Boric acid reversibly inhibits the second step of pre-mRNA splicing.

abstract：:Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 ...

journal_title：FEBS letters

authors： Telfer JF,Green CD

更新日期：1993-08-30 00:00:00

abstract：:Here, we investigated the molecular mechanism underlying the changes in the distribution of nucleolin. Our study identified PI3K/Akt signaling as an essential pathway regulating the distribution of nucleolin. Furthermore, nucleolin can interact with phospho-PI3K-p55, and changes in the distribution of nucleolin were r...

journal_title：FEBS letters

authors： Wu DM,Zhang P,Liu RY,Sang YX,Zhou C,Xu GC,Yang JL,Tong AP,Wang CT

更新日期：2014-05-21 00:00:00

abstract：:Apoptotic protease activating factor-1 (Apaf-1) is an adaptor molecule essential for caspase-9 activation. Subcellular analysis of Apaf-1 in NIH-3T3 fibroblasts and the immature murine B cell lymphoma WEHI-231 indicates that Apaf-1 is localized in the Golgi apparatus and cytoplasm. Bcl-2 overexpression in WEHI-231 cel...

journal_title：FEBS letters

authors： Ruiz-Vela A,Albar JP,Martínez CA

更新日期：2001-07-13 00:00:00

abstract：:We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that Ga...

journal_title：FEBS letters

authors： Alazi E,Niu J,Kowalczyk JE,Peng M,Aguilar Pontes MV,van Kan JA,Visser J,de Vries RP,Ram AF

更新日期：2016-06-01 00:00:00

abstract：:[3H]inositol-labelled products are released from adrenal medullary cells during exocytotic secretion. In 'leaky' cells in which small molecules readily enter and leave the cytoplasm, addition of micromolar calcium ions in the presence of ATP stimulates exocytosis and causes the release of inositol polyphosphates. Thes...

journal_title：FEBS letters

authors： Whitaker M

更新日期：1985-09-09 00:00:00

abstract：:Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca(2+)-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These r...

journal_title：FEBS letters

authors： Sanchez-Bueno A,Cobbold PH

更新日期：1992-11-02 00:00:00

abstract：:In the rat liver, transcription of the serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) gene occurs from two sites, +1 and +66, in exon 1, resulting in the formation of two mRNAs, one for a precursor of mitochondrial SPT/AGT and the other for peroxisomal SPT/AGT, respectively. In this study, we attempted ...

journal_title：FEBS letters

authors： Sugiyama T,Uchida C,Oda T,Kitagawa M,Hayashi H,Ichiyama A

更新日期：2001-11-09 00:00:00

abstract：:Human cystatin C (HCC) is a low molecular weight member of the cystatin family (type2). HCC consists of 120 amino acids. Normally it is an inhibitor of cysteine proteases, but in pathological conditions it forms amyloid fibrils in brain arteries of young adults. An 'aggregation-prone' pentapeptide ((47)LQVVR(51)) was ...

journal_title：FEBS letters

authors： Tsiolaki PL,Hamodrakas SJ,Iconomidou VA

更新日期：2015-01-02 00:00:00

abstract：:The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...

journal_title：FEBS letters

authors： Kuhse J,Kuryatov A,Maulet Y,Malosio ML,Schmieden V,Betz H

更新日期：1991-05-20 00:00:00

abstract：:Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase...

journal_title：FEBS letters

authors： Heldman E,Balan A,Horowitz O,Ben-Zion S,Torten M

更新日期：1985-01-28 00:00:00

abstract：:Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling pain...

journal_title：FEBS letters

authors： Biggar KK,Storey KB

更新日期：2015-11-30 00:00:00

abstract：:Immunoblotting analysis of purified human urokinase plasminogen activator (u-PA), gives a positive signal when reacted with anti-phosphotyrosine monoclonal antibodies (MoAb anti-P-Tyr); competition with o-phospho-DL-tyrosine (P-Tyr) but not o-phospho-DL-threonine or serine (P-Treo, P-Ser) completely suppresses this si...

journal_title：FEBS letters

authors： Barlati S,Paracini F,Bellotti D,De Petro G

更新日期：1991-04-09 00:00:00

abstract：:F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...

journal_title：FEBS letters

authors： Li LQ,Pan D,Chen H,Zhang L,Xie WJ

更新日期：2016-02-01 00:00:00

abstract：:The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...

journal_title：FEBS letters

authors： Oikarinen J,Mannermaa RM

更新日期：1990-10-29 00:00:00

abstract：:The extents to which different rate constants limit the steady-state rate of an isolated enzyme can be quantified as the control coefficients of those constants and elemental steps. We have found that the sum of the control coefficients of rate constants characterising unidirectional rates depleting a particular enzym...

journal_title：FEBS letters

authors： Kholodenko BN,Westerhoff HV,Brown GC

更新日期：1994-07-25 00:00:00

abstract：:Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins. In order to understand the molecular basis of these structures, we examined the binding of recombinant human tropoelastin to other extracellular matrix ligands in solid phase bindi...

journal_title：FEBS letters

authors： Sasaki T,Göhring W,Miosge N,Abrams WR,Rosenbloom J,Timpl R

更新日期：1999-10-29 00:00:00

abstract：:The galactose transport system of Saccharomyces cerevisiae consists of one component which shows a Km value of approx. 4mM in growing cells. A rapid and irreversible inactivation of this transport is detected on impairment of protein synthesis. This inactivation shows the following characteristics: (i) it is due to ch...

