Abstract:
:The effects of ADP, ATP, citrate and EDTA on iron-dependent microsomal and liposomal lipid peroxidation, and on 59FeCl3 binding to the lipid membranes were measured. The aim was to test if initiation of lipid peroxidation is a site-specific mechanism requiring bound iron. In the absence of chelator, iron was bound to both membranes. EDTA and citrate removed the iron and inhibited peroxidation. ATP and ADP stimulated peroxidation, but whereas ADP allowed only half of the iron to remain bound, all was removed by ATP. Chelators, therefore, cannot be simply influencing a site-specific mechanism. Their effects must relate to the reactivities of the different iron chelates as initiators of lipid peroxidation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vile GF,Winterbourn CCdoi
10.1016/0014-5793(87)80131-xsubject
Has Abstractpub_date
1987-05-04 00:00:00pages
151-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)80131-Xjournal_volume
215pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development cor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01291-1
更新日期:1994-12-19 00:00:00
abstract::We have studied the effects of 'hydrophobic mismatch' between a poly-Leu transmembrane helix (TMH) and the ER membrane using a glycosylation mapping approach. The simplest interpretation of our results is that the lumenal end of the TMH is located deeper in the membrane for both short (negative mismatch) and long (pos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02415-2
更新日期:2001-05-11 00:00:00
abstract::The dynamic properties of 111 backbone HN sites in uncomplexed human profilin, a protein of 139 residues, have been characterized by two-dimensional inverse-detected 1H-15N NMR spectroscopy. Heteronuclear (1H)-15N nuclear Overhauser effects and 15N longitudinal and transverse relaxation rates have been analyzed in ter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80855-o
更新日期:1993-12-28 00:00:00
abstract::Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.061
更新日期:2005-10-10 00:00:00
abstract::The predominance of oxidative stress in many tumour cell environments provides a means to selectively target these cells via protein oxidation. The zinc fingers of transcription factors utilise cysteine thiols for structural zinc coordination. Redox control of DNA binding regulates transcription and therefore the over...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03890-5
更新日期:2003-01-30 00:00:00
abstract::A method was developed to study amino acids as possible precursors of PQQ biosynthesis. Cultures of Hyphomicrobium X, growing on [13C]methanol, were supplemented with unlabelled amino acids. Uptake and participation in metabolism were determined via gas chromatography/mass spectrometry of derivatized amino acids, obta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80178-9
更新日期:1988-09-12 00:00:00
abstract::The article presents the results of the first regular study of Russian populations by sequencing the control region of mitochondrial DNA (mtDNA). The sequenced region is the most variable on mtDNA molecule and is commonly used for population and evolutionary studies. Russians form one of the largest ethnic groups (mor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00115-5
更新日期:1999-02-19 00:00:00
abstract::Photoaffinity crosslinking has been utilized to probe the nature of the ligand-receptor interface for a number of G protein-coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one-at-a-time into ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.050
更新日期:2006-03-20 00:00:00
abstract::The L1CAM antibody A10-A3 efficiently reduces tumor growth in a nude mouse model. Here, we describe the crystal structure of the Fab fragment of A10-A3 determined at 2.0 angstrom resolution. The A10-A3 antibody H3 loop reveals a characteristic arrangement of exposed aromatic residues that may play an important role in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.11.028
更新日期:2011-01-03 00:00:00
abstract::Silver and gold compounds were tested as potential inhibitors of aquaporins of plant- and human origin. Silver as AgNO(3) or silver sulfadiazine inhibited with high potency (EC(50) 1-10 microM) the water permeability of the peribacteroid membrane from soybean (containing Nodulin 26), the water permeability of plasma m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03581-0
更新日期:2002-11-20 00:00:00
abstract::The triplex structure in vitro is well established; however, no direct evidence has been available concerning its existence in the cell. Using the direct chemical probing here we show that the triplex H structure can exist in E. coli cells at acidic intracellular pH values; this structure differs in some details from ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81324-h
更新日期:1990-11-12 00:00:00
abstract::An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential gen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02615-1
更新日期:2001-07-06 00:00:00
abstract::A single protein from human leukocytes possesses both 5-lipoxygenase and leukotriene A4 (LTA4) synthase activities. It has been reported that LTA4 production is more efficient when the enzyme utilizes arachidonic acid, than when 5-HPETE is exogenously supplied as substrate. In the present study, human leukocyte homoge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80675-0
更新日期:1987-06-15 00:00:00
abstract::Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived g...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02697-7
更新日期:2001-08-10 00:00:00
abstract::The antigenic cross-reactivity between purified chick, eel and mouse electrolectins (endogenous beta-D-galactoside specific lectins) have been studied using a solid phase radioimmunoassay. The immune serum raised against the eel electrolectin crossreacts both with the chick and the mouse electrolectins, while the anti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81261-1
