Abstract:
:The gamma subunit of the F1 moiety of the bovine mitochondrial H(+)-ATP synthase is shown to function as a component of the gate. Addition of purified gamma subunit to F0-liposomes inhibits transmembrane proton conduction. This inhibition can be removed by the bifunctional thiol reagent diamide. Immunoblot analysis shows that the diamide effect is likely due to disulphide bridging of the gamma subunit with the PVP protein of the F0 sector.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Papa S,Guerrieri F,Zanotti F,Fiermonte M,Capozza G,Jirillo Edoi
10.1016/0014-5793(90)80462-rsubject
Has Abstractpub_date
1990-10-15 00:00:00pages
117-20issue
1-2eissn
0014-5793issn
1873-3468journal_volume
272pub_type
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