Evolution of putrescine N-methyltransferase from spermidine synthase demanded alterations in substrate binding.

abstract：:The intestinal microbiota is essential for nutrient acquisition, immune development, and exclusion of invading pathogens. The upper respiratory tract (URT) microbiota is less well studied and does not appear to abide by many of the paradigms of the gastrointestinal tract. Decades of carriage studies in children have d...

journal_title：FEBS letters

authors： Schenck LP,Surette MG,Bowdish DM

更新日期：2016-11-01 00:00:00

abstract：:D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

journal_title：FEBS letters

authors： Nagata Y,Homma H,Lee JA,Imai K

更新日期：1999-02-12 00:00:00

abstract：:VirG, PhoB, and OmpR are positive regulators of the virulence, phosphate, and osmolarity regulons, respectively. Their target genes are attended with one or more significant blocks of similar 6-bp sequences in their regulatory regions. The distance between the block(s) and transcriptional start site varies with the ta...

journal_title：FEBS letters

authors： Aoyama T,Oka A

更新日期：1990-04-09 00:00:00

abstract：:We have generated a mouse model for hepatic ischemia in which surgical subcutaneous transposition of the spleen allows hepatic ischemia to be applied without affecting other tissues. Using this mouse model we investigated the relationship between the length of ischemic periods in the liver and subsequent liver functio...

journal_title：FEBS letters

authors： Onishi I,Tani T,Hashimoto T,Shimizu K,Yagi M,Yamamoto K,Yoshioka K

更新日期：1997-12-29 00:00:00

abstract：:We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of...

journal_title：FEBS letters

authors： Böckmann A,Guittet E

更新日期：1997-11-24 00:00:00

abstract：:Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...

journal_title：FEBS letters

authors： Shannon MF,Wigley PL,Wells JR

更新日期：1985-07-08 00:00:00

abstract：:On April 1898 Camillo Golgi communicated to the Medical-Surgical Society of Pavia, the discovery of the "internal reticular apparatus", a novel intracellular organelle which he observed in nerve cells with the silver impregnation he had introduced for the staining of the nervous system. Soon after the discovery it bec...

journal_title：FEBS letters

authors： Mazzarello P,Garbarino C,Calligaro A

更新日期：2009-12-03 00:00:00

abstract：:The influence of poly (ADP-ribose) polymerase (PARP) and poly ADP-ribosylation on DNA synthesis supported by human replicative DNA polymerase (DNA pol) alpha, delta, and epsilon has been examined using the replication system containing poly(dA)4500-oligo(dT)12-18 as the template primer. PARP alone inhibited the pol ac...

journal_title：FEBS letters

authors： Eki T

更新日期：1994-12-19 00:00:00

abstract：:The gamma subunit of the F1 moiety of the bovine mitochondrial H(+)-ATP synthase is shown to function as a component of the gate. Addition of purified gamma subunit to F0-liposomes inhibits transmembrane proton conduction. This inhibition can be removed by the bifunctional thiol reagent diamide. Immunoblot analysis sh...

journal_title：FEBS letters

authors： Papa S,Guerrieri F,Zanotti F,Fiermonte M,Capozza G,Jirillo E

更新日期：1990-10-15 00:00:00

abstract：:We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B(12) activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear ma...

journal_title：FEBS letters

authors： Santos F,Vera JL,Lamosa P,de Valdez GF,de Vos WM,Santos H,Sesma F,Hugenholtz J

更新日期：2007-10-16 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ), a ligand-regulated nuclear hormone receptor, plays critical roles in metabolism and adipogenesis. PPARγ ligands such as thiazolidinediones (TZDs) exert insulin sensitizing and anti-inflammatory effects primarily through action on adipocytes, and are thus widely...

journal_title：FEBS letters

authors： Sugii S,Evans RM

更新日期：2011-07-07 00:00:00

abstract：:Circular dichroism (CD) spectra have been obtained from several variants of green fluorescent protein: blue fluorescent protein (BFP), enhanced cyan fluorescent protein (CFP), enhanced green fluorescent protein (GFP), enhanced yellow fluorescent protein (YFP), all from Aequorea victoria, and the red fluorescent protei...

journal_title：FEBS letters

authors： Visser NV,Hink MA,Borst JW,van der Krogt GN,Visser AJ

更新日期：2002-06-19 00:00:00

abstract：:Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the...

journal_title：FEBS letters

authors： Michel G,Rouillé Y,Chauvet MT,Chauvet J,Acher R

更新日期：1990-05-07 00:00:00

abstract：:We have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N-acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin-biotinylated peroxidase complex revealed the ...

journal_title：FEBS letters

authors： Tsuchiya E,Hiraga K,Fukui S,Miyakawa T

更新日期：1989-07-03 00:00:00

abstract：:It was believed that there was no natural suppressor tRNA in Escherichia coli, however, it has been suggested that selC, relating to the synthesis of formate dehydrogenase of a selenoprotein [(1988) Nature 331, 723-725], codes for tRNA, even though the presence of tRNA has not yet been demonstrated. We detected the pr...

journal_title：FEBS letters

authors： Mizutani T,Maruyama N,Hitaka T,Sukenaga Y

更新日期：1989-04-24 00:00:00

abstract：:The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

journal_title：FEBS letters

authors： Aspenström P

更新日期：2005-10-10 00:00:00

abstract：:cGMP has been shown to either activate or inhibit Na,K-ATPase activity. Using mouse brain endothelial cells which express both ouabain-resistant alpha1 and ouabain-sensitive alpha2 and alpha3 isoforms, we show that cGMP reduces total Na,KATPase activity to about 58%. The inhibition is prevented by the protein kinase G...