更新日期:1982-11-01 00:00:00
abstract::Calpain, a Ca2+-dependent biomodulator, alters the properties of substrate proteins by cleaving them at a limited number of specific sites. Recent studies of the structure-function relationship of calpain and X-ray analysis of its Ca2+-binding domain have revealed hitherto unknown features of the regulation of calpain...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(98)00856-4
更新日期:1998-08-14 00:00:00
abstract::Evidence has previously suggested that cellobiose:quinone oxidoreductase (CBQ) in cellulolytic cultures of Phanerochaete chrysosporium might be produced from cellobiose oxidase (CBO) by proteolytic cleavage. This study demonstrates that the ratio of CBO activity to (CBO + CBQ) activity declines with decreasing culture...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80162-n
更新日期:1993-07-26 00:00:00
abstract::The main immunogenic region (MIR) of the acetylcholine receptor (AChR) is the target for the majority of high-affinity autoantibodies produced in myasthenia gravis patients. Some monoclonal antibodies (mAbs) to the MIR bind specifically, but with low affinity, to synthetic AChR alpha subunit peptides with the sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80286-r
更新日期:1990-07-02 00:00:00
abstract::We investigated expression levels of Na(+),K(+)-ATPase alpha-isoforms and their ATPase activities in human colorectal cancer tissue and the accompanying normal mucosa. A decrease in expression of the alpha1-isoform protein was observed in all sampled cancer tissues compared with the normal mucosae. The level of ouabai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00292-3
更新日期:2004-04-09 00:00:00
abstract::Hemocyanin is present as 2 subunits in the hemolymph of Penaeus vannamei. Isolated from a hepatopancreas cDNA library of this penaeid shrimp, the cDNA chain (2095 bp) corresponds to a full length hemocyanin messenger as determined by Northern hybridization, with a short 5' untranslated region (17 bp), an open reading ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00350-5
更新日期:1997-04-28 00:00:00
abstract::While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Here, molecular modeling and experimental measurements are used to show that neither n...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12574
更新日期:2017-03-01 00:00:00
abstract::A developmentally regulated exon has been identified in the 5'-alternatively spliced region of the human fast Troponin T (TnT) gene. Expressed in fetal (but not adult) muscle, this exon is homologous with the fetal exons recently described in the rabbit and rat fast TnT genes. They all exhibit a split codon organizati...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00729-2
更新日期:1994-08-15 00:00:00
abstract::Low-density lipoprotein receptor-related protein 4 (Lrp4) is essential for pre- and post-synaptic specialization at the neuromuscular junction (NMJ), an indispensable synapse between a motor nerve and skeletal muscle. Muscle-specific receptor tyrosine kinase MuSK must form a complex with Lrp4 to organize postsynaptic ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.10.001
更新日期:2013-11-29 00:00:00
abstract::Glucocorticoid-induced TNFR-related protein (GITR) is constitutively expressed in T regulatory (Treg) cells and regulates their suppressive function. We identified two methylated CpG islands in the Gitr locus. Using a ChIP assay, we demonstrate that both DNMT1 and methyl-CpG-binding domain Protein 4 (MBD4) bind to the...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12690
更新日期:2017-07-01 00:00:00
abstract::The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.035
更新日期:2009-06-18 00:00:00
abstract::Sarafotoxins (SRTa, SRTb and SRTc) as well as endothelin-1 (ET-1) produced vasoconstrictions in rat thoracic aorta, rat isolated perfused mesentery and pithed rat in various of extents. The potency was ET-1 greater than SRTb greater than SRTa greater than SRTc at lower doses, but SRTb greater than ET-1 greater than SR...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80120-8
更新日期:1990-01-29 00:00:00
abstract::T-kininogen is known to be an acute-phase reactant as well as a kininogen in rat plasma. Three kinds of cytokines, interleukin-1, tumor necrosis factor and interferon, were assayed for their abilities to stimulate hepatic production of T-kininogen. Of these cytokines, interferon was able to stimulate hepatic productio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81134-7
更新日期:1988-03-14 00:00:00
abstract::The relationship among the real-time trafficking of lung metastatic B16BL6 cells, metastatic potential, and the injected number of the cells was examined, since the smaller the number of tumor cells injected, the more clearly the immune defense may be observed. When 1x10(6) or 1x10(5) B16BL6 cells were injected into m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01144-3
更新日期:2000-02-11 00:00:00
abstract::Ribonuclease T1 can be unfolded and refolded without forming noticeable amounts of aggregates allowing to characterise the dimensions of a protein in different denatured states in terms of the Stokes radius RS. Upon thermal unfolding RS increases from 1.74 nm at 20 degrees C to 2.14 nm at 60 degrees C. By contrast, RS...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00058-6
更新日期:1997-02-24 00:00:00
abstract::Complexes of intact nuclear DNA with proteins undissociable by 2.0 M NaCl and nonionic detergents were analyzed by agarose gel electrophoresis following physical or enzymatic fragmentation. Sulfhydryl molecules converted these DNAs (but not the bacteriophage lambda DNA) into smaller-Mr forms. Following limited restric...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81009-7
更新日期:1985-10-21 00:00:00