Abstract:
:Nerve growth factor (NGF), basic fibroblast growth factor (bFGF), dibutyryl cAMP and forskolin, known differentiating agents for pheochromocytoma PC12 cells, induced sustained activation of sphingosine kinase, the enzyme responsible for the formation of the sphingolipid second messenger, sphingosine-1-phosphate, which mediates the mitogenic effects of certain growth factors. In contrast, epidermal growth factor and insulin-like growth factor-1, which stimulate proliferation of PC12 cells, induced only small and transient increases in sphingosine kinase activity. Of the growth factors examined, NGF was the most potent activator of sphingosine kinase, inducing a 4-fold increase in Vmax. Sphingosine kinase activity induced by NGF, but not FGF, was blocked by the protein kinase inhibitor K252a when added simultaneously, with minimal effect when added after 60 min. Thus, activation of sphingosine kinase may have an important role in neural differentiation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rius RA,Edsall LC,Spiegel Sdoi
10.1016/s0014-5793(97)01277-5subject
Has Abstractpub_date
1997-11-10 00:00:00pages
173-6issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01277-5journal_volume
417pub_type
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