journal_title：FEBS letters

authors： Pontiggia L,Winterhalter K,Gloor SM

更新日期：1998-10-09 00:00:00

abstract：:Exposing a macrophage-like murine cell line to copper-oxidised low-density lipoprotein led to DNA fragmentation which was inhibited by the putative Ca2+/Mg2+ endonuclease inhibitor, zinc sulphate. DNA fragmentation preceded loss of membrane impermeability. These results suggest that apoptosis may be a mechanism of mac...

journal_title：FEBS letters

authors： Reid VC,Hardwick SJ,Mitchinson MJ

更新日期：1993-10-18 00:00:00

abstract：:Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...

journal_title：FEBS letters

authors： Behrensdorf HA,van de Craen M,Knies UE,Vandenabeele P,Clauss M

更新日期：2000-01-21 00:00:00

abstract：:In this work we have investigated the role of specific peptide sequences for glucose-inactivation of the yeast isocitrate lyase. Thus, different fragments of the ICL1 coding region were fused to the lacZ gene of E. coli to provide a reporter construction. Determinations of beta-galactosidase activities indicated that ...

journal_title：FEBS letters

authors： Ordiz I,Herrero P,Rodicio R,Moreno F

更新日期：1995-07-03 00:00:00

abstract：:This paper describes a mechanism of coupling periodate-oxidized nucleosides to proteins. Each of the dialdehyde groups of a periodate-oxidized nucleoside is shown to couple to lysine residues on different protein molecules through Schiff bases, thereby cross-linking different protein molecules, forming a polymer. This...

journal_title：FEBS letters

authors： Senapathy P,Ali MA,Jacob MT

更新日期：1985-10-14 00:00:00

abstract：:A mouse cDNA encoding for matrilin-3, the third member of the novel matrilin family of extracellular matrix proteins, was cloned. The protein precursor of 481 amino acids consists of a putative signal peptide, a short positively charged sequence, a single vWFA-like domain followed by four epidermal growth factor-like ...

journal_title：FEBS letters

authors： Wagener R,Kobbe B,Paulsson M

更新日期：1997-08-11 00:00:00

abstract：:Evolutionary trees based on partial small ribosomal subunit RNA sequences of 22 metazoa species have been published [(1988) Science 239, 748-753]. In these trees, cnidarians (Radiata) seemed to have evolved independently from the Bilateria, which is in contradiction with the general evolutionary view. In order to furt...

journal_title：FEBS letters

authors： Hendriks L,Van de Peer Y,Van Herck M,Neefs JM,De Wachter R

更新日期：1990-09-03 00:00:00

abstract：:The carboxy-terminal half of the c-src protein fused to the protein A moiety was expressed in bacteria. The protein A/truncated c-src fusion protein, which does not have SH2 and SH3 domains, is found in the periplasmic space allowing for a simple one-step purification and demonstrated high efficiency in autophosphoryl...

journal_title：FEBS letters

authors： Saya H,Lee PS,Nishi T,Izawa I,Nakajima M,Gallick GE,Levin VA

更新日期：1993-07-26 00:00:00

abstract：:Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a potent mitogen and migration factor for vascular smooth muscle cells (SMC), promoted neovascularization in vivo in the rabbit cornea. MRI demonstrated quantitatively the angiogenic effect of HB-EGF when introduced subcutaneously into nude mice. HB-...

journal_title：FEBS letters

authors： Abramovitch R,Neeman M,Reich R,Stein I,Keshet E,Abraham J,Solomon A,Marikovsky M

更新日期：1998-04-03 00:00:00

abstract：:It is 40 years since cancer growth was correlated with neovascularisation. Anti-angiogenic drugs remain at the forefront of cancer investigations but progress has been disappointing and unexpected toxicities are emerging. Gap junction channels are implicated in lesion spread following injury, with channel blockers sho...

journal_title：FEBS letters

authors： Zhang J,O'Carroll SJ,Henare K,Ching LM,Ormonde S,Nicholson LF,Danesh-Meyer HV,Green CR

更新日期：2014-04-17 00:00:00

abstract：:We have studied the pharmacological properties of genetically engineered human NK1 tachykinin receptors in which residues at the extracellular surface of the fourth transmembranal domain were substituted with the corresponding amino acids from the NK2 receptor. We show that substitution of G166C:Y167F in the human NK1...

journal_title：FEBS letters

authors： Ciucci A,Palma C,Riitano D,Manzini S,Werge TM

更新日期：1997-10-27 00:00:00

abstract：:G2/M checkpoints ensure the proper timing of cell mitosis. We previously reported that p38 mitogen-activated protein kinase (MAPK) activation is essential for stress-induced G2 arrest in the U-2OS osteosarcoma cell line, but the molecular mechanism was obscure. Here, using the T7 phage display system, we find p38 dire...

journal_title：FEBS letters

authors： Wu W,Zhou H,He F,Xiao Z,Jiang Y,Zhao M

更新日期：2018-12-01 00:00:00

abstract：:The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...

journal_title：FEBS letters

authors： Forsmark P,Aberg F,Norling B,Nordenbrand K,Dallner G,Ernster L

更新日期：1991-07-08 00:00:00

abstract：:The concentration of insulin that produces half-maximal stimulation of glycolysis by stripped soleus muscle preparations is markedly increased by the adenosine analogues, 2-chloroadenosine and N6-phenylisopropyladenosine, but is markedly decreased by the methyl xanthine analogue, 8-phenyltheophylline. 2-Chloroadenosin...

journal_title：FEBS letters

authors： Budohoski L,Challiss RA,McManus B,Newsholme EA

更新日期：1984-02-13 00:00:00