(99)01317-4keywords:
subject
Has Abstractpub_date
1999-10-22 00:00:00pages
53-6issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01317-4journal_volume
460pub_type
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