Regulation of the F0F1-ATP synthase: the conformation of subunit epsilon might be determined by directionality of subunit gamma rotation.

abstract：:We counted base transitions (Ts) and transversions (Tv) in mutations among various types of hepatitis C virus (HCV) sequences. The Ts/Tv ratio was useful for distinguishing the (geno)type of the compared HCV sequences, since the ratio was 4.0 or more among sequences of the same type, while it was less than 2.0 among s...

journal_title：FEBS letters

authors： Tanaka T,Kato N,Hijikata M,Shimotohno K

更新日期：1993-01-04 00:00:00

abstract：:To understand the role of nitric oxide (NO) in controlling the specific DNA-binding activities of transcriptional factors, we investigated the in vitro effect of the NO-donor sodium nitroprusside (SNP) on the AP-1 activity of cultured mouse cerebellar granule cells. A gel-mobility assay showed that SNP inhibited AP-1 ...

journal_title：FEBS letters

authors： Tabuchi A,Sano K,Oh E,Tsuchiya T,Tsuda M

更新日期：1994-08-29 00:00:00

abstract：:Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...

journal_title：FEBS letters

authors： Schmid C,Steiner T,Froesch ER

更新日期：1984-07-23 00:00:00

abstract：:Two clones were isolated by screening a shrimp hepatopancreas cDNA library with a DNA fragment obtained by PCR amplification using two oligonucleotides based on the partial protein sequence of Penaeus vanameii chymotrypsin purified earlier. One of these clones, PVC 7 contains a complete cDNA coding for a serine protea...

journal_title：FEBS letters

authors： Sellos D,Van Wormhoudt A

更新日期：1992-09-14 00:00:00

abstract：:Unlike the human blood group Sd(a+) Tamm-Horsfall glycoprotein (THGP), the Sd(a-) one lacks terminal GalNAcbeta1--> residues at the nonreducing ends. The binding properties of this glycoprotein and its asialo product with lectins were characterized by quantitative precipitin (QPA) and precipitin inhibition assays. Amo...

journal_title：FEBS letters

authors： Wu JH,Watkins WM,Chen CP,Song SC,Wu AM

更新日期：1996-04-22 00:00:00

abstract：:Fatty acid acylation of membrane proteins was studied on human erythrocytes by measuring incorporation of [3H]palmitate at different specific radioactivities. A 55 kDa polypeptide within the band 4.5 region was the main acceptor protein for acylation by fatty acids (palmitate, stearate, oleate), while other polypeptid...

journal_title：FEBS letters

authors： Maretzki D,Mariani M,Lutz HU

更新日期：1990-01-01 00:00:00

abstract：:Disruption of the gene pgil of Saccharomyces cerevisiae, which codes for phosphoglucose isomerase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6-phosphate was much higher in mutant than in wild-type cells. Phosphorylase a activity and the s...

journal_title：FEBS letters

authors： Corominas J,Clotet J,Fernández-Bañares I,Boles E,Zimmermann FK,Guinovart JJ,Ariño J

更新日期：1992-09-28 00:00:00

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:Cyanobacteria possess genes encoding phytochrome-related proteins. We used a DNA microarray approach to evaluate the impact of the phytochromes Cph1 and Cph2 on red light (R)- and far-red light (FR)-dependent gene expression in the unicellular cyanobacterium Synechocystis sp. PCC 6803. In cells of wild-type and phytoc...

journal_title：FEBS letters

authors： Hübschmann T,Yamamoto H,Gieler T,Murata N,Börner T

更新日期：2005-03-14 00:00:00

abstract：:Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...

journal_title：FEBS letters

authors： Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst J

更新日期：2000-04-14 00:00:00

abstract：:Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is k...

journal_title：FEBS letters

authors： Tamura H,Ikegami Y,Ono K,Sekimizu K,Andoh T

更新日期：1990-02-12 00:00:00

abstract：:Oncogenic mutations disrupt the regulatory circuits that govern cell function, enabling tumor cells to undergo de-regulated mitogenesis, to resist to pro-apoptotic insults, and to invade through tissue boundaries. Cancer cell biology has played a crucial role in elucidating the signaling mechanisms by which oncogenic ...

journal_title：FEBS letters

authors： Giancotti FG

更新日期：2014-08-19 00:00:00

abstract：:Bilayers made of dioleoylphosphatidylcholine (DOPC)/dipalmitoylphosphatidylcholine (DPPC) mixture containing or not cholesterol (Chl) were used to investigate the interaction of a carrier peptide with membranes. Atomic force microscopy revealed that the C-terminal 9-32 fragment of human calcitonin (hCT (9-32)), free o...

journal_title：FEBS letters

authors： Boichot S,Krauss U,Plénat T,Rennert R,Milhiet PE,Beck-Sickinger A,Le Grimellec C

更新日期：2004-07-02 00:00:00

abstract：:In human brain the Abeta peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Abeta secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which over...

journal_title：FEBS letters

authors： Recuero M,Serrano E,Bullido MJ,Valdivieso F

更新日期：2004-07-16 00:00:00

abstract：:The tailspike protein (TSP) of bacteriophage P22 is a homotrimeric multifunctional protein responsible for cell attachment and hydrolysis of the Salmonella typhimurium host cell receptor. Despite the folding of TSP involves the formation of thermolabile intermediates, the mature protein is extremely resistant to heat ...