journal_title：FEBS letters

authors： DeJuan C,Lagunas R

更新日期：1986-10-27 00:00:00

abstract：:Ca(2+)-dependent protein kinases (CDPKs) play an essential role in plant Ca(2+)-mediated signal transduction. Twenty-nine CDPK genes have been identified in the rice genome through a complete search of genome and full-length cDNA databases. Eight of them were reported previously to be inducible by different stress sti...

journal_title：FEBS letters

authors： Wan B,Lin Y,Mou T

更新日期：2007-03-20 00:00:00

abstract：:The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic r...

journal_title：FEBS letters

authors： Tsunoda Y,Yodozawa S,Tashiro Y

更新日期：1988-04-11 00:00:00

abstract：:The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs...

journal_title：FEBS letters

authors： Imperi F,Visca P

更新日期：2013-11-01 00:00:00

abstract：:On incubation of 7-day-old wheat seedlings in the presence of [3H]thymidine, the radioactivity incorporated into coleoptile DNA is found to be localized mainly (greater than 95%) in the fraction of heavy mitochondrial DNA (H-mt DNA; rho = 1.716 gm/cm3). Upon long (48-72 h) incubation of cut-off seedlings in water, the...

journal_title：FEBS letters

authors： Kirnos MD,Alexandrushkina NI,Zagorskaya GYa,Kireev II,Vanyushin BF

更新日期：1992-02-24 00:00:00

abstract：:Histone H1(0) is found in tissues with little or no cellular proliferation and has been shown to accumulate during cellular terminal differentiation. Two subtypes of H1(0), H1 a and H1 b, are present in any tissue where the protein has been detected. We report here the first evidence of an age-dependent change in the ...

journal_title：FEBS letters

authors： Piña B,Suau P

更新日期：1987-01-05 00:00:00

abstract：:We previously identified and purified a nucleolar phosphoprotein, nucleophosmin/B23, as a stimulatory factor for replication from the adenovirus chromatin. We show here that nucleophosmin/B23 functions as a histone chaperone protein such as nucleoplasmin, TAF-I, and NAP-I. Nucleophosmin/B23 was shown to bind to histon...

journal_title：FEBS letters

authors： Okuwaki M,Matsumoto K,Tsujimoto M,Nagata K

更新日期：2001-10-12 00:00:00

abstract：:Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is known to act as a lipid phosphatase hydrolyzing phosphatidylinositol (PI)(3,4,5)P(3) to PI(4,5)P(2). Since the PI3-kinase product, PI(3,4,5)P(3), is an important second messenger leading to the metabolic action of insulin, PTEN functions as a potent neg...

journal_title：FEBS letters

authors： Ishihara H,Sasaoka T,Kagawa S,Murakami S,Fukui K,Kawagishi Y,Yamazaki K,Sato A,Iwata M,Urakaze M,Ishiki M,Wada T,Yaguchi S,Tsuneki H,Kimura I,Kobayashi M

更新日期：2003-11-20 00:00:00

abstract：:Functional analysis of heterologously expressed TaTIP2;2 by means of stopped-flow spectrometric studies provide evidence for water and ammonia conductivity. A series of experiments under increasing pH indicate that the gaseous NH(3), rather than the ammonium ion NH(4)(+) was transported. Results from inhibitor studies...

journal_title：FEBS letters

authors： Bertl A,Kaldenhoff R

更新日期：2007-11-27 00:00:00

abstract：:To search for negative regulatory components of the NF-kappaB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-kappaB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically ...

journal_title：FEBS letters

authors： Chinanonwait N,Miura H,Yamamoto N,Yamaoka S

更新日期：2002-11-20 00:00:00

abstract：:As a member of the dynamin superfamily human guanylate-binding protein 1 (hGBP1) binds and hydrolyses GTP thereby undergoing structural changes which lead to self-assembly of the protein. Here, we employ the reactivity of hGBP1 with a cysteine reactive compound in order to monitor structural changes imposed by GTP bin...

journal_title：FEBS letters

authors： Vöpel T,Kunzelmann S,Herrmann C

更新日期：2009-06-18 00:00:00

abstract：:Oncogenic mutations disrupt the regulatory circuits that govern cell function, enabling tumor cells to undergo de-regulated mitogenesis, to resist to pro-apoptotic insults, and to invade through tissue boundaries. Cancer cell biology has played a crucial role in elucidating the signaling mechanisms by which oncogenic ...

journal_title：FEBS letters

authors： Giancotti FG

更新日期：2014-08-19 00:00:00

abstract：:Initiation of translation in hepatitis C virus (HCV) is dependent on the presence of an internal ribosome entry site (IRES) contained in its 341-nt-long 5'-untranslated region (UTR). This region is very conserved among different isolates and has been used to classify HCV isolates in six different genotypes. These geno...

journal_title：FEBS letters

authors： Buratti E,Gerotto M,Pontisso P,Alberti A,Tisminetzky SG,Baralle FE

更新日期：1997-07-14 00:00:00

abstract：:The 3D structure of two unlabeled FK506 analogs, (R)- and (S)-[18-OH]ascomycin, when bound to [U-13C,15N]FKBP were determined by isotope-filtered 2D NMR experiments. The structures for the R and S isomers that bind tightly to FKBP but lack immunosuppressive activity are compared to each other and to the conformation o...

journal_title：FEBS letters

authors： Petros AM,Kawai M,Luly JR,Fesik SW

更新日期：1992-08-24 00:00:00