journal_title：FEBS letters

authors： Carbonell X,Villaverde A

更新日期：1998-08-07 00:00:00

abstract：:A method was developed to study amino acids as possible precursors of PQQ biosynthesis. Cultures of Hyphomicrobium X, growing on [13C]methanol, were supplemented with unlabelled amino acids. Uptake and participation in metabolism were determined via gas chromatography/mass spectrometry of derivatized amino acids, obta...

journal_title：FEBS letters

authors： van Kleef MA,Duine JA

更新日期：1988-09-12 00:00:00

abstract：:Activation gating in KcsA is elicited by changes in intracellular proton concentration. Thompson et al. identified a charge cluster around the inner gate that plays a key role in defining proton activation in KcsA. Here, through functional and spectroscopic approaches, we confirmed the role of this charge cluster and ...

journal_title：FEBS letters

authors： Cuello LG,Cortes DM,Jogini V,Sompornpisut A,Perozo E

更新日期：2010-03-19 00:00:00

abstract：:The biological role of phosphoproteins depends upon their degree of phosphorylation in vivo. Methods currently available to measure the degree of phosphorylation of a protein involve indirect procedures to detect the 32P-phosphate incorporation. We report here a direct method to measure relative amounts of phospho- an...

journal_title：FEBS letters

authors： Di Luca M,de Graan PN,De Angelis L,Gispen WH,Cattabeni F

更新日期：1992-04-20 00:00:00

abstract：:Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...

journal_title：FEBS letters

authors： Chen JM,Stevens RA,Wray PW,Rawlings ND,Barrett AJ

更新日期：1998-09-11 00:00:00

abstract：:Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely o...

journal_title：FEBS letters

authors： Ohnuma T,Umemoto N,Kondo K,Numata T,Fukamizo T

更新日期：2013-08-19 00:00:00

abstract：:The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50- to 100-times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with ...

journal_title：FEBS letters

authors： Ehrengruber MU,Geiser T,Deranleau DA

更新日期：1994-06-13 00:00:00

abstract：:Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known o...

journal_title：FEBS letters

authors： Faoro C,Ataide SF

更新日期：2014-10-16 00:00:00

abstract：:The cyclic, imido acid containing pentapeptides cyclo(Asp-Trp-(NMe)Nle-Asp-Phe) (cpp[NMeNle(3)]) and cyclo(Asp-Trp-Pro-Asp-Phe) (cpp[Pro(3)]) have been investigated by 1H-NMR spectroscopy in DMSO and by restrained molecular dynamics methods. The spectra indicate the existence of at least four cis/trans isomers for cpp...

journal_title：FEBS letters

authors： Weisshoff H,Wieprecht T,Henklein P,Frömmel C,Antz C,Mügge C

更新日期：1996-06-03 00:00:00

abstract：:Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy o...

journal_title：FEBS letters

authors： Sugimoto N,Katoh M,Nakano S,Ohmichi T,Sasaki M

更新日期：1994-10-31 00:00:00

abstract：:Macrophages are abundant in the tumor microenvironment. They are highly plastic and able to acquire pro-tumoral phenotypes in response to microenvironmental stimuli. When we treated RAW 264.7 macrophages with inhibitors of various oncogenic pathways, we found that the focal adhesion kinase (FAK) inhibitors PF573228 an...

journal_title：FEBS letters

authors： He X,Chen X,Li B,Ji J,Chen S

更新日期：2017-12-01 00:00:00

abstract：:Bromodomains present in Brd4 and other chromatin proteins interact with acetylated histones to regulate transcription and cell growth. To study Brd4-chromatin interactions in vivo, histone H4 tail peptides were fused to a synthetic protein transduction domain (PTD) derived from the human immunodeficiency virus Tat and...

journal_title：FEBS letters

authors： Nishiyama A,Mochizuki K,Mueller F,Karpova T,McNally JG,Ozato K

更新日期：2008-04-30 00:00:00

abstract：:Two alpha-type CK2-activated PKAs (CK2-aPKAIalpha and CK2-aPKAIIalpha) were biochemically characterized in vitro using GST-HBV core fusion protein (GST-Hcore) and GST-Hcore157B as phosphate acceptors. It was found that (i), in the absence of cAMP, these two CK2-aPKAs phosphorylated both Ser-170 and Ser-178 on GST-Hcor...

journal_title：FEBS letters

authors： Enomoto M,Sawano Y,Kosuge S,Yamano Y,Kuroki K,Ohtsuki K

更新日期：2006-02-06 00:00:00

abstract：:Myeloperoxidase (MPO) is the most abundant protein in neutrophils and plays a central role in microbial killing and inflammatory tissue damage. Because most of the non-steroidal anti-inflammatory drugs and other drugs contain a thiol group, it is necessary to understand how these substrates are oxidized by MPO. We hav...

journal_title：FEBS letters

authors： Burner U,Jantschko W,Obinger C

更新日期：1999-01-29 00:00:00

abstract：:Read-through or mutations of a stop codon resulting in translation of the 3'-UTR produce potentially toxic C-terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3'-UTRs of genes associated with hereditary di...

journal_title：FEBS letters

authors： Hashimoto S,Nobuta R,Izawa T,Inada T

更新日期：2019-04-01 00:00:00

abstract：:The Na-Ca exchanger is essential for the Ca2+ homeostasis in many cell types. This transporter has been difficult to investigate because no specific inhibitor is available. We have synthesized an antisense oligodeoxynucleotide directed against the rat cardiac Na-Ca exchanger mRNA. To estimate the activity of the Na-Ca...

journal_title：FEBS letters

authors： Lipp P,Schwaller B,Niggli E

更新日期：1995-05-08 00:00:00